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671.
672.
Summary The methods which have been used for the observation and assignment of resonances in the NMR spectra of proteins are reviewed. One such method, the selective deuteration of the aromatic protons of tryptophyl residues, is studied by NMR spectroscopy in model compounds in this paper, and in proteins in the following paper.On the basis of a reassignment of the PMR spectrum of the aromatic protons of L-tryptophan, the relative rates of H-D exchange in deutero-trifluoroacetic acid (d-TFA) are H-2 > H-5 > H-6 > H-4 – H-7. The energies of activation for the first order exchange of both the H-2 and H-5 protons is 12 k.cal.mol–1.The rate constant for exchange of the H-2 protons of tryptophyl residues in peptides is much greater than in the amino acid itself and 5–10 times that for exchange of the H-5 protons. This suggests that the method can be used to label tryptophyl residues in proteins rapidly and specifically.An invited article. 相似文献
673.
Background
Various statistical and machine learning methods have been successfully applied to the classification of DNA microarray data. Simple instance-based classifiers such as nearest neighbor (NN) approaches perform remarkably well in comparison to more complex models, and are currently experiencing a renaissance in the analysis of data sets from biology and biotechnology. While binary classification of microarray data has been extensively investigated, studies involving multiclass data are rare. The question remains open whether there exists a significant difference in performance between NN approaches and more complex multiclass methods. Comparative studies in this field commonly assess different models based on their classification accuracy only; however, this approach lacks the rigor needed to draw reliable conclusions and is inadequate for testing the null hypothesis of equal performance. Comparing novel classification models to existing approaches requires focusing on the significance of differences in performance. 相似文献674.
675.
Psychrophilic microorganisms and their cold-active enzymes 总被引:2,自引:0,他引:2
JE Brenchley 《Journal of industrial microbiology & biotechnology》1996,17(5-6):432-437
676.
677.
Cyclins are indispensable elements of the cell cycle and derangement of their function can lead to cancer formation. Recent studies have also revealed more mechanisms through which cyclins can express their oncogenic potential. This review focuses on the aberrant expression of G1/S cyclins and especially cyclin D and cyclin E; the pathways through which they lead to tumour formation and their involvement in different types of cancer. These elements indicate the mechanisms that could act as targets for cancer therapy. 相似文献
678.
679.
Three desert perennials from the Wahiba Sands, Oman were studied for their ability to withstrand high air temperatures combined with low water availability. Plants were also sprayed with antitranspirant to reduced water loss. This limited the potential for evaporative leaf cooling and resulted in high leaf temperatures in Prosopis cineraria (Druce) and Zygophyllum qatarense (Hadidi). Diurnal measurements were made of leaf and air temperature and of of photosynthesis and transpiration. Antitranspirants did not significantly reduce transpiration in Heliotropium kotschyi (Guerke), which contained high concentrations of the quaternary compound betaine. Transpiration continued through the hottest periods of the day and appeared to be essential for leaf cooling. All species appeared to have a threshold leaf temperature above which photosynthesis was severely impaired. Samples were taken for analysis of amino acids, quaternary ammonium compounds (QACs) and low molecular weight carbohydrates in conjunction photosynthesis and transpiration in order to examine any possible protective functions. In Prosopis cineraria the background concentration of the cyclitol, pinitol was high but there was no increase in response to the antitranspirant induced increase in leaf temperatures. Proline concentration increased in leaves of Zygophyllum qatarense throughout the day but this did not appear to be related to leaf temperature. 相似文献
680.
Enzyme-catalysed peptide amidation. Isolation of a stable intermediate formed by reaction of the amidating enzyme with an imino acid 总被引:3,自引:0,他引:3
A series of hydrazones and semicarbazones of glyoxylic acid were shown to have a potent inhibitory effect on the enzyme-catalysed conversion of D-Tyr-Val-Gly to D-Tyr-Val-NH2. Among the derivatives tested, the inhibitory activity was increased by the presence of hydrophobic substituents and decreased by polar substituents. The inhibition produced by glyoxylic acid phenylhydrazone was shown to be competitive. No inhibition was obtained with pyruvic acid phenylhydrazone, which possesses a methyl group in place of the alpha-H of glyoxylic acid phenylhydrazone. The inhibitory potencies of these non-peptide substances are in accord with the specificity exhibited by the amidating enzyme in its reaction with peptide substrates. The inhibition produced by the glyoxylic acid derivatives was shown to be due to their ability to act as substrates for the peptide-amidating enzyme. The product formed from [14C]glyoxylic acid phenylhydrazone was identified as oxalic acid phenylhydrazide by co-chromatography in three chromatographic systems. The results demonstrate that the enzyme-catalysed oxidation of glyoxylic acid phenylhydrazone takes place by a mechanism involving hydroxylation. It is implicit that peptide amidation catalysed by the same enzyme proceeds by a similar mechanism. 相似文献