全文获取类型
收费全文 | 698篇 |
免费 | 76篇 |
出版年
2021年 | 9篇 |
2017年 | 10篇 |
2016年 | 10篇 |
2015年 | 12篇 |
2014年 | 12篇 |
2013年 | 27篇 |
2012年 | 34篇 |
2011年 | 26篇 |
2010年 | 19篇 |
2009年 | 13篇 |
2008年 | 26篇 |
2007年 | 13篇 |
2006年 | 15篇 |
2005年 | 14篇 |
2004年 | 10篇 |
2003年 | 9篇 |
2002年 | 18篇 |
2001年 | 27篇 |
2000年 | 12篇 |
1999年 | 18篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 10篇 |
1995年 | 14篇 |
1994年 | 9篇 |
1992年 | 13篇 |
1991年 | 11篇 |
1990年 | 16篇 |
1989年 | 12篇 |
1988年 | 18篇 |
1987年 | 19篇 |
1986年 | 16篇 |
1985年 | 15篇 |
1984年 | 14篇 |
1983年 | 10篇 |
1981年 | 20篇 |
1980年 | 8篇 |
1979年 | 22篇 |
1978年 | 13篇 |
1977年 | 13篇 |
1976年 | 17篇 |
1975年 | 10篇 |
1974年 | 7篇 |
1973年 | 14篇 |
1972年 | 13篇 |
1971年 | 9篇 |
1970年 | 11篇 |
1969年 | 9篇 |
1967年 | 7篇 |
1966年 | 7篇 |
排序方式: 共有774条查询结果,搜索用时 390 毫秒
11.
Transport of gamma-glutamyl amino acids, a step in the proposed glutathione-gamma-glutamyl transpeptidase-mediated amino acid transport pathway, was examined in mouse kidney. The transport of gamma-glutamyl amino acids was demonstrated in vitro in studies on kidney slices. Transport was followed by measuring uptake of 35S after incubation of the slices in media containing gamma-glutamyl methionine [35S]sulfone. The experimental complication associated with extracellular conversion of the gamma-glutamyl amino acid to amino acid and uptake of the latter by slices was overcome by using 5-oxoproline formation (catalyzed by intracellular gamma-glutamyl-cyclotransferase) as an indicator of gamma-glutamyl amino acid transport. This method was also successfully applied to studies on transport of gamma-glutamyl amino acids in vivo. Transport of gamma-glutamyl amino acids in vitro and in vivo is inhibited by several inhibitors of gamma-glutamyl transpeptidase and also by high extracellular levels of glutathione. This seems to explain urinary excretion of gamma-glutamylcystine by humans with gamma-glutamyl transpeptidase deficiency and by mice treated with inhibitors of this enzyme. Mice depleted of glutathione by treatment with buthionine sulfoximine (which inhibits glutathione synthesis) or by treatment with 2,6-dimethyl-2,5-heptadiene-4-one (which effectively interacts with tissue glutathione) exhibited significantly less transport of gamma-glutamyl amino acids than did untreated controls. The findings suggest that intracellular glutathione functions in transport of gamma-glutamyl amino acids. Evidence was also obtained for transport of gamma-glutamyl gamma-glutamylphenylalanine into kidney slices. 相似文献
12.
In strains of Escherichia coli deficient in excision repair (uvrA or uvrB), plasmid pKM101 muc+ but not pGW219 mucB::Tn5 enhanced resistance to angelicin monoadducts but reduced resistance to 8-methoxy-psoralen interstrand DNA crosslinks. Thermally induced recA-441 (= tif-1) bacteria showed an additional resistance to crosslinks that was blocked by pKM101. Plasmid-borne muc+ genes also conferred some additional sensitivity to gamma-radiation and it is suggested that a repair step susceptible to inhibition by muc+ gene products and possibly involving double-strand breaks may be involved after both ionizing radiation damage and psoralen crosslinks. 相似文献
13.
Characterization, Localization, and Biosynthesis of an Interstitial Retinol-Binding Glycoprotein in the Human Eye 总被引:7,自引:0,他引:7
S.-L. Fong G. I. Liou R. A. Landers R. A. Alvarez F. Gonzalez-Fernandez P. A. Glazebrook D. M. K. Lam C. D. B. Bridges 《Journal of neurochemistry》1984,42(6):1667-1676
Abstract: Human eyes contain an Mr 135K retinol-binding protein that is analogous to interstitial retinol-binding protein (IRBP) in the subretinal space of bovine eyes. It is a glycoprotein, because it binds 125 I-concanavalin A, 125 I-wheat germ agglutinin and 125 I- Lens culinaris hemagglutinin. It does not bind Ricinus communis agglutinin I. After desialation, it binds Ricinus communis agglutinin I, but loses its capacity to bind wheat germ agglutinin. These observations, coupled with the known specificities of these lectins, suggest that at least one of the oligosaccharide chains is a sialated, biantennary complex type containing fucose. Both by direct analysis of dissected ocular tissues and by immunocytochemistry it was shown that human interstitial retinol binding protein is an extracellular protein that is confined predominantly to the subretinal space. Monkey retinas incubated in vitro in medium containing [3 H]leucine were shown to synthesize and secrete this protein into the medium, a conclusion that was confirmed by immunoprecipitation with an immunoglobulin fraction prepared from rabbit antibovine IRBP serum. Virtually no other labeled proteins were detectable in the medium. It is concluded that interstitial retinol-binding protein meets many of the requirements for a putative transport protein implicated in the transfer of retinol between the pigment epithelium and retina during the visual cycle, and that the neural retina may play an important role in regulating its amount in the subretinal space. 相似文献
14.
An extracellular retinol-binding glycoprotein in the eyes of mutant rats with retinal dystrophy: development, localization, and biosynthesis 总被引:3,自引:2,他引:1 下载免费PDF全文
F Gonzalez-Fernandez R A Landers P A Glazebrook S L Fong G I Liou D M Lam C D Bridges 《The Journal of cell biology》1984,99(6):2092-2098
Interstitial retinol-binding protein (IRBP) is a soluble glycoprotein in the interphotoreceptor matrix of bovine, human, monkey, and rat eyes. It may transport retinol between the retinal pigment epithelium and the neural retina. In light-reared Royal College of Surgeons (RCS) and RCS retinal dystrophy gene (rdy)+ rats, the amount of IRBP in the interphotoreceptor matrix increased in corresponding proportion to the amount of total rhodopsin through postnatal day 22 (P22). In the RCS-rdy+ rats, the amount increased slightly after P23. However, in the RCS rats there was a rapid fall in the quantity of IRBP as the photoreceptors degenerated between P23 and P29. No IRBP was detected by immunocytochemistry in rats at P28. The amount of rhodopsin fell more slowly. Although retinas from young RCS and RCS-rdy+ rats were able to synthesize and secrete IRBP, this ability was lost in retinas from older RCS rats (P51, P88) but not their congenic controls. The photoreceptor cells have degenerated at these ages in the RCS animals, and may therefore be the retinal cells responsible for IRBP synthesis. The putative function of IRBP in the extracellular transport of retinoids during the visual cycle is consistent with a defect in retinol transport in the RCS rat reported by others. 相似文献
15.
Summary Bacteria of strain TK610 uvrA-6 his-4 umuC-36, when allowed to replicate their DNA for some hours after irradiation show induction of His+ mutations when subsequently exposed to visible light. It is suggested that base pair errors can be made opposite sites of pyrimidine dimers without involvement of umuC gene product but that the latter is required for continued replication past the dimermismatch region. Removal of the pyrimidine dimer by photoreversal allows replication to continue thus fixing the mismatched base as as mutation. 相似文献
16.
17.
Recovery of binding activity in reconstituted mouse myeloma proteins 总被引:26,自引:0,他引:26
18.
Alkaline Sucrose Gradient Sedimentation of Chromosomal Deoxyribonucleic Acid from Escherichia coli PolA+ and PolA− Strains During Thymine Starvation 下载免费PDF全文
Single-strand breaks were not detected in the deoxyribonucleic acid of Escherichia coli after thymine starvation for up to 180 min, even in a sensitive PolA(-) strain. 相似文献
19.
20.