全文获取类型
收费全文 | 4589篇 |
免费 | 484篇 |
国内免费 | 1篇 |
出版年
2022年 | 23篇 |
2021年 | 82篇 |
2020年 | 48篇 |
2019年 | 66篇 |
2018年 | 70篇 |
2017年 | 73篇 |
2016年 | 124篇 |
2015年 | 186篇 |
2014年 | 199篇 |
2013年 | 240篇 |
2012年 | 320篇 |
2011年 | 323篇 |
2010年 | 185篇 |
2009年 | 175篇 |
2008年 | 242篇 |
2007年 | 249篇 |
2006年 | 242篇 |
2005年 | 203篇 |
2004年 | 206篇 |
2003年 | 200篇 |
2002年 | 182篇 |
2001年 | 75篇 |
2000年 | 69篇 |
1999年 | 65篇 |
1998年 | 95篇 |
1997年 | 48篇 |
1996年 | 49篇 |
1995年 | 41篇 |
1994年 | 43篇 |
1993年 | 46篇 |
1992年 | 57篇 |
1991年 | 68篇 |
1990年 | 43篇 |
1989年 | 52篇 |
1988年 | 46篇 |
1987年 | 41篇 |
1986年 | 46篇 |
1985年 | 37篇 |
1984年 | 44篇 |
1983年 | 31篇 |
1982年 | 41篇 |
1981年 | 25篇 |
1980年 | 26篇 |
1979年 | 19篇 |
1978年 | 21篇 |
1977年 | 20篇 |
1975年 | 23篇 |
1974年 | 33篇 |
1973年 | 20篇 |
1966年 | 21篇 |
排序方式: 共有5074条查询结果,搜索用时 15 毫秒
11.
12.
There are too many kinds of organisms to be able to study and manage each, yet the loss of a single species can sometimes unravel an ecosystem. Such `fusewire species'– critical in the same sense that an electrical fuse can cut out a whole circuit – would be a rewarding focus for research and management effort. However, this approach can only be effective if these `fusewires' represent but a small proportion of the number of species in the system.
To demonstrate methods for measuring what proportion of the species in a system are critical to ecosystem function.
The prevalence of fusewire species was measured in manipulative experiments on an aquatic microcosm.
No single genus deletion caused changes in key characteristics of the system.
Comparison of these results with other published studies shows that the proportion of critical fusewire species varies amongst different ecosystems. The oxidation pond microcosms were shown to contain no single species indispensable to system function. They appear to be ill-suited to a management strategy which focuses on priority eukaryote species. However, a single study provides no evidence that this result is general or even typical of other kinds of ecosystems; it is presented here as an empirical model. Other methods of investigation are available; they are less experimentally rigorous but more practical. These could provide important guidance in planning an approach to management in a particular ecosystem. 相似文献
Aim
To demonstrate methods for measuring what proportion of the species in a system are critical to ecosystem function.
Methods
The prevalence of fusewire species was measured in manipulative experiments on an aquatic microcosm.
Results
No single genus deletion caused changes in key characteristics of the system.
Main conclusions
Comparison of these results with other published studies shows that the proportion of critical fusewire species varies amongst different ecosystems. The oxidation pond microcosms were shown to contain no single species indispensable to system function. They appear to be ill-suited to a management strategy which focuses on priority eukaryote species. However, a single study provides no evidence that this result is general or even typical of other kinds of ecosystems; it is presented here as an empirical model. Other methods of investigation are available; they are less experimentally rigorous but more practical. These could provide important guidance in planning an approach to management in a particular ecosystem. 相似文献
13.
Reactive sulfhydryl groups of alpha 39, a guanine nucleotide-binding protein from brain. Location and function 总被引:2,自引:0,他引:2
J W Winslow J D Bradley J A Smith E J Neer 《The Journal of biological chemistry》1987,262(10):4501-4507
The guanine nucleotide-binding proteins which mediate hormonal inhibition of adenylate cyclase as well as hormonal regulation of other membrane functions are alpha, beta, and gamma heterotrimers which are structurally homologous to each other. In brain, the predominant guanine nucleotide-binding component is a 39-kDa protein whose physiological role is as yet unknown. We have used N-ethylmaleimide to define functionally important sulfhydryl groups on alpha 39. Three cysteine residues in the molecule are reactive in unliganded alpha 39. Alkylation of two of these is reduced when guanosine 5'-(3'-O-thio)triphosphate (GTP gamma S) is bound. We have isolated and sequenced tryptic peptides containing the three reactive cysteines. The octapeptide containing the GTP gamma S-insensitive cysteine is at a position equivalent to amino acids 106-113 of the transducin alpha subunit (Lochrie, M. A., Hurley, J. B., and Simon, M. I. (1985) Science 228, 96-99). However, the equivalent peptide in transducin does not contain a cysteine residue. Alkylation of this cysteine blocks ADP-ribosylation of cysteine 351 by pertussis toxin. However, alkylation does not prevent association of alpha with the beta X gamma subunits nor does it inhibit GTPase activity. The two GTP gamma S-sensitive cysteines are at positions equivalent to cysteines 139 and 286 of the transducin alpha subunit. Alkylation of these residues inhibits GTPase activity. Neither of these GTP gamma S-sensitive cysteines are in those regions of alpha 39 which are highly homologous to the GTP-binding site of elongation factor Tu (Jurnak, F. (1985) Science 230, 32-36). However, both are present in the brain 41-kDa guanine nucleotide-binding protein and in the two transducins. The conservation of these cysteine residues suggests that they are important for the function of the subunits. 相似文献
14.
Homology of pCS1 Plasmid Sequences with Chromosomal DNA in Clavibacter michiganense subsp. sepedonicum: Evidence for the Presence of a Repeated Sequence and Plasmid Integration 总被引:2,自引:1,他引:1
下载免费PDF全文
![点击此处可从《Applied microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Restriction fragments of pCS1, a 50.6-kilobase (kb) plasmid present in many strains of Clavibacter michiganense subsp. sepedonicum (“Corynebacterium sepedonicum”), have been cloned in an M13mp11 phage vector. Radiolabeled forms of these cloned fragments have been used as Southern hybridization probes for the presence of plasmid sequences in chromosomal DNA of this organism. These studies have shown that all tested strains lacking the covalently closed circular form of pCS1 contain the plasmid in integrated form. In each case the site of integration exists on a single plasmid restriction fragment with a size of 5.1 kb. Southern hybridizations with these probes have also revealed the existence of a major repeated sequence in C. michiganense subsp. sepedonicum. Hybridizations of chromosomal DNA with deletion subclones of a 2.9-kb plasmid fragment containing the repeated sequence indicate that the size of the repeated sequence is approximately 1.3 kb. One of the copies of the repeated sequence is on the plasmid fragment containing the site of integration. 相似文献
15.
Harry Holthöfer M.D. Ph.D. Bradley A. Schulte Samuel S. Spicer 《Cell and tissue research》1987,249(3):481-485
Summary To identify precisely the structural and functional cell type in the collecting duct of the rat kidney expressing binding sites for Dolichos biflorus agglutinin (DBA), we stained serial paraffin sections of kidney with horseradish peroxidase-labeled DBA and with immunocytochemical methods for localizing (Na++K+)-ATPase and carbonic anhydrase II (CA II), enzymes found preferentially in principal and intercalated cells, respectively. Most principal cells expressing a strong basolateral staining for (Na+ + K+)-ATPase showed binding sites for DBA at their luminal surfaces. However, a minority of cells rich in CA II and showing morphologic characteristics of intercalated cells also expressed DBA binding sites at their luminal surface and apical cytoplasm. These data suggest that DBA cytochemistrycan provide a useful tool for studying the functional polarity of the main cell types of the collecting duct of the rat kidney. 相似文献
16.
Summary A rapid, sensitive, and reproducible enzyme-linked immunosorbent assay (ELISA), for the detection of the serological sex-specific (Sxs) antigen (formerly termed H-Y antigen; see Introduction), is described. This assay uses bovine lestes extract as the solid phase antigen, and high-titer anti-Sxs antisera and a urease-conjugated anti rat-IgG as the first and second antibody respectively. The urea containing substrate causes a pH shift in a positive reaction, which in turn is visualized by the use of bromocresol purple as a pH indicator. The method, and some representative applications of it, are described in detail. 相似文献
17.
Charlene P. Wight Andrew J. Daugulis Reginald H. Lau Bradley N. White 《Applied microbiology and biotechnology》1989,31(4):338-341
Summary
Bacillus brevis 47 was cultivated in 2-1 fermentors to study the effect of medium supplementation on extracellular protein production. Additional polypeptone, when supplied initially or at 12 h (late exponential phase), had little stimulatory effect on extracellular protein levels, which reached 6–7 g/l after 48h. A large increase in protein production was observed, however, when polypeptone was added at 21 h (stationary phase). This addition resulted in the accumulation in the medium of 14 g/l protein after 48 h, and a total of 16 g/l when cell-bound protein was included. In all cases, glucose was consumed only very slowly. 相似文献
18.
Summary The apical surface coat of type II pneumocytes and Clara cells in pre- and post-natal rat lung was examined with lectin histochemical methods. Lectins fromHelix pomatia (HPA), peanut (PNA) andMaclura pomifera (MPA) were conjugated with horseradish peroxidase and used to stain paraffin sections of fixed lung with or without certain pre-treatments. HPA and MPA were observed to react with almost all type II pneumocytes at postnatal day 1. Type II pneumocytes that stained with a sialidase—PNA sequence increased from a few positive cells at postnatal day 5 to many in the adult. It has been reported that the surface coat of type II pneumocytes closely resembles that of Clara cells in its staining with histochemical methods employing cationic dyes or lectins including MPA and PNA. However, staining with HPA, especially after periodic acid oxidation, revealed many type II pneumocytes with strong reactivity but showed only a few Clara cells that were faintly positive. HPA also stained alveolar macrophages. The HPA affinity of macrophages, however, was labile to oxidation with periodic acid or galactose oxidase unlike that of type II pneumocytes. This difference suggests that HPA recognizes more than one type of sugar structure.To whom all correspondence and reprint requests should be addressed. 相似文献
19.
Bradley J. Pusey 《Environmental Biology of Fishes》1990,29(1):15-26
Synopsis Changes in the age/length structure of Lepidogalaxias salamandroides populations in temporary waters of south-western Australia were monitored over a 51 month period by regular field sampling. Each year the study area experienced a summer drought of approximately five months duration. During the drought period Lepidogalaxias burrows into the mud and aestivates and substantial mortality occurs during this period. Body lipid reserves decrease during this period suggesting that they are the main energy source used during aestivation. The amount of lipid remaining after aestivation appears to be important in determining when female fish reproduce. Males die after reproduction and achieve a maximum age of only about 12 months. Some females also reproduce and die at this age while others defer reproduction for another year. Fecundity is much higher in these older, larger fish: they must however, survive two drought periods before they reproduce. The evolution of the life history style shown by Lepidogalaxias salamandroides is discussed with respect to the well defined seasonality of the region and it is suggested that selection for reproduction at an early age is balanced by the constraints of offspring survival during the aestivation period. 相似文献
20.