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31.
Ironhill, near Liphook, UK, was the site of a forest fumigation experiment. Nitrogen cycling within the humoferric podzol soil was a component of the study into the impacts of sulphur dioxide and ozone on coniferous trees. Variation in total soil N and N mineralisation was too great to determine impacts from the fumigant gases. Differences in the nitrogen mineralisation potential of the soils were unrelated to the initial levels of mineral or total N, or to pH. Mineralisation potential was affected by temperature and a Q10 of approximately 3 was demonstrated. Mineralisation potential was reduced in very dry soils, but the wetting of these dry soils did not result in enhanced mineralisation, relative to fresh samples of equivalent moisture content. Nitrification potential was detected in this forest soil of pH 3 (in 0.01 m CaCl2).The soil N data and those from the analysis of N within vegetation were used to prepare N budgets for the second and third seasons' growth of a mixed conifer forest; by the third year, N appeared to limit tree growth.The relative magnitude of proton fluxes from plant growth, nitrification and atmospheric inputs was estimated. Acidity generated from the balance of cations and anions in plant uptake, and soil N transformations was estimated to be comparable to that from `acid rain'. This comparison was based on only parts of the N cycle because they may occur remotely, in time or space, from other transformations of N. The comparison is valid, therefore, at the scale of individual trees or small-scale experimental plots, but at forest scale, wet and dry deposition were predicted to be the more significant for ecosystem acidification. 相似文献
32.
An efficient ribosomal frame-shifting signal in the polymerase-encoding region of the coronavirus IBV. 总被引:42,自引:3,他引:39
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I Brierley M E Boursnell M M Binns B Bilimoria V C Blok T D Brown S C Inglis 《The EMBO journal》1987,6(12):3779-3785
The polymerase-encoding region of the genomic RNA of the coronavirus infectious bronchitis virus (IBV) contains two very large, briefly overlapping open reading frames (ORF), F1 and F2, and it has been suggested on the basis of sequence analysis that expression of the downstream ORF, F2, might be mediated through ribosomal frame-shifting. To examine this possibility a cDNA fragment containing the F1/F2 overlap region was cloned within a marker gene and placed under the control of the bacteriophage SP6 promoter in a recombinant plasmid. Messenger RNA transcribed from this plasmid, when translated in cell-free systems, specified the synthesis of polypeptides whose size was entirely consistent with the products predicted by an efficient ribosomal frame-shifting event within the overlap region. The nature of the products was confirmed by their reactivity with antisera raised against defined portions of the flanking marker gene. This is the first non-retroviral example of ribosomal frame-shifting in higher eukaryotes. 相似文献
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Jas. Brassey Brierley 《BMJ (Clinical research ed.)》1901,2(2136):1713-1714
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I. G. Priede L. M. Williams H.-J. Wagner A. Thom I. Brierley M. A. Collins S. P. Collin N. R. Merrett C. Yau 《Proceedings. Biological sciences / The Royal Society》1999,266(1435):2295
Relative eye size, gross brain morphology and central localization of 2-[125I]iodomelatonin binding sites and melatonin receptor gene expression were compared in six gadiform fish living at different depths in the north-east Atlantic Ocean: Phycis blennoides (capture depth range 265 to 1260 m), Nezumia aequalis (445 to 1512 m), Coryphaenoides rupestris (706 to 1932 m), Trachyrincus murrayi (1010 to 1884 m), Coryphaenoides guentheri (1030 m) and Coryphaenoides (Nematonurus) armatus (2172 to 4787 m). Amongst these, the eye size range was 0.15 to 0.35 of head length with a value of 0.19 for C. (N.) armatus, the deepest species. Brain morphology reflected behavioural differences with well-developed olfactory regions in P. blennoides, T. murrayi and C. (N.) armatus and evidence of olfactory deficit in N. aequalis, C. rupestris and C. guentheri. All species had a clearly defined optic tectum with 2-[125I]iodomelatonin binding and melatonin receptor gene expression localized to specific brain regions in a similar pattern to that found in shallow-water fish. Melatonin receptors were found throughout the visual structures of the brains of all species. Despite living beyond the depth of penetration of solar light these fish have retained central features associated with the coupling of cycles of growth, behaviour and reproduction to the diel light–dark cycle. How this functions in the deep sea remains enigmatic. 相似文献
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Jas. Brassey Brierley 《BMJ (Clinical research ed.)》1908,2(2499):1590-1592
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Buecher Emmanuelle; Sparks Conrad; Brierley Andrew; Boyer Helen; Gibbons Mark 《Journal of plankton research》2001,23(10):1073-1080
Novel data on the biometry, size distribution and parasitesof Aequorea aequorea and Chrysaora hysoscella are provided frominvestigations conducted during summer and winter in the northernBenguela ecosystem. The relationship between mass and diameterof C. hysoscella did not change on a seasonal basis, and thispossibly reflects the aseasonal nature of the food environment.The changes in the size structure of C. hysoscella across theshelf and with depth agree with postulated population maintenancestrategies in the region. Aequorea aequorea was not stronglyparasitized, butC. hysoscella was subject to occasional parasitismby Hyperia medusarum, especially in winter when C. hysoscellais thought to reproduce. Parasites were distributed in a typical,negative-binomial manner on their hosts, but load was independentof host size. As medusae increased in diameter so H. medusarumtended to move from other tissues to the gonads. 相似文献
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