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71.
I Vega-Naredo R Loureiro K A Mesquita I A Barbosa L C Tavares A F Branco J R Erickson J Holy E L Perkins R A Carvalho P J Oliveira 《Cell death and differentiation》2014,21(10):1560-1574
The relationship between mitochondrial metabolism and cell viability and differentiation in stem cells (SCs) remains poorly understood. In the present study, we compared mitochondrial physiology and metabolism between P19SCs before/after differentiation and present a unique fingerprint of the association between mitochondrial activity, cell differentiation and stemness. In comparison with their differentiated counterparts, pluripotency of P19SCs was correlated with a strong glycolytic profile and decreased mitochondrial biogenesis and complexity: round, low-polarized and inactive mitochondria with a closed permeability transition pore. This decreased mitochondrial capacity increased their resistance against dichloroacetate. Thus, stimulation of mitochondrial function by growing P19SCs in glutamine/pyruvate-containing medium reduced their glycolytic phenotype, induced loss of pluripotent potential, compromised differentiation and became P19SCs sensitive to dichloroacetate. Because of the central role of this type of SCs in teratocarcinoma development, our findings highlight the importance of mitochondrial metabolism in stemness, proliferation, differentiation and chemoresistance. In addition, the present work suggests the regulation of mitochondrial metabolism as a tool for inducing cell differentiation in stem line therapies.Embryonal carcinoma cells, including the P19 cell line, are pluripotent cancer stem cells (CSCs) derived from pluripotent germ cell tumors called teratocarcinomas. These have been described as the malignant counterparts of embryonic stem cells (ESCs) and are considered a good model to study stem cell (SC) differentiation. The P19 cell line can be maintained as undifferentiated cells (P19SCs) or differentiated (P19dCs) to any cell type of the three germ layers. Similar to ESCs, P19 cells differentiate with retinoic acid (RA) in a dose-dependent manner and depending on growth conditions.1 Although differentiation generally yields a mixed population of differentiated cells, P19 cells grown in monolayer and treated with 1 μM RA primarily differentiate in endoderm or mesoderm, while retaining their immortality.2, 3Although some therapeutic approaches for regenerative medicine and to targeting CSCs are based on differentiation4 and mitochondrial-targeted therapies,5, 6 very little is known about the role of mitochondrial metabolism in SC maintenance and differentiation.7 Several mitochondrial characteristics that distinguish transformed cells from healthy cells have been described,8 including increased mitochondrial transmembrane electric potential (Δψm), which may result from decreased mitochondrial ATP production under normoxia.9 Similarly, normal SCs primarily rely on glycolysis for energy supply, although the exact mechanism how this occurs in the presence of oxygen and the relationship between SC metabolism and cell fate control is not yet completely understood.10Given the mitochondrial involvement in stemness and differentiation,11 one can ask whether manipulation of mitochondrial physiology results in an improvement of therapy efficacy. Therefore, characterizing the metabolic and mitochondrial profiles of both SCs and differentiated cells holds promise in order to explain the resistance of cancer cells expressing an embryonic signature to mitochondrial-targeted therapies. In the present work, we have two tandem hypotheses: (a) metabolic and mitochondrial remodeling accompanies P19SC differentiation and (b) P19SC differentiation results in a higher susceptibility to mitochondrial-directed therapies. 相似文献
72.
Branco S 《Molecular ecology》2010,19(24):5566-5576
Serpentine soils impose physiological stresses that limit plant establishment and diversity. The degree to which serpentine soils entail constraints on other organisms is, however, poorly understood. Here, I investigate the effect of serpentine soils on ectomycorrhizal (ECM) fungi by conducting a reciprocal transplant experiment, where serpentine and nonserpentine ECM fungal communities were cultured in both their native and non-native soils. Contrary to expectation, serpentine soils hosted higher fungal richness compared to nonserpentine, and most species were recovered from serpentine soil, suggesting ECM fungi are not overall specialized or strongly affected by serpentine edaphic constraints. 相似文献
73.
Patrícia Branco António Candeias Ana Teresa Caldeira Marina González-Pérez 《Microbial biotechnology》2019,12(6):1237-1248
Dekkera bruxellensis is the main reason for spoilage in the wine industry. It renders the products unacceptable leading to large economic losses. Fluorescence In Situ Hybridization (FISH) technique has the potential for allowing its specific detection. Nevertheless, some experimental difficulties can be encountered when FISH technique is applied in the wine environment (e.g. matrix and cells’ autofluorescence, fluorophore inadequate selection and probes’ low specificity to the target organisms). An easy and fast in-suspension RNA-FISH procedure was applied for the first time for identifying D. bruxellensis in wine. A previously designed RNA-FISH probe to detect D. bruxellensis (26S D. brux.5.1) was used, and the matrix and cells’ fluorescence interferences, the influence of three fluorophores in FISH performance and the probe specificity were evaluated. The results revealed that to apply RNA-FISH technique in the wine environment, a red-emitting fluorophore should be used. Good probe performance and specificity were achieved with 25% of formamide. The resulting RNA-FISH protocol was applied in wine samples artificially inoculated with D. bruxellensis. This spoilage microorganism was detected in wine at cell densities lower than those associated with phenolic off-flavours. Thus, the RNA-FISH procedure described in this work represents an advancement to facilitate early detection of the most dangerous wine spoilage yeast and, consequently, to reduce the economic losses caused by this yeast to the wine industry. 相似文献
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Fifty-two stream segments were sampled from 16 August to 13 September in 1993 in the eastern Atlantic Rainforest of S?o Paulo
State, southeastern Brazil (22°55′–25°00′S, 44°48′–48°03′W). Forty-two macroalgal subgeneric taxa were found and the most
widespread species were Audouinella pygmaea (21% of sites), Compsopogon leptoclados and Microcoleus subtorulosus (19%). Macroalgal species number per sampling site ranged from 0 to six (2.6 ± 1.7) and was positively correlated to species
abundance, whereas species cover ranged from 0 to 70% of the stream bed (15.5 ± 20.8%). No significant correlation was found
among macroalgal species number and abundance with any physical or chemical variable analyzed. Most sites were dominated by
one or few macroalgal species, mainly, Audouinella macrospora, C. leptoclados and M. subtorulosus. No significant difference was found between the frequency distribution of variables measured for streams and for total macroalgae
but the most widespread species (A. pygmaea) differed significantly for current velocity, specific conductance, turbidity and pH. Overall means for macroalgal occurrence
include the following values: temperature (% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn%
hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x%
fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaamiwaaaa!36CA!\[X\] = 19.9°C), current velocity (% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn%
hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x%
fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaamiwaaaa!36CA!\[X\] = 45 cm s−1), oxygen saturation (% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr%
4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaamiwaaaa!36CA!\[X\]
= 66%), specific conductance (% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr%
4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaamiwaaaa!36CA!\[X\]
= 59.6 μS cm−1), turbidity (% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr%
4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaamiwaaaa!36CA!\[X\]
= 5 NTU) and pH (% MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr%
4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaamiwaaaa!36CA!\[X\]
= 7.1). This pattern of patchy distribution and dominance by few species has been suggested as typical of stream macroalgal
communities and has been ascribed to the rapid fluctuation of physical and chemical conditions. Total macroalgal species richness
as well as mean species number per sampling site were considerably lower than found in similar studies of other regions. The
Intermediate Disturbance Hypothesis was applied to explain these results: the same factor (high precipitation) responsible
for the maintainance of the high species diversity in the surrounding forest can be, paradoxically, a constraint to the development
of a more diverse macroalgal flora in streams. 相似文献
79.
The plankton community, chlorophyll-a, heterotrophic bacteria and physical and chemical features of the Paranoá Reservoir were studied at monthly intervals at seven stations from March 1988 to March 1989. Thermal structure had a circulation period from May to July and stratification during the other months. The phytoplankton consisted of 76 taxa, was dominated by the cyanophyte Cylindrospermopsis raciborskii and attained concentrations ranging from 7,759,000 up to 98,160,000 org. l–1. The zooplankton consisted of 36 taxa and was present in densities between 8 and 8,056 org. l–1. In stations, or seasons with highly eutrophic conditions, there was a decrease in total phytoplankton and an increase in bacteria and total zooplankton. The results had spatial and temporal variations. Spatial variation demonstrated the existence of water quality deterioration at two or three sampling points. Temporal variation showed the influences of water column stability and the dry versus rainy seasons on nutrient concentrations and the planktonic community. 相似文献
80.