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31.
Summary A monoclonal antibody against substance P was used for immunocytochemical staining of the central ganglia of the snail Helix aspersa and several peripheral tissues including the gut, reproductive system, cardiovascular system, tentacle and other muscles.Within the central ganglia many neurones, and many fibres in the neuropile and the nerves entering the ganglia, were stained for the SP-like material. The largest numbers of reactive cell bodies were in the pleural ganglia and on the dorsal surfaces of the pedal ganglia. A group of cells was also found, surrounding the right pedal-cerebral connective, that did not fluoresce, but were enveloped by reactive processes terminating directly onto the neurone somata.Specific staining was observed in all peripheral tissues examined and always appeared to be concentrated in nerve terminals. Most particularly these occurred in the heart and aorta, the pharyngeal retractor muscle and the tentacle. Although mostly present in muscular tissues, some fluorescence was also observed in the nervous layer surrounding the retina. The tentacular ganglion also contained immunoreactive cell bodies. 相似文献
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33.
Summary Bacteriophages for three strains of A. tumefaciens were concentrated by ultracentrifugation, stained with 1% phosphotungstic acid (PTA), or 0.5% uranyl acetate, and examined with the electron microscope. Phage PT11 was a bacillary-shaped particle with a whip-like tail containing a knob at its distal end. Phage PIIBNV6 appeared to have an icosahedral head. The wide non-contractile tail terminated in a plate with pegs. Phage PIIBNV6-C was an icosahedral particle with a short, spike-like tail. Host cells of A. tumefaciens were encapsulated rods bearing polar or lateral flagella.Published with approval of the Director, Wisconsin Agricultural Experiment Station, Madison, Wisconsin, U.S.A. 53706. 相似文献
34.
Summary The literature on the vacuolar structure of the human syncytiotrophoblast is briefly reviewed. Personal observations based on light and electron microscopic investigations are described and illustrated. The nature of the different kinds of syncytial vacuolation is discussed. In particular dilated vacuoles, which may be very large indeed, receive special attention; situated adjacent to the syncytial nuclei they have been called juxtanuclear vacuoles. They are directly continuous with the perinuclear spaces, and often receive tubular communications from the endoplasmic reticular system. The possible functional role of the syncytial vacuolar system is discussed in the light of the authors' findings and the related literature.Dedicated to Professor Dr. Kurt Goerttler on the occasion of his seventieth birthday. 相似文献
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36.
Reduced iron, B.P. 1932, an old form of medicinal metallic iron powder, was given by mouth to albino rats. Measurable toxic effects were not produced until the dose reached 10 g./kg. body weight, which is 10 times the LD50 of iron similarly given as ferrous sulfate. Death occurred at three days and after from doses of 60 to 100 g./kg. and was due to hemoconcentration and vascular congestion of the liver and kidneys resulting from absorption of iron through an inflamed gastrointestinal mucosa. Larger doses produced death in one to three days from bowel obstruction due to impaction of iron in the stomach and intestines. The results suggest that reduced iron is the least toxic of all iron medicinal preparations and that re-investigation of its therapeutic value is warranted. 相似文献
37.
Definition of subchromosomal intervals around the myotonic dystrophy gene region at 19q 总被引:18,自引:0,他引:18
D Schonk M Coerwinkel-Driessen I van Dalen F Oerlemans B Smeets J Schepens T Hulsebos D Cockburn Y Boyd M Davis 《Genomics》1989,4(3):384-396
The localization to 19q of the gene causing myotonic dystrophy (DM) has been defined more precisely by refinement of the physical location of several linked markers. A somatic cell hybrid mapping panel from cells with t(1;19), t(12;19), and t(X;19) translocation products was constructed to define five different intervals across 19q. In addition, we have derived a series of cell hybrids by irradiation of a der(19)-only hybrid to further subdivide the cen-q13.1 region. Using an array of 36 cloned genes, anonymous DNAs, and enzyme markers, we have tested the location of the panel breakpoints and refined the regional assignment of several of these markers. All markers tightly linked to DM are localized mainly within 19q13.2, thus suggesting that the DM gene is also close to this region. 相似文献
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39.
Identification and description of beta-structure in horse muscle acylphosphatase by nuclear magnetic resonance spectroscopy 总被引:1,自引:0,他引:1
V Saudek M R Wormald R J Williams J Boyd M Stefani G Ramponi 《Journal of molecular biology》1989,207(2):405-415
Nuclear magnetic resonance spectra of acylphosphatase were searched for signs of beta-structure, i.e. characteristic nuclear Overhauser enhancement patterns displayed in the two-dimensional spectra, typical chemical shifts, coupling constants and slow 2H-H exchange. The results provided identification of the main-chain resonances of amino acid residues involved in the beta-structure. The full sequential assignment of this region was gained by identification of some amino acid spin systems and their alignment with the primary sequence. The assignment of the side-chains was virtually completed subsequently and a list produced of nuclear magnetic resonance (n.m.r.) constraints derived from the spectra. The beta-structure consists of a beta-sheet with four antiparallel chains, one attached parallel chain, three tight turns and a beta-bulge. The conformation of the beta-sheet was determined by distance geometry calculation using the n.m.r. constraints (174 intraresidual, 107 sequential and 226 long-range distances, 32 torsion angles, phi, and 28 hydrogen bonds) as input. Observation of some interactions between the sheet and previously identified alpha-helical regions made it possible to give an outline of the three-dimensional structure of the enzyme. 相似文献
40.
Isolation of chromosome-21-specific DNA probes and their use in the analysis of nondisjunction in Down syndrome 总被引:8,自引:2,他引:6
Summary Thirteen single-copy, chromosome-21-specific DNA probes were isolated from a recombinant library made from flow-sorted chromosome 21 DNA and regionally mapped using a panel of somatic cell hybrids. Five probes mapped in the 21q21-q22.1 region, six to the 21q22.1-qter region, and one to each of the regions 21q22.1-q22.2 and 21q22.3. Two of these probes, one of which maps in the critical region for Down syndrome, have recently been shown to be expressed at high levels in Down syndrome brain tissue (Stefani et al. 1988). Following preliminary screening for restriction fragment lenght polymorphisms (RFLPs), five polymorphisms were discovered with four of the chromosome 21 DNA probes. A frequent MspI polymorphism detected by one of the probes was used in conjunction with four previously described polymorphic chromosome 21 probes to analyse the origin of nondisjunction in 33 families with a child or fetus with trisomy 21. The parental origin of the additional chromosome 21 was determined in 12 cases: in 9 (75%) of these it was derived from the mother and in the other 3 cases (25%) it was of paternal origin. Cytogenetic analysis of Q-banding heteromorphisms was informative in three of five families tested, and in each case the RFLP results were confirmed. The meiotic stage of nondisjunction was defined with confidence in five families, the results being obtained with pericentromeric RFLP or cytogenetic markers. Recombination between two nondisjoined chromosomes was demonstrated in one family and is consistent with the view that a lack of recombination between chromosome 21 homologues or failure of their conjunction is not the invariable cause of trisomy 21. 相似文献