全文获取类型
收费全文 | 750篇 |
免费 | 78篇 |
出版年
2018年 | 7篇 |
2017年 | 7篇 |
2016年 | 12篇 |
2015年 | 19篇 |
2014年 | 16篇 |
2013年 | 27篇 |
2012年 | 40篇 |
2011年 | 40篇 |
2010年 | 36篇 |
2009年 | 23篇 |
2008年 | 28篇 |
2007年 | 30篇 |
2006年 | 30篇 |
2005年 | 27篇 |
2004年 | 24篇 |
2003年 | 22篇 |
2002年 | 21篇 |
2001年 | 17篇 |
2000年 | 27篇 |
1999年 | 22篇 |
1998年 | 10篇 |
1997年 | 10篇 |
1995年 | 7篇 |
1993年 | 9篇 |
1992年 | 15篇 |
1991年 | 17篇 |
1990年 | 9篇 |
1989年 | 6篇 |
1988年 | 10篇 |
1987年 | 17篇 |
1986年 | 13篇 |
1985年 | 10篇 |
1984年 | 9篇 |
1983年 | 9篇 |
1982年 | 9篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1979年 | 6篇 |
1978年 | 13篇 |
1977年 | 19篇 |
1975年 | 8篇 |
1974年 | 9篇 |
1973年 | 12篇 |
1972年 | 10篇 |
1971年 | 10篇 |
1970年 | 8篇 |
1969年 | 10篇 |
1968年 | 6篇 |
1967年 | 10篇 |
1966年 | 8篇 |
排序方式: 共有828条查询结果,搜索用时 15 毫秒
101.
Valerie J McKenzie Robert M Bowers Noah Fierer Rob Knight Christian L Lauber 《The ISME journal》2012,6(3):588-596
Although all plant and animal species harbor microbial symbionts, we know surprisingly little about the specificity of microbial communities to their hosts. Few studies have compared the microbiomes of different species of animals, and fewer still have examined animals in the wild. We sampled four pond habitats in Colorado, USA, where multiple amphibian species were present. In total, 32 amphibian individuals were sampled from three different species including northern leopard frogs (Lithobates pipiens), western chorus frogs (Pseudacris triseriata) and tiger salamanders (Ambystoma tigrinum). We compared the diversity and composition of the bacterial communities on the skin of the collected individuals via barcoded pyrosequencing of the 16S rRNA gene. Dominant bacterial phyla included Acidobacteria, Actinobacteria, Bacteriodetes, Cyanobacteria, Firmicutes and Proteobacteria. In total, we found members of 18 bacterial phyla, comparable to the taxonomic diversity typically found on human skin. Levels of bacterial diversity varied strongly across species: L. pipiens had the highest diversity; A. tigrinum the lowest. Host species was a highly significant predictor of bacterial community similarity, and co-habitation within the same pond was not significant, highlighting that the skin-associated bacterial communities do not simply reflect those bacterial communities found in their surrounding environments. Innate species differences thus appear to regulate the structure of skin bacterial communities on amphibians. In light of recent discoveries that some bacteria on amphibian skin have antifungal activity, our finding suggests that host-specific bacteria may have a role in the species-specific resistance to fungal pathogens. 相似文献
102.
103.
The temporal stability and change of the dominant phylogenetic groups of the domain bacteria were studied in a model plant-based
industrial wastewater treatment system showing high levels of organic carbon removal supported by high levels of N2 fixation. Community profiles were obtained through terminal restriction fragment length polymorphism analysis and cloning
of 16S rRNA amplicons followed by sequencing. Bacterial community profiles showed that ten common terminal restriction fragments
made up approximately 50% of the measured bacterial community. As much as 42% of the measured bacterial community could be
monitored by using quantitative PCR and primers that targeted three dominant operational taxonomic units. Despite changes
in wastewater composition and dissolved oxygen levels, the bacterial community composition appeared stable and was dominated
by α-Proteobacteria and β-Proteobacteria, with a lesser amount of the highly diverse bacterial phylum Bacteroidetes. A short
period of considerable change in the bacterial community composition did not appear to affect treatment performance indicating
functional redundancy in this treatment system. 相似文献
104.
Advancing ecological research with ontologies 总被引:1,自引:0,他引:1
105.
A compilation of soybean ESTs: generation and analysis. 总被引:18,自引:0,他引:18
Randy Shoemaker Paul Keim Lila Vodkin Ernest Retzel Sandra W Clifton Robert Waterston David Smoller Virginia Coryell Anupama Khanna John Erpelding Xiaowu Gai Volker Brendel Christina Raph-Schmidt E G Shoop C J Vielweber Matt Schmatz Deana Pape Yvette Bowers Brenda Theising John Martin Michael Dante Todd Wylie Cheryl Granger 《Génome》2002,45(2):329-338
Whole-genome sequencing is fundamental to understanding the genetic composition of an organism. Given the size and complexity of the soybean genome, an alternative approach is targeted random-gene sequencing, which provides an immediate and productive method of gene discovery. In this study, more than 120000 soybean expressed sequence tags (ESTs) generated from more than 50 cDNA libraries were evaluated. These ESTs coalesced into 16928 contigs and 17336 singletons. On average, each contig was composed of 6 ESTs and spanned 788 bases. The average sequence length submitted to dbEST was 414 bases. Using only those libraries generating more than 800 ESTs each and only those contigs with 10 or more ESTs each, correlated patterns of gene expression among libraries and genes were discerned. Two-dimensional qualitative representations of contig and library similarities were generated based on expression profiles. Genes with similar expression patterns and, potentially, similar functions were identified. These studies provide a rich source of publicly available gene sequences as well as valuable insight into the structure, function, and evolution of a model crop legume genome. 相似文献
106.
107.
Effects of plant phenology, nutrients and herbivory on growth and defensive chemistry of plantain, Plantago lanceolata 总被引:1,自引:0,他引:1
To assess the combined effect of herbivory, nutrient availability and plant phenology on plant mass and defensive chemistry, we conducted a field experiment with plantain ( Plantago lanceolata : Plantaginaceae) using three levels of herbivory, three levels of fertilizer and two harvest dates. Shoot mass of the no-herbivory plants showed a nonlinear response to increased fertilizer such that mass with high fertilizer was no greater than that with low fertilizer. In contrast, shoot mass of the low-herbivory plants (12% damage) was not influenced by fertilizer, but for high-herbivory plants (23% damage), there was a positive linear response to increased fertilizer. Increasing nutrient levels caused a decrease in iridoid glycoside concentration. Herbivory did not induce higher iridoid glycoside concentration in leaves of any age. But increasing herbivory resulted in a decrease in the concentration of catalpol in new leaves. Another experiment assessed how leaf age and plant age affected plant defensive chemistry. Total iridoid glycosides increased over 5 weeks, but catalpol only increased in new leaves. Overall, the order of importance in determining variation in iridoid glycoside concentration was plant phenology, nutrient availability and, to a much lesser extent, herbivory. 相似文献
108.
Kjetill S Jakobsen Torstein Tengs Andreas Vatne Holly A Bowers David W Oldach JoAnn M Burkholder Howard B Glasgow Parke A Rublee Dag Klaveness 《Proceedings. Biological sciences / The Royal Society》2002,269(1487):211-214
Several dinoflagellate strains of the genus Pfiesteria were isolated by culturing techniques from sediment samples taken in the Oslofjord region of Norway. Pfiesteria piscicida, well known as a fish killer from the Atlantic coast of America, was identified by genetic methods and light microscopy. The related species Pfiesteria shumwayae was attracted from the sediment by the presence of fish, and has proved toxic. This present survey demonstrates the wide distribution of these potentially harmful species, but so far they have not been connected with fish kills in Europe. 相似文献
109.
110.
This review discusses the known features of diurnal vertical migration in the opossum shrimpMysis relicta. Field observations describe a noctural pattern which is correlated to changing patterns of illumination. Hypothesis that offer evolutionary origins of vertical migration are presented which link trophic interactions of the migrator to their predators and prey. These couplings are examined for the case ofMysis relicta. All of the hypotheses are partially supported byMysis behavior. 相似文献