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Donald T. McKnight Monal M. Lal Deborah S. Bower Lin Schwarzkopf Ross A. Alford Kyall R. Zenger 《Molecular ecology》2019,28(11):2731-2745
Recent decades have seen the emergence and spread of numerous infectious diseases, often with severe negative consequences for wildlife populations. Nevertheless, many populations survive the initial outbreaks, and even undergo recoveries. Unfortunately, the long‐term effects of these outbreaks on host population genetics are poorly understood; to increase this understanding, we examined the population genetics of two species of rainforest frogs (Litoria nannotis and Litoria serrata) that have largely recovered from a chytridiomycosis outbreak at two national parks in the Wet Tropics of northern Australia. At the wetter, northern park there was little evidence of decreased genetic diversity in either species, and all of the sampled sites had high minor allele frequencies (mean MAF = 0.230–0.235), high heterozygosity (0.318–0.325), and few monomorphic markers (1.4%–4.0%); however, some recovered L. nannotis populations had low Ne values (59.3–683.8) compared to populations that did not decline during the outbreak (1,537.4–1,756.5). At the drier, southern park, both species exhibited lower diversity (mean MAF = 0.084–0.180; heterozygosity = 0.126–0.257; monomorphic markers = 3.7%–43.5%; Ne = 18.4–676.1). The diversity patterns in this park matched habitat patterns, with both species having higher diversity levels and fewer closely related individuals at sites with higher quality habitat. These patterns were more pronounced for L. nannotis, which has lower dispersal rates than L. serrata. These results suggest that refugia with high quality habitat are important for retaining genetic diversity during disease outbreaks, and that gene flow following disease outbreaks is important for re‐establishing diversity in populations where it was reduced. 相似文献
95.
Mating system and inbreeding depression in quantitative traits of whitebark pine (Pinus albicaulis Engelm.) was determined using isozymes and a seedling common garden experiment. Simultaneous isozyme analysis of embryo and
haploid megagametophyes from progeny arrays of families in three distinct geographic regions (Oregon, Montana, and southern
British Columbia) was used to estimate parental and progeny inbreeding coefficients, as well as regional and family mean multilocus
outcrossing rates (t
m). Quantitative trait family means of seedlings from the same families growing in two temperature treatments in a common garden
experiment were regressed on the estimated inbreeding coefficient to determine the presence and magnitude of inbreeding depression.
Regional estimates of t
m ranged from 0.73 to 0.93, with a mean over all regions of 0.86. Family mean t
m values indicated predominant outcrossing; however, some individuals experienced substantial inbreeding. The Oregon region
had a significant excess of heterozygotes in the parental generation relative to Hardy–Weinberg equilibrium, while both the
Oregon and southern BC regions had a heterozygote deficiency in progeny, suggesting selection against inbred individuals.
Biomass in the ambient temperature treatment for the southern BC region was the only trait significantly related to inbreeding
coefficient. The mean inbreeding coefficient for this region was 0.25, and based on this relationship, mean predicted biomass
would be reduced by 19.6% in this region if inbred individuals are not removed by selection. The estimated outcrossing rate
of whitebark pine is slightly lower than most wind-pollinated conifers, and while most individuals are highly outcrossing,
some experience substantial inbreeding. 相似文献
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Olefin cross-metathesis (CM)-based protocols enable short, flexible and regiocontrolled access to substituted furan derivatives. Specifically, CM of allylic alcohol and enone components provides γ-hydroxyenone intermediates that are cycloaromatized to the final furan derivatives on exposure to either acid or a discrete Heck arylation step. This latter process concomitantly introduces an extra substituent onto the furan target with complete control of regiochemistry. The methodology described here serves as the basis for developing other CM-based entries to diverse heteroaromatic compounds. This protocol describes in detail the following stages of the furan procedures: (i) the tandem formation and acid-catalyzed cyclization of the γ-hydroxyenone to afford a 2,5-disubstituted furan directly; (ii) CM of an allylic alcohol with an enone to provide an isolated γ-hydroxyenone; and (iii) Heck arylation of this γ-hydroxyenone to afford a 2,3,5-trisubstituted furan. The reaction procedure given for the formation of the 2,5-disubstituted furan (option A) takes ~26.5 h to complete. The procedure described for the formation of the 2,3,5-trisubstituted furan (option B) takes ~52.5 h. 相似文献
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Simon van Mourik Aalt DJ van Dijk Maarten de Gee Richard GH Immink Kerstin Kaufmann Gerco C Angenent Roeland CHJ van Ham Jaap Molenaar 《BMC systems biology》2010,4(1):101
Background
The genetic control of floral organ specification is currently being investigated by various approaches, both experimentally and through modeling. Models and simulations have mostly involved boolean or related methods, and so far a quantitative, continuous-time approach has not been explored. 相似文献99.
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Momen A Bower D Boehmer J Kunselman AR Leuenberger UA Sinoway LI 《American journal of physiology. Heart and circulatory physiology》2004,287(6):H2834-H2839
During exercise, reflex renal vasoconstriction maintains blood pressure and helps in redistributing blood flow to the contracting muscle. Exercise intolerance in heart failure (HF) is thought to involve diminished perfusion in active muscle. We studied the temporal relationship between static handgrip (HG) and renal blood flow velocity (RBV; duplex ultrasound) in 10 HF and in 9 matched controls during 3 muscle contraction paradigms. Fatiguing HG (protocol 1) at 40% of maximum voluntary contraction led to a greater reduction in RBV in HF compared with controls (group main effect: P <0.05). The reduction in RBV early in HG tended to be more prominent during the early phases of protocol 1. Similar RBV was observed in the two groups during post-HG circulatory arrest (isolating muscle metaboreflex). Short bouts (15 s) of HG at graded intensities (protocol 2; engages muscle mechanoreflex and/or central command) led to greater reductions in RBV in HF than controls (P <0.03). Protocol 3, voluntary and involuntary biceps contraction (eliminates central command), led to similar increases in renal vasoconstriction in HF (n=4). Greater reductions in RBV were found in HF than in controls during the early phases of exercise. This effect was not likely due to a metaboreflex or central command. Thus our data suggest that muscle mechanoreflex activity is enhanced in HF and serves to vigorously vasoconstrict the kidney. We believe this compensatory mechanism helps preserve blood flow to exercising muscle in HF. 相似文献