台湾海峡的砂壳纤毛虫研究(纤毛动物门:砂壳亚目)

依据壳体形态对台湾海峡南部0－40m水层的40种砂壳纤毛虫进行了观察,发现其中有3种新种,即倪氏表纹虫Epirhabdonella niei,Xu,Hong et Song,sp.nov.,网状条纹虫Rhabdonella reticulata Xu,Hong et Song.sp.nov,和钝条纹虫Rhabdonella obtusa Xu,Hong et Song,sp.nov.及17个中国新纪录种,同时确定新村网纹虫Favella shintsuensis Nie et Cheng,1947为一同物异名。     

Visualization and solubilization of rat brain opiate receptors with a “κ” ligand selectivity pattern

1. Specific binding of [3H]ethylketocyclazocine (EkappaC), a prototype kappa-opiate agonist, to slide-mounted rat striatal sections is increased in the presence of 100 mM NaCl at 4 degrees C. 2. Under similar incubation conditions, binding of mu and delta prototype opiates is reduced to almost undetectable levels. 3. Correlation (P less than 0.01) of the ligand selectivity pattern of [3H]EKC displacement with the potencies of various opiate drugs in inhibiting the contractions of the rabbit vas deferens, a kappa-opiate receptor bioassay, suggests that the binding site under study represents the pharmacologically relevant kappa-opiate receptor. 4. Visualization of these kappa-opiate receptors with tritium-sensitive film reveals a striking, highly discrete brain distribution pattern (e.g., striatal patches, habenular stripe) which is similar to that of [3H]dihydromorphine and [3H]naloxone. 5. Soluble [3H]EKC binding sites obtained from rat membranes also possess a kappa-like ligand selectivity pattern, with bremazocine being a potent displacer while mu and delta ligands are almost inactive. 6. A possible explanation of these data is that the "kappa"-opiate binding site in rat brain is one transitional state of an opiate receptor capable of assuming distinct conformations with characteristic ligand selectivity patterns. Other possibilities such as pre and post-synaptic locations should also be considered.     

不同种植模式对烤烟根系和叶片衰老特性的影响

以烤烟品种K326为试材,采用大田试验研究了广东南雄地区不同种植模式（单行种植+前期垄体覆盖地膜、单行种植+前期垄体覆盖地膜+后期垄体覆盖稻草、双行凹垄种植+前期垄体覆盖地膜和双行凹垄种植+前期垄体覆盖地膜+后期垄体覆盖稻草）对烤烟根系和叶片衰老特性,以及烤后烟叶经济性状的影响.结果表明：与其他种植模式相比,双行凹垄种植+前期垄体覆盖地膜+后期垄体覆盖稻草处理显著促进了各时期烤烟根系生长,根系活力提高;叶片叶绿素含量和保护酶(SOD、POD)活性均较高,而MDA含量较低,烤后烟叶的产量、产值、均价和上等烟比例等经济性状均显著高于其他处理.双行凹垄种植+前期垄体覆盖地膜+后期垄体覆盖稻草处理可以改善烤烟根系和叶片衰老特性,提高烤后烟叶经济性状,是广东南雄地区优质烟叶生产的有效种植模式.     

Phycobilisomes in Blue-Green Algae

Fifteen species of freshwater blue-green algae, including unicellular, filamentous, and colonial forms, were subjected to a variety of fixatives, fixation conditions, and stains for comparison of the preservation of phycobilisomes. Absorption spectra of the corresponding in vivo and released photosynthetic pigments, in 10 of the species that were maintained in culture, demonstrated the presence of phycocyanin in all 10 species and phycoerythrin in only 2 of them. Spectroscope and electron microscope evidence was obtained for localization of phycobiliproteins in phycobilisomes of Nostoc muscorum. Phycobilisomes were observed in all species examined in situ, strenghening the hypothesis that phycobilisomes are common to all phycobiliprotein-containing photosynthetic blue-green algae.     

板蓝根热加工过程中氨基酸组分分析

利用氨基酸自动分析仪对板蓝根的新鲜植物组织和不同加工程度的板蓝根药材中的总氨基酸和游离氨基酸含量进行测定。结果表明:板蓝根的新鲜植物组织和不同加工程度的板蓝根药材氨基酸在含量上有明显差别。结论为:不同加工程度的板蓝根药材中氨基酸含量差别明显,其中碱性氨基酸含量的差别尤其显著,该差异可能是由于加工过程中发生美拉德反应造成的。     

The effects of methyltestosteone on reproductive function in male greyhounds

Oral administration of methyltestosterone (MT) at 50 mg/dog/day to intact adult male greyhounds for 90 d resulted in decreased (P < 0.05) mean daily sperm output and mean testicular length. Additionally, the mean diameter of seminiferous tubules in MT-treated dogs tended to decrease (P = 0.08). Mean concentrations of luteinizing hormone (LH) and follicle stimulating hormone (FSH) and concentrations of testosterone in serum were also decreased or tended to decrease (P = 0.0003 to 0.059) at various sampling periods during MT treatment, suggesting alterations in spermatogenesis resulted from decreased serum concentrations of gonadotropins and steroids. Mean daily sperm output, mean testicular length, mean seminiferous tubule diameter and mean concentrations of FSH in serum were not decreased (P > 0.05) at the end of a 90-d recovery period. However, mean concentrations of serum LH and concentrations of testosterone were still lower (P < 0.05) during five of six and one of six sampling times, respectively, during the recovery period than the pretreatment levels, suggesting a prolonged effect of MT treatment on the pituitary/gonadal axis.     

胃息肉与幽门螺杆菌感染关系研究

目的探讨胃息肉与幽门螺杆菌（Helicobacter pylori,H.pylori）感染关系。方法对1218例胃息肉同时进行H．pylori检查患者进行回顾性分析,分析胃息肉患者H.pylori感染率、胃息肉部位与H.pylori感染关系、胃息肉病理类型与H.pylori感染关系。结果发现胃息肉Hpylori感染患者532例,Hpylori感染率为43．7％。男性胃息肉患者H.pylori感染率为47．5％（216／455）,女性Hpriori感染率感染率为41．4％（316／763）（P〉0．05）,年龄〈20岁、20～39岁、40—59岁和≥60岁胃息肉H.priori感染率分别为41．7％、44．7％、41．6％和47．2％（P〉0．05）;胃窦胃角息肉H.pylori感染率高于其他部位（胃体、胃底和贲门）（P〈0．05）;炎性和增生性胃息肉H.priori感染率高于胃底腺和腺瘤性息肉（P〈0．05）。结论H.pylori感染可能与部分胃息肉发生有一定关系,需要进一步深入研究胃息肉的发生机制。     

临床常见L型细菌的分布及耐药性变化分析

目的了解临床主要L型致病菌的分布以及对目前常用性变化趋势.方法对2000年7月～2003年7月临床分离的753株药性进行回顾性分析.结果金黄色葡萄球菌、大肠埃希菌、最常见,它们共同对阿米卡星霉素敏感,敏感率达77%以上,共同对罗红霉素、氨苄西林、克林霉素、阿奇霉素耐药,耐药率均在45%以上.结论与我院5年前及10年前L型细菌耐药性对比,抗生素耐药性已发生变迁.掌握L型细菌耐药性的动态,合理使用抗生素.     

野生藏酋猴冬季食物组成及营养成分

觅食是获取营养物质和能量的重要途经。对于栖息在四季分明地区的灵长类动物而言,低温以及食物资源相对匮乏的冬季是其生存和生长发育的瓶颈期。本研究以安徽黄山的野生藏酋猴(Macaca thibetana)天湖山群为对象,于2019年11月至2020年1月采用瞬时扫描取样法(Instantaneous scan sampling)采集猴群觅食行为数据,并分析其冬季食物组成及食物中各化学成分含量对取食的影响。结果显示,野生藏酋猴在冬季共取食23科31属34种植物,主要包括壳斗科(Fagaceae, 21.62%)、樟科(Lauraceae, 17.57%)、蔷薇科(Rosaceae, 8.11%)的植物,取食部位以叶片(66.22%)和果实(种子)(24.32%)为主。不同取食部位的水分、总糖、淀粉、脂肪、单宁等成分存在显著差异。其中,叶片的水分含量高于果实(种子)、茎和芽,茎和果实(种子)含有较高的总糖,果实(种子)的淀粉和脂肪含量最高,芽的单宁含量最高。此外,取食植物中的总糖含量高于非取食植物。结果表明,野生藏酋猴适应寒冷冬季与食物匮乏的觅食策略是对植物种类、植物部位及其主要营养成分的综合结...