全文获取类型
收费全文 | 6303篇 |
免费 | 510篇 |
国内免费 | 2篇 |
专业分类
6815篇 |
出版年
2022年 | 46篇 |
2021年 | 71篇 |
2020年 | 35篇 |
2019年 | 69篇 |
2018年 | 62篇 |
2017年 | 85篇 |
2016年 | 145篇 |
2015年 | 197篇 |
2014年 | 243篇 |
2013年 | 369篇 |
2012年 | 394篇 |
2011年 | 402篇 |
2010年 | 284篇 |
2009年 | 299篇 |
2008年 | 396篇 |
2007年 | 411篇 |
2006年 | 398篇 |
2005年 | 387篇 |
2004年 | 391篇 |
2003年 | 412篇 |
2002年 | 384篇 |
2001年 | 72篇 |
2000年 | 54篇 |
1999年 | 88篇 |
1998年 | 133篇 |
1997年 | 86篇 |
1996年 | 72篇 |
1995年 | 84篇 |
1994年 | 65篇 |
1993年 | 57篇 |
1992年 | 64篇 |
1991年 | 50篇 |
1990年 | 38篇 |
1989年 | 34篇 |
1988年 | 33篇 |
1987年 | 22篇 |
1986年 | 22篇 |
1985年 | 27篇 |
1984年 | 37篇 |
1983年 | 16篇 |
1982年 | 32篇 |
1981年 | 28篇 |
1980年 | 33篇 |
1979年 | 30篇 |
1978年 | 19篇 |
1977年 | 20篇 |
1976年 | 19篇 |
1975年 | 18篇 |
1974年 | 15篇 |
1972年 | 15篇 |
排序方式: 共有6815条查询结果,搜索用时 15 毫秒
51.
Marie-Odile Parat Marie-Jeanne Richard Marie Th r se Leccia Pierre Amblard Alain Favier Jean Claude B ani 《Free radical research》1995,23(4):339-351
Reactive oxygen species (ROS) are involved in the mechanism of photoaging and carcinogenesis. Skin is endowed with antioxidant enzymes including superoxide dismutases (SOD): cytosolic copper zinc SOD and mitochondrial manganese SOD. The aim of our study was to estimate the protective effect of manganese against oxidative injury on cultured human skin fibroblasts. Dithranol, hydrogen peroxide and UV-A radiation (375 nm) were employed as oxidative stressors. The supply of manganese chloride produced an increase in cellular content of this element up to 24 fold without concomitant elevation of MnSOD activity. Nevertheless, manganese protects cells against two of the three ROS generating systems assessed, namely hydrogen peroxyde and UV-A. This protective effect depends on the concentration of manganese in the medium, 0.1 mM and 0.2 mM protect against UVA cytotoxicity, only 0.2 mM protects against H2O2 cytotoxicity. 相似文献
52.
Alain E. Reinberg 《Chronobiology international》1993,10(4):235-237
53.
Gruber Véronique Berna Patrick P. Arnaud Thierry Bournat Philippe Clément Christèle Mison Dominique Olagnier Béatrice Philippe Laurence Theisen Manfred Baudino Sylvie Bénicourt Claude Cudrey Claire Bloës Carole Duchateau Nathalie Dufour Sylvie Gueguen Catherine Jacquet Séverine Ollivo Catherine Poncetta Christine Zorn Nathalie Ludevid Dolores Van Dorsselaer Alain Verger Robert Doherty Annette Mérot Bertrand Danzin Charles 《Molecular breeding : new strategies in plant improvement》2001,7(4):329-340
A recombinant dog gastric lipase with therapeutic potential for the treatment of exocrine pancreatic insufficiency was expressed in transgenic tobacco plants. We targeted the protein using two different signal sequences for either vacuolar retention or secretion. In both cases, an active glycosylated recombinant protein was obtained. The recombinant enzymes and the native enzyme displayed similar properties including acid resistance and acidic optimum pH. The proteolytic maturation and the specific activity of the recombinant proteins, however, were found to be dependent on subcellular compartmentalization. Expression levels of recombinant dog gastric lipase were about 5% and 7% of acid extractable plant proteins for vacuolar retention and secretion respectively. This expression system already has allowed the production of tens of grams of purified lipase through open-field culture of transgenic tobacco plants. 相似文献
54.
55.
56.
57.
Abstract Plasmid pIP501 was transferred by conjugation from Lactococcus lactis to Lactobacillus delbrückii subsp. bulgaricus and Lactobacillus helveticus . Only Lb. delbrückii subsp. bulgaricus transconjugants could act as a donor in crosses with Lc. lactis . No Lactobacillus transconjugants were detected after inter- or intra-species Lactobacillus crosses. Plasmid pIP501 has undergone no detectable deletion or rearrangement during transfer from Lc. lactis to Lactobacillus strains. 相似文献
58.
59.
60.
E Laplantine E Fontan J Chiaravalli T Lopez G Lakisic M Véron F Agou Alain Israël 《The EMBO journal》2009,28(19):2885-2895
An important property of NEMO, the core element of the IKK complex involved in NF‐κB activation, resides in its ability to specifically recognize poly‐ubiquitin chains. A small domain called NOA/UBAN has been suggested to be responsible for this property. We recently demonstrated that the C‐terminal Zinc Finger (ZF) of NEMO is also able to bind ubiquitin. We show here by ZF swapping and mutagenesis that this represents its only function. While neither NOA nor ZF shows any preference for K63‐linked chains, we demonstrate that together they form a bipartite high‐affinity K63‐specific ubiquitin‐binding domain. A similar domain can be found in two other proteins, Optineurin and ABIN2, and can be freely exchanged with that of NEMO without interfering with its activity. This suggests that the main function of the C‐terminal half of NEMO is to specifically bind K63‐linked poly‐ubiquitin chains. We also demonstrate that the recently described binding of NEMO to linear poly‐ubiquitin chains is dependent on the NOA alone and does not require the presence of the ZF. 相似文献