Distribution of zooplankton associated with the Endeavour Ridge Hydrothermal Plume

Enhanced zooplankton biomass was found in shallow (<900 m)and deep (>900 m) net samples collected over the EndeavourRidge hydrothermal plume in 1991 and 1992. This enhanced biomasswas manifest in considerably higher abundance values for mostspecies in proximity to the vent field compared with abundancevalues from net samples collected 10–50 km off-axis. Thespecies most enriched in abundance by the hydrothermal plumewere those normally found in the mid-depth scattering layerat 400–900 m depth. These mid-depth animals were alsothe dominant fauna in the deep scattering layers that overliethe hydrothermal plume at depths of 1200–1900 m near themain vent field. The abundance and biomass dominants were specieswith pronounced ontogenetic migration patterns, and their associatedpredators. The abundance of many typically deep species wasalso enhanced over the main vent field. Faimal compositionsof net samples were compared using a similarity measure andaverage linkage rule. Deep fauna in proximity to the vent fieldbut not associated with scattering layers (Group 1), were similarto deep fauna 10–50 km off axis. The fauna of the deepscattering layer over the vent field (Group 2) was most similarto surface and mid- depth scattering layer fauna found withina 50km radius of the vent field. Statistical tests of linkagesobtained using a bootstrap method indicate that the abundanceand taxonomic composition of the two faunal groups were significantlydistinct in 1992, but not in 1991. We conclude that there wasconsiderable infiltration of shallow fauna into the deep scatteringlayers within 2–3 km of the main vent field, less extensiveinfiltration 10–15 km to the noilh and south of the ventfield in 1991, and insignificant infiltration at stations 50kmto the west of the vent field in 1992. A bootstrap analysiscomparing the faunal composition of nets towed above 900 m depthshowed that shallow fauna were not significantly distinct betweenthe two sampling years or up to 50 km away from the vent field.     

Biosynthesis of Leishmania lipophosphoglycan: solubilization and partial characterization of the initiating mannosyiphosphoryltransferase

Lipophosphoglycan (LPG) is the predominant surface glycoconjugateof Leishmania promastigotes and consists of a capped polymerof Gal(ß1,4)Man(     

A defect in synapsis causes male sterility in a T-DNA-tagged Arabidopsis thaliana mutant

Fluorescence microscopy was used to study meiosis in microsporocytes from wild-type Arabidopsis thaliana and a T-DNA-tagged meiotic mutant. Techniques for visualizing chromosomes and β-tubulin in other plant species were evaluated and modified in order to develop a method for analyzing meiosis in A. thaliana anthers. Like most dicots, A. thaliana microsporocytes undergo simultaneous cytokinesis in which both meiotic divisions are completed prior to cytokinesis. However, two unique events were observed in wild-type A. thaliana that have not been reported in other angiosperms: (1) polarization of the microsporocyte cytoskeleton during prophase I prior to nuclear envelope breakdown, and (2) extensive depolymerization of microtubules just prior to metaphase II. The first observation could have implications regarding a previously uncharacterized mechanism for determining the axis of the metaphase I spindle during microsporogenesis. The second observation is peculiar since microtubules are known to be involved in chromosome alignment in other species; possible explanations will be discussed. A T-DNA-tagged meiotic mutant of A. thaliana ( syn1 ), which had previously been shown to produce abnormal microspores with variable DNA content, was also cytologically characterized. The first observable defect occurs in microsporocytes at telophase I, where some chromosomes are scattered throughout the cytoplasm, usually attached to stray microtubules. Subsequent developmental stages are affected, leading to complete male sterility. Based on similarities to synaptic mutants that have been described in other species, it is suggested that this mutant is defective in synaptonemal complex formation and/or cohesion between sister chromatids.     

Effects of electrically induced fatigue on the twitch and tetanus of paralyzed soleus muscle in humans

Shields, Richard K., Laura Frey Law, Brenda Reiling, KellySass, and Jason Wilwert. Effects of electrically induced fatigueon the twitch and tetanus of paralyzed soleus muscle in humans.J. Appl. Physiol. 82(5):1499-1507, 1997.We analyzed the twitch and summated torque(tetanus) during repetitive activation and recovery of the human soleusmuscle in individuals with spinal cord injury. Thirteen individualswith complete paralysis (9 chronic, 4 acute) had the tibial nerveactivated every 1,500 ms with a 20-Hz train (7 stimuli) for 300 ms anda single pulse at 1,100 ms. The stimulation protocol lasted 3 min andincluded 120 twitches and 120 tetani. Minimal changes were found forthe acute group. The chronic group showed a significant reduction inthe torque and a significant slowing of the contractile speeds of boththe twitch and tetanus. The decrease in the peak twitch torque was significantly greater than the decrease in the peak tetanus torque early during the fatigue protocol for the chronic group. The twitch time to peak and half relaxation time were prolonged during fatigue, which was associated with improved fusion of the tetanus torque. At theend of the fatigue protocol, the decrease in the peak twitch torque wasnot significantly different from the decrease in the peak tetanustorque. After 5 min of rest, the contractile speeds recovered causingthe tetanus to become unfused, but the tetanus torque became lessdepressed than the twitch torque. The differential responses for thetwitch and the tetanus suggest an interplay between optimal fusioncreated from contractile speed slowing and excitation contractioncoupling compromise. These issues make the optimal design of functionalelectrical stimulation systems a formidable task.

     

Electron microprobe analysis of juvenile walleye pollock,Theragra chalcogramma,otoliths from Alaska: a pilot stock separation study

Synopsis The incorporation of dissolved oceanic constituents in the otoliths of fish has potential as a chemical tracer for reconstructing the early life history of marine fish. Wavelength dispersive spectrometers on an electron microprobe were used to measure Na, Mg, P, S, Cl, K, Ca, and Sr concentrations on the outer margins of 57 juvenile walleye pollock, Theragra chalcogramma, otoliths from five locations in the Gulf of Alaska and Bering Sea. Discriminant analyses that used various combinations of Na, P, K, Sr, and fish standard length and/or age showed that 60–80% of the samples could be assigned to the correct capture locality. While the concentrations of some of the measured elements correlated with standard length or age of the fish, there are measurable differences among localities when concentrations are length or age corrected, mainly due to differences in Na and K concentrations. Elemental composition of otoliths potentially could be used to assign fish from a mixed stock fishery to original stocks, information that is greatly needed for the effective management of fish stocks.     

Localization and Substrate Specificity of Glycosidases in Vacuoles of Nicotiana rustica

Vacuoles isolated from Nicotiana rustica var brasilia have been shown to contain significant levels of glycosidase activity when assayed using p-nitrophenyl-glycosides as substrates. The substrate specificity for the glycosidases in the vacuolar fraction closely paralleled that found in the protoplasts, and the leaf tissue from which the vacuoles were isolated. The substrate specificity of the vacuolar enzyme(s) was different from glycosidic activity found in the commercial digestive enzyme preparations used to isolate the protoplasts from leaf tissue. It was demonstrated that 70 to 90% of the glycosidases that were found in the protoplasts appeared to be localized within the vacuole, when the p-nitrophenyl substrates α- and β-;d-galactose, β-d-glucose, and α-d-mannose were used. Neither the vacuolar nor the protoplast enzymes were active towards the naturally occurring phenolic glycoside, rutin. α-Mannosidase appears to be a valuable marker enzyme for vacuoles isolated from mesophyll leaf cells of tobacco.     

Localization of stomatogastric IV neuron cell bodies in lobster brain

Summary The cell bodies of the inferior ventricular nerve (IVN) through-fibers of the lobster stomatogastric nervous system were located using cobalt chloride backfills and intracellular recordings. Following backfills of the IVN, two cell bodies in the supraesophageal ganglion (or brain) were stained with cobalt. These cells, each approximately 30 m in diameter, were located at the base of the IVN, just inside the connective tissue sheath surrounding the brain, and were identifiable on the basis of their close proximity to the IVN.In order to record from the cells, an in vitro preparation was made which included the cell bodies, their axons in the IVN and the stomatogastric nervous system. Intracellular recordings showed that the axons projected to the stomatogastric ganglion and made synaptic connections onto identified neurons. The axon trajectories and synaptic connections correlated with those previously described for the IVN through-fibers using extracellular stimulation and recording techniques.Abbreviations IVN inferior ventricular nerve - SN stomatogastric nerve     

Reciprocal translocation and the Philadelphia chromosome

Summary We examined metaphases from three patients with chronic myeloid leukaemia and a typical Philadelphia chromosome with one chromosome 9 as the recipient to determine whether the 9q+ 22q- translocation is reciprocal. Good quality G-banded photographs of the chromosomes concerned were subjected to light absorption density analysis. This provided enlarged tracings corresponding to the relevant chromosome regions and so facilitated accurate measurement. This technique has unambiguously shown that the typical Philadelphia chromosome results from a reciprocal translocation and that probably no material is gained or lost in the exchange. Furthermore, in a total of six patients for whom sequential G and C banding was performed, the chromosome 9 with the largest block of centromeric heterochromatin received the translocated material. We offer tentative explanations for this curious observation.     

Inflammatory cells in solid murine neoplasms. IV. Cytolytic T lymphocytes isolated from regressing or progressing Moloney sarcomas.

Highly purified suspensions of intratumoral T lymphocytes, recovered 11 and 13 days after induction of regressing or progressing Moloney sarcomas, were compared in their ability to lyse specifically the MSC cells used for tumor induction. Cytolytic activity, expressed in terms of lytic units/10(6) T cells, was similar for intratumoral T cell suspensions obtained 11 days after induction of either regressing (3.1 +/- 1.3 LU/10(6) T cells) or progressing (4.3 +/- 1.8) neoplasms. By 13 days post-induction, regressing tumors contained T lymphocytes with an increased cytolytic activity (11.1 +/- 4.5) whereas those from progressing tumors were strikingly less able to kill MSC cells (less than or equal to 0.2). This dramatic loss in cytotoxicity could not be attributed to errors associated with the enzymatic disaggregation method, inhibition by copurified endogenous tumor cells, or immunosuppression induced by viral infection. The changes in functional activity of intratumoral T lymphocytes from the two types of sarcoma appeared to be correlated with the stage of neoplasia. In this model system, cytolytic activity of T lymphocytes increased during spontaneous tumor regression whereas losses in cytotoxicity occurred coincident with the onset of inexorable progression.