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B Guhathakurta M Majumdar A K Sen D Sasmal A K Mukherjee A Datta 《Journal of general microbiology》1986,132(6):1641-1646
D-Glucuronic acid and D-glucosamine have an immunodominant role in the lipopolysaccharide (LPS) O-antigen of both the Ogawa and the Inaba subtypes of Vibrio cholerae O1. This was evident from the pronounced inhibitory effect on the LPS precipitin reaction demonstrated by these monosaccharides and by oligosaccharides containing either of them which were isolated from LPS hydrolysate. There was a considerable decrease in the antibody-combining capacity of chemically modified LPS in which the carboxyl group of the glucuronic acid had been reduced. Similarly, on deamination, the O-specific polysaccharide fraction of the LPS molecule from both subtypes completely lost the ability to precipitate the LPS antibody. 相似文献
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Abstract Adhesive capacity to human buccal cells (HBC) of Vibrio cholerae 01 was expressed optimally in synthetic culture medium. Bacterial adhesion was inhibited efficiently by fetuin and weakly by D -mannose, D -glucose and thyroglobulin. Pretreatment of HBC with neuraminidase, protease or trypsin reduced the adhesion of V. cholerae 01 cells. 相似文献
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B. Guhathakurta D. Sasmal S. Pal S. Chakraborty G.B. Nair A. Datta 《FEMS microbiology letters》1999,179(2):401-407
The presence of three major virulence genes toxR, tcpA and ctxA as well as expression of several putative virulence factors were compared in 12 Vibrio cholerae O139 and non-O1,non-O139 strains of clinical and environmental origin. All the strains possessed the gene encoding the regulatory protein TOXR. None of the non-O1, non-O139 strains as well as one of the O139 environmental strains carried the genes for ctxA and tcpA. Statistically significant differences in hemagglutinin and hemolysin production were observed amongst the strains depending on the source of their isolation. Expression of extracellular enzymes such as protease, elastase, neuraminidase, phospholipase A and phospholipase C, however, did not vary significantly from the groups of strains isolated from different sources. 相似文献
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Asish K. Sen Amal K. Mukherjee Brakti Guhathakurta Archana Dutta Dipti Sasmal 《Carbohydrate research》1980,86(1):113-121
Five oligosaccharides were isolated in pure state from the lipopolysaccharide of Vibrio cholera, Inaba 569 B, and their structures were elucidated. More-detailed information regarding the partial structure of the lipopolysaccharide, containing glucose, mannose, glucuronic acid, 2-amino-2-deoxyglucose, d-glycero-l-manno-heptose, and d-glycero-l-gluco-heptose, was obtained through Smith degradation, chromium trioxide oxidation, and graded hydrolysis studies of the lipopolysaccharide and its derived products. 相似文献
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Dutta S Das S Guhathakurta S Sen B Sinha S Chatterjee A Ghosh S Ahmed S Ghosh S Usha R 《Cellular and molecular neurobiology》2007,27(8):1035-1047
Autism is a neurodevelopmental disorder with early manifestation. It is a multifactorial disorder and several susceptible
chromosomal regions for autism are identified through genome scan studies. The gene coding for glutamate receptor 6 (GluR6 or GRIK2) has been suggested as a candidate gene for autism based on its localization in the autism specific region on chromosome 6q21
and the involvement of receptor protein in cognitive functions like learning and memory. Despite its importance, so far no
studies have been carried out on possible involvement of GluR6 with autism in the Indian population. Therefore in the present study, we have performed genetic analysis of three markers
of GluR6 (SNP1: rs2227281, SNP2: rs2227283, SNP3: rs2235076) for possible association with autism through population, and family-based
(TDT and HHRR) approaches. DSM-IV criteria and CARS/ADI-R have been utilized for diagnosis. Genotyping analysis for the SNPs
has been carried out in 101 probands with autism spectrum disorder, 180 parents and 152 controls from different regions of
India. Since the minor allele frequency of SNP3 was too low, the association studies have been carried out only for SNP1 and
SNP2. Even though two earlier studies have shown association of these markers with autism, the present case–control and TDT,
as well as HHRR analyses have not demonstrated any biased transmission of alleles or haplotypes to the affected offspring.
Thus our results suggest that these markers of GluR6 are unlikely to be associated with autism in the Indian population. 相似文献
27.
Suneel Rallapalli Dillip Kumar Bishi Rama Shanker Verma Kotturathu Mammen Cherian Soma Guhathakurta 《Biotechnology letters》2009,31(12):1843-1850
Human mesenchymal stem cells (MSCs), with capacity to differentiate into adipocytes, osteoblasts and chondrocytes, offer potential
for the development of novel treatments. A critical question in MSCs biology is whether this cell population possesses a relatively
uniform differentiation capability or is comprised of distinct subsets of progenitors committed to differentiate in particular
pathways. To quantify the changes during growth of MSCs, we analyzed the mesenchymal phenotype and differentiation ability
using a multi-marker PCR with six primer sets specific for CD73, CD90, CD105, CD166, CD45 and β-actin allowing a gel-based
differential detection of the PCR products. To determine degree of variability of MSCs populations in terms of proliferation,
cell proliferation assays were performed on expanded MSCs up to the sixth passage. At each passage, the osteogenic and adipogenic
differentiation potentials of MSCs were verified by culture in inductive media. RT-PCR and cytochemical analysis revealed
that, despite the loss of multipotentiality during expansion, certain markers remain expressed, indicating that these markers
are unlikely to be reflective of the MSC’s true ‘stem cell’ nature. Our results suggest that decrease in the expression of
MSCs specific markers correlates with down-regulation of proliferation ability and differentiation efficiency of MSCs. 相似文献
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