全文获取类型
收费全文 | 405篇 |
免费 | 37篇 |
出版年
2022年 | 7篇 |
2021年 | 10篇 |
2020年 | 5篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 8篇 |
2016年 | 11篇 |
2015年 | 14篇 |
2014年 | 10篇 |
2013年 | 23篇 |
2012年 | 18篇 |
2011年 | 21篇 |
2010年 | 19篇 |
2009年 | 17篇 |
2008年 | 15篇 |
2007年 | 15篇 |
2006年 | 17篇 |
2005年 | 15篇 |
2004年 | 11篇 |
2003年 | 12篇 |
2002年 | 11篇 |
2001年 | 13篇 |
2000年 | 13篇 |
1999年 | 9篇 |
1998年 | 7篇 |
1995年 | 4篇 |
1994年 | 4篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 12篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 7篇 |
1982年 | 7篇 |
1981年 | 3篇 |
1980年 | 4篇 |
1979年 | 6篇 |
1978年 | 4篇 |
1973年 | 5篇 |
1972年 | 4篇 |
1971年 | 4篇 |
1969年 | 4篇 |
1967年 | 6篇 |
1966年 | 2篇 |
1965年 | 2篇 |
1919年 | 3篇 |
排序方式: 共有442条查询结果,搜索用时 31 毫秒
51.
Tang Y Finlay JA Kowalke GL Meyer AE Bright FV Callow ME Callow JA Wendt DE Detty MR 《Biofouling》2005,21(1):59-71
Hybrid sol-gel-derived xerogel films prepared from 45/55 (mol ratio) n-propyltrimethoxysilane (C3-TMOS)/tetramethylorthosilane (TMOS), 2/98 (mol ratio) bis[3-(trimethoxysilyl)propyl]-ethylenediamine (enTMOS)/tetraethylorthosilane (TEOS), 50/50 (mol ratio) n-octyltriethoxysilane (C8-TEOS)/TMOS, and 50/50 (mol ratio) 3,3,3-trifluoropropyltrimethoxysilane (TFP-TMOS)/TMOS were found to inhibit settlement of zoospores of the marine fouling alga Ulva (syn. Enteromorpha) relative to settlement on acid-washed glass and give greater release of settled zoospores relative to glass upon exposure to pressure from a water jet. The more hydrophobic 50/50 C8-TEOS/TMOS xerogel films had the lowest critical surface tension by comprehensive contact angle analysis and gave significantly greater release of 8-day Ulva sporeling biomass after exposure to turbulent flow generated by a flow channel than the other xerogel surfaces or glass. The 50/50 C8-TEOS/TMOS xerogel was also a fouling release surface for juveniles of the tropical barnacle Balanus amphitrite. X-ray photon electron data indicated that the alkylsilyl residues of the C3-TMOS-, C8-TEOS-, and TFP-TMOS-containing xerogels were located on the surface of the xerogel films (in a vacuum), which contributes to the film hydrophobicity. Similarly, the amine-containing silyl residues of the enTMOS/TEOS films were located at the surface of the xerogel films, which contributes to the more hydrophilic character and increased critical surface tension of these films. 相似文献
52.
Ohki S Baker GA Page PM McCarty TA Epand RM Bright FV 《The Journal of membrane biology》2006,211(3):191-200
The effect of lysophosphatidylcholine (LPC) on lipid vesicle fusion and leakage induced by influenza virus fusion peptides
and the peptide interaction with lipid membranes were studied by using fluorescence spectroscopy and monolayer surface tension
measurements. It was confirmed that the wild-type fusion peptide-induced vesicle fusion rate increased several-fold between
pH 7 and 5, unlike a mutated peptide, in which valine residues were substituted for glutamic acid residues at positions 11
and 15. This mutated peptide exhibited a much greater ability to induce lipid vesicle fusion and leakage but in a less pH-dependent
manner compared to the wild-type fusion peptide. The peptide-induced vesicle fusion and leakage were well correlated with
the degree of interaction of these peptides with lipid membranes, as deduced from the rotational correlation time obtained
for the peptide tryptophan fluorescence. Both vesicle fusion and leakage induced by the peptides were suppressed by LPC incorporated
into lipid vesicle membranes in a concentration-dependent manner. The rotational correlation time associated with the peptide’s
tryptophan residue, which interacts with lipid membranes containing up to 25 mole % LPC, was virtually the same compared to
lipid membranes without LPC, indicating that LPC-incorporated membrane did not affect the peptide interaction with the membrane.
The adsorption of peptide onto a lipid monolayer also showed that the presence of LPC did not affect peptide adsorption. 相似文献
53.
Little detailed information exists on the anatomy of the nervous system and the musculature of Entoprocta. Herein we describe the distribution of the neurotransmitters RFamide and serotonin as well as the myo-anatomy of adults and asexually produced budding stages of the solitary entoproct species Loxosomella vivipara and L. parguerensis using immunocytochemistry and epifluorescence as well as confocal microscopy. The development of the RFamidergic and serotonergic nervous system starts in early budding stages. In the adults, RFamide is present in the bilateral symmetric cerebral ganglion, a pair of oral nerves that innervate two pairs of nerve cell clusters in the heel of the foot, a pair of aboral nerves, the paired lateral nerves, the calyx nerves, the atrial ring nerve, the tentacle nerves, the stomach nerves, and the rectal nerves. Serotonin is only found in the cerebral ganglion, the oral nerves, and in the tentacle nerves. Some differences in the distribution of both neurotransmitters were found between L. vivipara and L. parguerensis and are most obvious in the differing number of large serotonergic perikarya associated with the oral nerves. Nerves arising from the cerebral ganglion and running in a ventral direction have not been described for Entoprocta before, and the homology of these to the ventral nerve cords of other Spiralia is considered possible. The body musculature of both Loxosomella species comprises longitudinal and diagonal muscles in the foot, the stalk, and the calyx. We found several circular muscles in the calyx. The stalk and parts of the foot and the calyx are surrounded by a fine outer layer of ring muscles. In addition to the congruent details regarding the myo-anatomy of both species, species-specific muscle structures could be revealed. The comparison of our data with recent findings of the myo-anatomy of two Loxosoma species indicates that longitudinal and diagonal body muscles, atrial ring muscles, tentacle muscles, esophageal and rectal ring muscles, as well as intestinal and anal sphincters are probably part of the ancestral entoproct muscle bauplan. 相似文献
54.
Human health risk estimates for sites with contaminated soils are often based on the assumption that the outdoor soil sieved to < 250 μm is a reasonable surrogate for predicting exposures via incidental soil ingestion. In vitro bioaccessibility tests are also increasingly used to “improve” ingestion exposure predictions of contaminants at different sites. However, when considered in terms of factors that influence desorption of contaminants from particles and uptake into humans, available studies indicate that current “standards of practice” with respect to assays of oral bioaccessibility have a number of significant shortcomings, at least in Canada. These shortcomings are discussed and various factors that influence the assessment of bioaccessibility of contaminants in soils are examined. We finish with proposing some minimum data submission requirements to support the application and relevance of bioaccessibility assays at contaminated sites. 相似文献
55.
56.
57.
The quality of seed treatment by frugivores has an effect on seed removal after dispersal, seed germination and tree recruitment. We provide information on postdispersal seed removal, germination and subsequent recruitment in tropical forest tree species Antiaris toxicaria in Ghana. We tested whether postdispersal seed removal and germination rates were differentially affected by the following seed treatments: seeds that were spat out by monkeys with all fruit pulp removed and spitting seeds with fruit pulp partially removed as observed in some birds and bats. We used seeds of intact ripened fruits as control. Frugivore seed treatment and distance from bole affected seed removal patterns, whereas intact seeds were significantly removed from all seed stations. The germination success was greater for seeds that were spat out by monkeys and poor for seeds with fruit pulp partially removed and intact fruits. More recruits were recorded at the edge of the adult A. toxicaria canopy radius. There was weak relationship (r2 = 0.042) between the number of recruits and distance away from the adult tree. Results suggest that the subsequent recruitment in tropical forest tree species may be enhanced by some frugivore fruit‐handling behaviour where fruit pulp is removed from the seeds without destroying the seeds. 相似文献
58.
The West European hedgehog, (Erinaceus europaeus, Linnaeus 1758) is widely distributed in Western Europe. However, there is evidence of decline in parts of its range. Changes
in agricultural management have partly been the driving force behind the loss of species diversity and abundance, and it has
been argued that these changes play a role in the decline of hedgehogs as well. We used a questionnaire to investigate the
current distribution of hedgehogs on farmland throughout Great Britain with a focus on different environmental zones. Additionally,
we identified environmental correlates that related to the distribution of hedgehogs with the aim to get a better understanding
of what is needed to design appropriate strategies targeted at the conservation of hedgehogs. Our study illustrates that,
although the impact of several variables was rather ambiguous, displaying positive effects in some environmental zones and
negative effects in other, major roads and Eurasian badgers (Meles meles, Linnaeus 1758) can have large scale negative effects on hedgehogs. Farm management related factors did not show a consistent
impact on hedgehog presence. Conservation strategies should therefore be aimed at lessening the impacts of major roads and
badger presence. Wildlife passages, for instance, may provide hedgehogs safe passages across roads. Additionally, increasing
the habitat complexity in order to reduce the impact of predators can be beneficial for prey species, such as hedgehogs, and
should be considered as a conservation strategy for them. 相似文献
59.
Saravanan Kanakasabai Eli Casalini Crystal C. Walline Caiqing Mo Wanida Chearwae John J. Bright 《The Journal of nutritional biochemistry》2012,23(11):1498-1507
Nutraceuticals and phytochemicals are important regulators of human health and diseases. Curcumin is a polyphenolic phytochemical isolated from the rhizome of the plant Curcuma longa (turmeric) that has been traditionally used for the treatment of inflammation and wound healing for centuries. Systematic analyses have shown that curcumin exerts its beneficial effects through antioxidant, antiproliferative and anti-inflammatory properties. We and others have shown earlier that curcumin ameliorates experimental autoimmune encephalomyelitis (EAE) model for multiple sclerosis. In this study, we show that C57BL/6 mice induced to develop EAE express elevated levels of interferon (IFN) γ and interleukin (IL)-17 in the central nervous system (CNS) and lymphoid organs that decreased significantly following in vivo treatment with curcumin. The EAE mice also showed elevated expression of IL-12 and IL-23 that decreased after treatment with curcumin. Ex vivo and in vitro treatment with curcumin resulted in a dose-dependent decrease in the secretion of IFNγ, IL-17, IL-12 and IL-23 in culture. The inhibition of EAE by curcumin was also associated with an up-regulation of IL-10, peroxisome proliferator activated receptor γ and CD4+CD25+Foxp3+ Treg cells in the CNS and lymphoid organs. These findings highlight that curcumin differentially regulates CD4+ T helper cell responses in EAE. 相似文献
60.
Bright JA 《PloS one》2012,7(2):e31769
Craniofacial sutures are a ubiquitous feature of the vertebrate skull. Previous experimental work has shown that bone strain magnitudes and orientations often vary when moving from one bone to another, across a craniofacial suture. This has led to the hypothesis that craniofacial sutures act to modify the strain environment of the skull, possibly as a mode of dissipating high stresses generated during feeding or impact. This study tests the hypothesis that the introduction of craniofacial sutures into finite element (FE) models of a modern domestic pig skull would improve model accuracy compared to a model without sutures. This allowed the mechanical effects of sutures to be assessed in isolation from other confounding variables. These models were also validated against strain gauge data collected from the same specimen ex vivo. The experimental strain data showed notable strain differences between adjacent bones, but this effect was generally not observed in either model. It was found that the inclusion of sutures in finite element models affected strain magnitudes, ratios, orientations and contour patterns, yet contrary to expectations, this did not improve the fit of the model to the experimental data, but resulted in a model that was less accurate. It is demonstrated that the presence or absence of sutures alone is not responsible for the inaccuracies in model strain, and is suggested that variations in local bone material properties, which were not accounted for by the FE models, could instead be responsible for the pattern of results. 相似文献