全文获取类型
收费全文 | 610篇 |
免费 | 66篇 |
出版年
2021年 | 15篇 |
2019年 | 6篇 |
2017年 | 9篇 |
2016年 | 11篇 |
2015年 | 19篇 |
2014年 | 18篇 |
2013年 | 18篇 |
2012年 | 21篇 |
2011年 | 25篇 |
2010年 | 10篇 |
2009年 | 13篇 |
2008年 | 13篇 |
2007年 | 20篇 |
2006年 | 23篇 |
2005年 | 17篇 |
2004年 | 18篇 |
2003年 | 23篇 |
2002年 | 13篇 |
2001年 | 24篇 |
2000年 | 23篇 |
1999年 | 10篇 |
1998年 | 16篇 |
1997年 | 9篇 |
1996年 | 6篇 |
1993年 | 5篇 |
1992年 | 14篇 |
1991年 | 9篇 |
1990年 | 11篇 |
1989年 | 7篇 |
1988年 | 12篇 |
1987年 | 12篇 |
1986年 | 9篇 |
1985年 | 7篇 |
1984年 | 21篇 |
1983年 | 8篇 |
1982年 | 5篇 |
1981年 | 6篇 |
1980年 | 5篇 |
1979年 | 8篇 |
1978年 | 15篇 |
1977年 | 19篇 |
1976年 | 18篇 |
1975年 | 11篇 |
1974年 | 5篇 |
1973年 | 7篇 |
1972年 | 15篇 |
1971年 | 9篇 |
1970年 | 8篇 |
1968年 | 8篇 |
1899年 | 5篇 |
排序方式: 共有676条查询结果,搜索用时 15 毫秒
101.
102.
103.
104.
High-resolution NMR is used to study two series of benzyl-L - or D -glutamate: benzyl-L -aspartate random copolymers. The helix sense of the L -aspartate residues determined from the αCH and NH chemical shifts agrees with that obtained from ORD. The stability of the helical copolymers to TFA addition shows a minimum at the composition of helix-sense inversion for the L -glutamate:L -aspartate copolymers but no minimum for the lefthanded D -glutamate: L -aspartate series. The helix-coil transition of the glutamate and aspartate residues in each polymer is compared. For the D -glutamate: L -aspartate series no differences are found, indicating random copolymerization. In the L -glutamate:L -aspartate series significant differences between the transition midpoints are interpreted as resulting from irregular distribution of component residues along the chain. 相似文献
105.
106.
107.
The dissociation of chicken erythrocyte deoxyribonucleoprotein and some properties of its partial nucleoproteins 总被引:5,自引:0,他引:5
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
K. Murray E. M. Bradbury C. Crane-Robinson R. M. Stephens A. J. Haydon A. R. Peacocke 《The Biochemical journal》1970,120(4):859-871
Histones were completely dissociated from their native complex with DNA in 2.0m-sodium chloride. Histone fractions IIb, V and I were dissociated in 1.2m-sodium chloride, fractions V and I in 0.7m-sodium chloride and fraction I in 0.45m-sodium chloride. Repeated extraction of partial dRNP (deoxyribonucleoprotein) preparations with sodium chloride of the same concentration as that from which they were prepared resulted in release of histones that previously had remained associated with the DNA of the complex. Gradual removal of histones from dRNP was paralleled by an improvement in solubility, a decrease in wavelength of the u.v.-absorption minimum, and a fall in sedimentation coefficient of the remaining partial dRNP. X-ray diffraction patterns of partial dRNP preparations showed that removal of histone fractions I and V from dRNP did not destroy the super-coil structure of the dRNP, but further removal of histones did. Infrared spectra of partial dRNP preparations showed that in native dRNP histone fraction I was present in the form of extended, isolated polypeptide chains, and that the other histone fractions probably contain a helical component that lies roughly parallel to the polynucleotide chains in the double helix and an extended polypeptide component that is more nearly parallel to the DNA helix axis. An analysis of the sedimentation of partial dRNP preparations on sucrose gradients showed that native dRNP consists of DNA molecules each complexed with histone fractions of all types. 相似文献
108.
The Fine Structure of the Adipose Cell of the Leech Glossiphonia complanata 总被引:2,自引:1,他引:1
下载免费PDF全文
![点击此处可从《The Journal of cell biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The two distinct types of cytoplasm seen with the light microscope in the adipose cell of the leech Glossiphonia complanata have been identified in the electron microscope image of this cell. One of these, the basophil cytoplasm, contains many well oriented, paired membranes which are much more clearly evident when calcium ions are added to the fixative. The membranes sometimes appear as concentric arrays of lamellae and are thought to represent sections through a phospholipide-containing body. The paired membranes and the concentric lamellae have granules attached to them and resemble in size and structure the membranes of the endoplasmic reticulum encountered in many mammalian cells. Small dense cytoplasmic particles are present throughout the cell; they may be ferritin molecules, derived from the breakdown of haemoglobin taken in as food. On the basis of a previous histochemical study and the present electron microscope investigation, it is suggested that these paired membranes are similar to the organized type of mammalian ER and the results seem to confirm the belief that these membranes are composed of layers of phospholipoprotein together with attached particles of ribonucleoprotein. 相似文献
109.
110.
Mario A. Cepeda Jacob JH. Pelling Caitlin L. Evered Hon S. Leong Sashko Damjanovski 《Experimental cell research》2017,350(1):169-183
Membrane-type-1 Matrix Metalloproteinase (MT1-MMP) is a multifunctional protease that regulates ECM degradation, proMMP-2 activation, and varied cellular processes including migration and viability. MT1-MMP is believed to be a central mediator of tumourigenesis whose role is dictated by its functionally distinct protein domains. Both the localization and signal transduction capabilities of MT1-MMP are dependent on its cytoplasmic domain, exemplifying diverse regulatory functions. To further our understanding of the multifunctional contributions of MT1-MMP to cellular processes, we overexpressed cytoplasmic domain altered constructs in MCF-7 breast cancer cells and analyzed migration and viability in 2D culture conditions, morphology in 3D Matrigel culture, and tumorigenic ability in vivo. We found that the cytoplasmic domain was not needed for MT1-MMP mediated migration promotion, but was necessary to maintain viability during serum depravation in 2D culture. Similarly, during 3D Matrigel culture the cytoplasmic domain of MT1-MMP was not needed to initiate a protrusive phenotype, but was necessary to prevent colony blebbing when cells were serum deprived. We also tested in vivo tumorigenic potential to show that cells expressing cytoplasmic domain altered constructs demonstrated a reduced ability to vascularize tumours. These results suggest that the cytoplasmic domain regulates MT1-MMP function in a manner required for cell survival, but is dispensable for cell migration. 相似文献