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Anna Żółciak Katarzyna Sikora Justyna Anna Nowakowska Monika Małecka Małgorzata Borys Anna Tereba 《Biocontrol Science and Technology》2016,26(12):1706-1718
Polymerase chain reaction-amplified and sequenced isolates of Antrodia gossypium, Phlebiopsis gigantea and Heterobasidion parviporum from decaying Norway spruce wood blocks after three and six months, which exhibited linear growth, were investigated. P. gigantea strains showed the fastest growth, whereas A. gossypium growth was five times slower. The differences between the mean daily increment of A. gossypium and the other examined isolates (except Hp2) were statistically significant. There were also significant differences in wood decay between densities over time. These results were confirmed by the decay acceleration index (DAI) and decay activity index, which were positively correlated with wood density regardless of the fungus species. The registered P. gigantea strains (Rotstop and PG Suspension) exhibited a strong decomposition ability (28% after six months); the weight loss caused by A. gossypium after six months of decay (15.2%) was similar to the results of P. gigantea (GB) after just three months (13.2%). All tested H. parviporum isolates showed rather rapid growth and equally strong wood decay (20–25%) compared to those of P. gigantea. DAI showed that A. gossypium may significantly contribute to wood decomposition over time, particularly in less dense wood samples. The use of both saprotrophs as biological agents against root pathogens is discussed. 相似文献
33.
Michał W. Borys 《Plant and Soil》1966,24(3):476-478
Summary A breaking of petioles of potatoes is described as due to a deficient level of phosphorus in the nutrient medium. 相似文献
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Deepali Rahi Borys Dzyuba Miaomiao Xin Yu Cheng Viktoriya Dzyuba 《Journal of fish biology》2020,97(2):435-443
Sturgeon spermatozoa are unique for their sustained motility. We investigated the relative importance of bioenergetic pathways in the energy supply of Siberian sturgeon Acipenser baerii spermatozoa during motile and immotile states. Spermatozoon motility and oxygen consumption rate (OCR) were analysed following exposure to inhibitors of oxidative phosphorylation (sodium azide, NaN3), glycolysis (2-deoxy-D-glucose, DOG) and β-oxidation of fatty acids (sodium fluoride, NaF), and to an uncoupler of oxidative phosphorylation (carbonyl cyanide m-chlorophenyl hydrazine, CCCP). No significant difference in curvilinear velocity was observed after addition of these reagents to activation medium (AM) or nonactivation medium (NAM) for incubation. Incubation of spermatozoa in NAM containing CCCP or NaN3 resulted in significantly decreased motility duration compared to controls. The OCR of sturgeon spermatozoa in AM (11.9 ± 1.4 nmol O2 min−1 (109 spz)−1) was significantly higher than in NAM (8.2 ± 1.5 nmol O2 min−1 (109 spz)−1). The OCR significantly declined with addition of NaN3 to AM and NAM. No significant difference in motility parameters or OCR was observed with NaF or DOG. These results suggest active oxidative phosphorylation in both immotile and motile spermatozoa. Nevertheless, mitochondrial respiration occurring during motility is not sufficient to meet the high energy demands, and the energy required for sustained motility of Siberian sturgeon spermatozoa is derived from adenosine triphosphate accumulated during the quiescent state. 相似文献
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Aleksandra Czarna Rafael Sanjuán Fernando González-Candelas Borys Wróbel 《BMC evolutionary biology》2006,6(1):105-13
Background
The least squares (LS) method for constructing confidence sets of trees is closely related to LS tree building methods, in which the goodness of fit of the distances measured on the tree (patristic distances) to the observed distances between taxa is the criterion used for selecting the best topology. The generalized LS (GLS) method for topology testing is often frustrated by the computational difficulties in calculating the covariance matrix and its inverse, which in practice requires approximations. The weighted LS (WLS) allows for a more efficient albeit approximate calculation of the test statistic by ignoring the covariances between the distances. 相似文献39.
Wawrzkiewicz A Pawelek K Borys P Dworakowska B Grzywna ZJ 《European biophysics journal : EBJ》2012,41(6):505-526
Several approaches to ion-channel gating modelling have been proposed. Although many models describe the dwell-time distributions correctly, they are incapable of predicting and explaining the long-term correlations between the lengths of adjacent openings and closings of a channel. In this paper we propose two simple random-walk models of the gating dynamics of voltage and Ca(2+)-activated potassium channels which qualitatively reproduce the dwell-time distributions, and describe the experimentally observed long-term memory quite well. Biological interpretation of both models is presented. In particular, the origin of the correlations is associated with fluctuations of channel mass density. The long-term memory effect, as measured by Hurst R/S analysis of experimental single-channel patch-clamp recordings, is close to the behaviour predicted by our models. The flexibility of the models enables their use as templates for other types of ion channel. 相似文献
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Borys Szefczyk M. Natália D. S. Cordeiro Ricardo Franco José A. N. F. Gomes 《Journal of biological inorganic chemistry》2009,14(7):1119-1128
Molecular dynamics simulations of the wild-type and variant forms of the mouse ferrochelatase in complex with the product
(haem) have been performed using the GROMOS96 force field, in the NpT ensemble. Ferrochelatase, the last enzyme in the catalytic
pathway of the haem biosynthesis, catalyses the reaction of insertion of a ferrous ion into protoporphyrin IX by distorting
the planar geometry of the latter reactant. The simulations presented aim at understanding the role of active-site residues
in this catalytic process. Analysis of the simulation trajectories explains the consequences of the mutations introduced and
sheds more light on the role of the His209 residue in porphyrin macrocycle distortion. The function of residues coordinating
propionate groups of the haem molecule is discussed in terms of stability of the substrate and product complexes. 相似文献