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101.
Calcium-induced alterations in mitochondrial morphology quantified in situ with optical scatter imaging 下载免费PDF全文
Optical scatter imaging (OSI), a technique we developed recently, was used to measure the ratio of wide-to-narrow angle scatter (OSIR) within endothelial cells subjected to calcium overload (1.6 mM) after permeabilization by ionomycin. Within a few minutes of calcium overload, the mitochondria, which started as elongated organelles, rounded up into spherically shaped particles. This change in morphology was accompanied by a statistically significant 14% increase in OSIR in the cells' cytoplasm. Mitochondrial rounding and OSIR increase were suppressed by cyclosporin A (25 microM), implying that the observed geometrical and scattering changes were directly attributable to the mitochondrial permeability transition. The angular scattering properties of a long mitochondrion rounding up were approximated by numerical simulations of light scatter from an ellipsoid rounding up into a sphere. The simulations predicted a relative increase in OSIR comparable to that measured experimentally for the case where the shape transition takes place with little or no volume increase. The simulations also suggested that mitochondrial refractive index changes could not account for the OSIR changes observed. Our data show that changes in OSIR correlate with mitochondrial morphology change in situ. OSI provides a new tool for subcellular imaging and complements other microscopy methods, such as fluorescence. 相似文献
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Suryapranata H Boland JL Pieper M Legrand VL Bonnier JJ Juliard JM Vrolix MC Seabra-Gomes R Hamburger JN Roguin A Oosterwijk C Van Es GA Beyar R Serruys PW 《International journal of cardiovascular interventions》2000,3(1):21-28
BACKGROUND: Although safety and efficacy of the beStent (Medtronic Inc., Santa Rosa, CA, USA) have been described, the long-term angiographic and clinical outcomes have yet to be investigated. The ROSE (Registry for Optimal beStent Evaluation) trial was designed to assess the procedural safety of single 15 mm beStent implantation, and the six-month angiographic and 12-month clinical outcomes of patients treated with this novel coronary stent. METHODS: Patients with angina and a single de novo lesion in a native coronary artery of >/=2.75 mm diameter were included in this multicenter, prospective, observational trial. Clinical follow-up was obtained at one, six and 12 months. Angiography was performed before and after the stent implantation and at six months. The primary end-point included major adverse cardiac events (death, myocardial infarction and target lesion revascularization), major bleeding complications, and thrombotic occlusions at one-month follow-up. Secondary end-points were major cardiac-event-free survival at six- and 12-month follow-up and angiographic restenosis at six months. A total of 120 patients (80% male, mean age 58.6 +/- 10.6 years) with stable (48%) or unstable (44%) angina pectoris were allocated. The target vessel reference diameter pre-procedure was 2.85 +/- 0.52 mm. RESULTS: Minimal lumen diameter pre/post and at follow-up was 0.97 +/- 0.28 mm, 2.53 +/- 0.40 mm and 1.86 +/- 0.63 mm, respectively. Restenosis rate according to the >50% diameter stenosis criterion at six-month follow-up was 21.5%. At 12 months, the event-free survival rate was 75% (no deaths, two Q-wave and seven non-Q-wave infarctions, five bypass surgery interventions and 16 target lesion revascularizations), whilst 87% of the patients were free of angina pectoris. CONCLUSION: Despite the relatively high percentage of small vessels, the outcome of the ROSE trial is comparable to those observed in previous stent trials, indicating that the coronary beStent is safe and effective as a primary device for the treatment of native coronary artery lesions in patients with (un)stable angina pectoris. 相似文献
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Courtney Michael B. Miller Emily A. Boustany Andre M. Van Houtan Kyle S. Catterson Matthew R. Pawluk Jason Nichols Jeff Seitz Andrew C. 《Environmental Biology of Fishes》2022,105(8):1081-1097
Environmental Biology of Fishes - Although steelhead (Oncorhynchus mykiss) is an iconic species found throughout the North Pacific rim, little is known about its ocean ecology. To provide insights... 相似文献
104.
Xianghai Ye Stewart R Brown Kátia Nones Luiz L Coutinho Jack CM Dekkers Susan J Lamont 《遗传、选种与进化》2007,39(1):73-89
Myostatin is a negative regulator of skeletal muscle growth. We evaluated effects of myostatin polymorphisms in three elite commercial broiler chicken lines on mortality, growth, feed conversion efficiency, ultrasound breast depth, breast percentage, eviscerated carcass weight, leg defects, blood oxygen level, and hen antibody titer to infectious bursal disease virus vaccine. Progeny mean data adjusted for fixed and mate effects and DNA from 100 sires per line were used. Single nucleotide polymorphisms (SNPs) of the myostatin gene segregating in these lines were identified by designing specific primers, amplifying individual DNA in each line by polymerase chain reaction, cloning, sequencing and aligning the corresponding products. Individual sires were genotyped for five identified SNPs which contributed to eight haplotypes. Frequencies of SNP alleles and haplotypes differed between lines. Using the allele substitution effect model, the myostatin SNPs were found to have significant (P < 0.031) associations with growth, mortality, blood oxygen and hen antibody titer to infectious bursal disease virus vaccine, although the associations were not often consistent across lines. These results suggest that the myostatin gene has pleiotropic effects on broiler performance. 相似文献
105.
Eddy J Smid Oylum Erkus Maciej Spus Judith CM Wolkers-Rooijackers Svetlana Alexeeva Michiel Kleerebezem 《Microbial cell factories》2014,13(Z1):S2
This review describes the recent advances made in the studies of the microbial community of complex and undefined cheese starter cultures. We report on work related to the composition of the cultures at the level of genetic lineages, on the presence and activity of bacteriophages and on the population dynamics during cheese making and during starter culture propagation. Furthermore, the link between starter composition and starter functionality will be discussed. Finally, recent advances in predictive metabolic modelling of the multi-strain cultures will be discussed in the context of microbe-microbe interactions. 相似文献
106.
Steven C. Lane Robert D. Jolly Donald E. Schmechel †Joseph Alroy Rose-Mary Boustany 《Journal of neurochemistry》1996,67(2):677-683
Abstract: Batten's disease is a genetic neurodegenerative disease of childhood. Its hallmarks are retinitis pigmentosa and neuronal degeneration. As some types of photoreceptor death in mice are mediated by apoptosis, we investigated whether apoptosis is responsible for retinal and neuronal degeneration in the late infantile and juvenile forms of Batten's disease. Using the terminal dUDP nick end-labeling (TUNEL) staining method, we detected apoptotic neuronal cells in brain from patients and a canine model and in brain and retina from an ovine model for Batten's disease. We confirmed apoptosis by flow cytometry, electron microscopy, and DNA laddering. This is the first inherited neurodegenerative disease involving brain and retina in which apoptosis has been established as the mechanism of neuronal and photoreceptor cell death in both humans and animal models. 相似文献
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Jonathan CM Clark Toru Akiyama Crispin R Dass Peter FM Choong 《Cancer cell international》2010,10(1):20