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111.
Casey B. Engstrom Breanna B. Raymond Joud Albeitshawish Anastasia Bogdanovic Lynne M. Quarmby 《Journal of phycology》2024,60(2):275-298
Thick-walled rosette-like snow algae were long thought to be a life stage of various other species of snow algae. Rosette-like cells have not been cultured, but by manually isolating cells from 38 field samples in southern British Columbia, we assigned a variety of rosette morphologies to DNA sequence. Phylogenetic analysis of Rubisco large-subunit (rbcL) gene, ribosomal internal transcribed spacer 2 (ITS2) rRNA region, and 18S rRNA gene revealed that the rosette-like cells form a new clade within the phylogroup Chloromonadinia. Based on these data, we designate a new genus, Rosetta, which comprises five novel species: R. castellata, R. floranivea, R. stellaria, R. rubriterra, and R. papavera. In a survey of 762 snow samples from British Columbia, we observed R. floranivea exclusively on snow overlying high-elevation glaciers, whereas R. castellata was observed at lower elevations, near the tree line. The other three species were rarely observed. Spherical red cells enveloped in a thin translucent sac were conspecific with Rosetta, possibly a developmental stage. These results highlight the unexplored diversity among snow algae and emphasize the utility of single-cell isolation to advance the centuries-old problem of disentangling life stages and cryptic species. 相似文献
112.
High abundance of a single taxon (amphipods) predicts aquatic macrophyte biodiversity in prairie wetlands 总被引:1,自引:0,他引:1
Larson Danelle M. DeJong Demey Anteau Michael J. Fitzpatrick Megan J. Keith Breanna Schilling Emily G. Thoele Barry 《Biodiversity and Conservation》2022,31(3):1073-1093
Biodiversity and Conservation - Conservation programs often aim to protect the abundance of individual species and biodiversity simultaneously. We quantified relations between amphipod densities... 相似文献
113.
114.
The forests of North America provide a variety of benefits including water, recreation, wildlife habitat, timber, and other forest products. However, they continue to face many biotic and abiotic stressors including fires, native and invasive pests, fragmentation, and air pollution. Forest health specialists have been monitoring the health of forests for many years. This paper highlights some of the most damaging forest stressors affecting North American forests in recent years and provides some projections of future risks. 相似文献
115.
Dhanuka P. Wasalathanthri Matthew S. Rehmann Yuanli Song Yan Gu Luo Mi Chun Shao Letha Chemmalil Jongchan Lee Sanchayita Ghose Michael C. Borys Julia Ding Zheng Jian Li 《Biotechnology and bioengineering》2020,117(10):3182-3198
Real-time monitoring of bioprocesses by the integration of analytics at critical unit operations is one of the paramount necessities for quality by design manufacturing and real-time release (RTR) of biopharmaceuticals. A well-defined process analytical technology (PAT) roadmap enables the monitoring of critical process parameters and quality attributes at appropriate unit operations to develop an analytical paradigm that is capable of providing real-time data. We believe a comprehensive PAT roadmap should entail not only integration of analytical tools into the bioprocess but also should address automated-data piping, analysis, aggregation, visualization, and smart utility of data for advanced-data analytics such as machine and deep learning for holistic process understanding. In this review, we discuss a broad spectrum of PAT technologies spanning from vibrational spectroscopy, multivariate data analysis, multiattribute chromatography, mass spectrometry, sensors, and automated-sampling technologies. We also provide insights, based on our experience in clinical and commercial manufacturing, into data automation, data visualization, and smart utility of data for advanced-analytics in PAT. This review is catered for a broad audience, including those new to the field to those well versed in applying these technologies. The article is also intended to give some insight into the strategies we have undertaken to implement PAT tools in biologics process development with the vision of realizing RTR testing in biomanufacturing and to meet regulatory expectations. 相似文献
116.
Wallace BK Foroutan S O'Donnell ME 《American journal of physiology. Cell physiology》2011,301(2):C316-C326
Increased blood-brain barrier (BBB) Na-K-Cl cotransporter activity appears to contribute to cerebral edema formation during ischemic stroke. We have shown previously that inhibition of BBB Na-K-Cl cotransporter activity reduces edema and infarct in the rat middle cerebral artery occlusion (MCAO) model of ischemic stroke. We have also shown that the BBB cotransporter is stimulated by the ischemic factors hypoxia, aglycemia, and arginine vasopressin (AVP), although the mechanisms responsible are not well understood. AMP-activated protein kinase (AMPK), a key mediator of cell responses to stress, can be activated by a variety of stresses, including ischemia, hypoxia, and aglycemia. Previous studies have shown that the AMPK inhibitor Compound C significantly reduces infarct in mouse MCAO. The present study was conducted to evaluate the possibility that AMPK participates in ischemic factor-induced stimulation of the BBB Na-K-Cl cotransporter. Cerebral microvascular endothelial cells (CMEC) were assessed for Na-K-Cl cotransporter activity as bumetanide-sensitive (86)Rb influx. AMPK activity was assessed by Western blot analysis and immunofluorescence methods using antibodies that detect total versus phosphorylated (activated) AMPK. We found that hypoxia (7% and 2% O(2)), aglycemia, AVP, and oxygen-glucose deprivation (5- to 120-min exposures) increase activation of AMPK. We also found that Compound C inhibition of AMPK reduces hypoxia-, aglycemia-, and AVP-induced stimulation of CMEC Na-K-Cl cotransporter activity. Confocal immunofluorescence of perfusion-fixed rat brain slices revealed the presence of AMPK, both total and phosphorylated kinase, in BBB in situ of both control and ischemic brain. These findings suggest that ischemic factor stimulation of the BBB Na-K-Cl cotransporter involves activation of AMPK. 相似文献
117.
Noise and complexity in human postural control: interpreting the different estimations of entropy 总被引:1,自引:0,他引:1
Background
Over the last two decades, various measures of entropy have been used to examine the complexity of human postural control. In general, entropy measures provide information regarding the health, stability and adaptability of the postural system that is not captured when using more traditional analytical techniques. The purpose of this study was to examine how noise, sampling frequency and time series length influence various measures of entropy when applied to human center of pressure (CoP) data, as well as in synthetic signals with known properties. Such a comparison is necessary to interpret data between and within studies that use different entropy measures, equipment, sampling frequencies or data collection durations.Methods and Findings
The complexity of synthetic signals with known properties and standing CoP data was calculated using Approximate Entropy (ApEn), Sample Entropy (SampEn) and Recurrence Quantification Analysis Entropy (RQAEn). All signals were examined at varying sampling frequencies and with varying amounts of added noise. Additionally, an increment time series of the original CoP data was examined to remove long-range correlations. Of the three measures examined, ApEn was the least robust to sampling frequency and noise manipulations. Additionally, increased noise led to an increase in SampEn, but a decrease in RQAEn. Thus, noise can yield inconsistent results between the various entropy measures. Finally, the differences between the entropy measures were minimized in the increment CoP data, suggesting that long-range correlations should be removed from CoP data prior to calculating entropy.Conclusions
The various algorithms typically used to quantify the complexity (entropy) of CoP may yield very different results, particularly when sampling frequency and noise are different. The results of this study are discussed within the context of the neural noise and loss of complexity hypotheses. 相似文献118.
Ying JingMichael Borys Samiksha NayakSusan Egan Yueming QianShih-Hsie Pan Zheng Jian Li 《Process Biochemistry》2012,47(1):69-75
The presence of aggregated forms of proteins can be problematic for therapeutics due to the potential for immunogenic and pharmacokinetic issues. Although downstream processing can remove the aggregated forms, inhibiting aggregate formation upstream during the cell culture stage could reduce the burden on downstream processing and potentially improve process yields. This study first examined the effects of environmental factors (temperature, pH, and dissolved oxygen) and medium components (bivalent copper ion, cysteine, and cystine) on the aggregation of two different recombinant fusion proteins expressed by Chinese hamster ovary (CHO) cells. Any strategy to reduce protein aggregation upstream during cell culture must also consider potential effects on critical upstream parameters such as cell growth, harvest titer, and protein sialylation levels. Manipulating the culture temperature shift and cystine concentration in the medium were both identified as effective and practical strategies for reducing protein aggregation in both CHO-cell expression systems. Furthermore, a combination of both strategies was more effective in reducing protein aggregation levels compared to either approach individually; and without any negative effects on harvest titer and protein sialylation. This study demonstrates a practical methodology for decreasing protein aggregation during upstream processing and emphasizes the importance of process understanding to ensure production of recombinant glycoprotein therapeutics with consistent product quality. 相似文献
119.
Ośmiałowski B 《Journal of molecular modeling》2012,18(4):1633-1644
Tautomerism in monomers/dimers and association of 2,5-dihydroxy-1,8-naphthyridine was studied at the DFT level recently recommended
for studies of non-covalent interactions. Studied dimers are stabilized by double and triple hydrogen-bonding. In some associates
the intermolecular proton transfer may take place. Transition state related to the double proton transfer reactions were calculated
and discussed in terms of energetics, changes in atomic charges upon association, aromaticity (HOMA), properties of hydrogen bond critical point (QTAIM methodology) and geometry change during this reaction. It was found that double proton transfer is supported by third
hydrogen bond or by weak secondary interaction. Some protons in transition states are shared between two basic atoms, while
other are covalently bound only to one of them. The said process leads to replacement of secondary interactions of attractive
character to repulsive and vice versa. Overall, results suggest that in subjected compound the triple hydrogen-bonded associate may be in equilibrium with double
hydrogen-bonded dimer. 相似文献
120.
Jan Matějů Štěpánka Říčanová Simona Poláková Michal Ambros Borys Kala Kristýna Matějů Lukáš Kratochvíl 《European Journal of Wildlife Research》2012,58(2):473-482
Reintroductions are considered an important part of the action plans and recovery strategies of endangered ground squirrel
species, but so far little is known about their proper methodology. We collected primary data on 12 European ground squirrel
reintroduction projects carried out at 14 localities in the Czech Republic, Slovakia and Poland since 1989. We focused on
seven methodological aspects of each reintroduction: selection of release site, method of releasing, date of releasing, origin
of released animals, total number of released animals, mean number of released animals per season and reintroduction site
management. The method of releasing was found to be the key factor in determining the settlement of animals at the target
locality. Only soft releasing methods, i.e. the use of enclosures and/or artificial burrows, ensure that animals remain at
the target locality. The other factors significantly determining reintroduction success are the number of released animals
per season (at least 23 animals required) and the total number of released animals (a minimum of 60 individuals). Long-term
management of the site and regular monitoring of the newly established population are necessary. Our recommendations, based
on experience with the successes and failures of previous reintroductions, could largely improve the efficiency of future
reintroductions of highly endangered species. 相似文献