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Leonore Brecher 《Development genes and evolution》1922,50(1-2):209-308
Ohne ZusammenfassungEin Auszug dieser Arbeit erschien mit gleichlautendem Titel als Mitteilung Nr. 59 aus der Biologischen Versuchsanstalt der Akademie der Wissenschaften, Zoologische Abteilung, Vorstand H. Przibram, im Akademischen Sitzungsanzeiger Nr. 7 u. 8, 1921. 相似文献
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Biosynthesis of fibronectin by rabbit aorta 总被引:1,自引:0,他引:1
The in vitro interactions between vascular cells and fibronectin have been shown to influence phenotypic expression of both cultured endothelial and smooth muscle cells. To more effectively assess the potential functional role of fibronectin in vivo in modulating vascular phenotypes, we have established methodology for studying fibronectin biosynthesis in the rabbit aorta using aortic rings that are morphologically and functionally intact and metabolically active. Aortic rings were incubated with 35S-labeled methionine in a supplemented physiological salt solution. The tissue was fractionated, and quantitative immunoprecipitation was performed using a polyclonal antibody directed against human plasma fibronectin. Newly synthesized fibronectin was most abundant in the fraction solubilized using 4% sodium dodecyl sulfate and in the incubation medium. In all fractions studied, fibronectin was present predominantly as a dimer with no detectable aggregates of fibronectin. Pulse-chase experiments showed that a substantial amount of newly synthesized fibronectin was found in the 4% sodium dodecyl sulfate extract after only 1 h, suggesting that fibronectin was rapidly incorporated into the extracellular matrix. The more soluble forms of newly synthesized fibronectin appeared to be the precursors for secreted fibronectin, and no precursor-product relationship between soluble and insoluble fibronectin was found. Dissection of aortic rings following incubation with labeled methionine showed that newly synthesized fibronectin was uniformally distributed in both intima-media and media-adventitia segments. Endothelial cell denudation caused only a 20% decrease of fibronectin biosynthesis concomitant with similar changes in total protein biosynthesis, consistent with the medial smooth muscle cell as the major source of newly synthesized fibronectin. Biosynthesis of fibronectin was increased following a 24-h preincubation of the aortic rings, and concomitant increases in steady state mRNA for fibronectin were found. These in vitro studies documented the utility of aortic rings for the general purpose of studying protein synthesis in vascular cells and provide new information on the characteristics of fibronectin biosynthesis by aortic tissue. 相似文献
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P Brecher M Tabacchi H Y Pyun A V Chobanian 《Biochemical and biophysical research communications》1973,54(4):1511-1517
Adrenal cell suspensions obtained by collagenase digestion of rat adrenal capsules was demonstrated to bind tritiated angiotensin II. The binding was rapid and reversible and was temperature dependent. Saturation of binding sites of a low order of capacity could be demonstrated by the addition of unlabeled angiotensin II. Specificity for this binding was demonstrated using several peptide analogues. Specificity was also observed with respect to cell type. These studies suggest the presence of a biologically significant receptor for angiotensin in cells of the zona glomerulosa of rat adrenal glands. 相似文献
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Summary 1-Antitrypsin was isolated as an electrophoretically homogeneous protein in preparative quantities from malignant human breast tissue by a two-step procedure of affinity chromatography on Sepharose-chymotrypsin, followed by polyacrylamide gel electrophoresis. Additionally, 2-macroglobulin and 1-antichymotrypsin were bound to, and subsequently eluted from the column. Antithrombin III and 1-acid glycoprotein, which were also components of the tissue extracts, were not bound, and appeared in the breakthrough peak. 1-Antitrypsin appeared to be partially modified immunologically by Sepharose-chymotryppin chromatography. However, 2-macroglobulin remained immunologically unaltered. Upon subsequent polyacrylamide gel electrophoresis, the 1-antitrypsin regained its capacity to give an immunological reaction of complete identity. Inhibitory activity toward proteases is also observed in filtrates of 1000 molecular weight cut-off membrane filters. Endogenous caseinolytic activity was observed in several eluate fractions. However, only N-benzoyl-L-tyrosine-ethyl ester, of the model compounds tested, was cleaved. The esterase activity is attributed to a breast tissue component since the column was free from dissociable chymotrypsin. Affinity chromatography on Sepharose-chymotrypsin represents a means of rapidly separating 37.5% of the total antichymotryptic activity from breast tissue extracts in high purity, in a single step. 相似文献
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Binding of acyl-CoA to liver fatty acid binding protein: effect on acyl-CoA synthesis 总被引:5,自引:0,他引:5
The ability of purified rat liver and heart fatty acid binding proteins to bind oleoyl-CoA and modulate acyl-CoA synthesis by microsomal membranes was investigated. Using binding assays employing either Lipidex 1000 or multilamellar liposomes to sequester unbound ligand, rat liver but not rat heart fatty acid binding protein was shown to bind radiolabeled acyl CoA. Binding studies suggest that liver fatty acid binding protein has a single binding site acyl-CoA which is separate from the two binding sites for fatty acids. Experiments were then performed to determine how binding may influence acyl-CoA metabolism by liver microsomes or heart sarcoplasmic reticulum. Using liposomes as fatty acid donors, liver fatty acid binding protein stimulated acyl-CoA production, whereas that from heart did not stimulate production over control values. 14C-labeled fatty acid-fatty acid binding protein complexes were prepared, incubated with membranes, and acyl-CoA synthetase activity was determined. Up to 70% of the fatty acid could be converted to acyl-CoA in the presence of liver fatty acid binding protein but in the presence of heart fatty acid binding protein, only 45% of the fatty acid was converted. Liver but not heart fatty acid binding protein bound the acyl-CoA formed and removed it from the membranes. The amount of product formed was not changed by additional membrane, enzyme cofactors, or incubation time. Additional liver fatty acid binding protein was the only factor found that stimulated product formation. Acyl-CoA hydrolase activity was also shown in the absence of ATP and CoA. These studies suggest that liver fatty acid binding protein can increase the amount of acyl-CoA by binding this ligand, thereby removing it from the membrane and possibly aiding transport within the cell. 相似文献
100.
Effect of liver fatty acid binding protein on fatty acid movement between liposomes and rat liver microsomes. 总被引:3,自引:0,他引:3 下载免费PDF全文
Although movement of fatty acids between bilayers can occur spontaneously, it has been postulated that intracellular movement is facilitated by a class of proteins named fatty acid binding proteins (FABP). In this study we have incorporated long chain fatty acids into multilamellar liposomes made of phosphatidylcholine, incubated them with rat liver microsomes containing an active acyl-CoA synthetase, and measured formation of acyl-CoA in the absence or presence of FABP purified from rat liver. FABP increased about 2-fold the accumulation of acyl-CoA when liposomes were the fatty acid donor. Using fatty acid incorporated into liposomes made either of egg yolk lecithin or of dipalmitoylphosphatidylcholine, it was found that the temperature dependence of acyl-CoA accumulation in the presence of FABP correlated with both the physical state of phospholipid molecules in the liposomes and the binding of fatty acid to FABP, suggesting that fatty acid must first desorb from the liposomes before FABP can have an effect. An FABP-fatty acid complex incubated with microsomes, in the absence of liposomes, resulted in greater acyl-CoA formation than when liposomes were present, suggesting that desorption of fatty acid from the membrane is rate-limiting in the accumulation of acyl-CoA by this system. Finally, an equilibrium dialysis cell separating liposomes from microsomes on opposite sides of a Nuclepore filter was used to show that liver FABP was required for the movement and activation of fatty acid between the compartments. These studies show that liver FABP interacts with fatty acid that desorbs from phospholipid bilayers, and promotes movement to a membrane-bound enzyme, suggesting that FABP may act intracellularly by increasing net desorption of fatty acid from cell membranes. 相似文献