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981.
Willeke MC van Roon-Mom Barry A Pepers Peter AC 't Hoen Carola ACM Verwijmeren Johan T den Dunnen Josephine C Dorsman GertJan B van Ommen 《BMC molecular biology》2008,9(1):84
Background
Huntington's disease is a progressive autosomal dominant neurodegenerative disorder that is caused by a CAG repeat expansion in the HD or Huntington's disease gene. Although micro array studies on patient and animal tissue provide valuable information, the primary effect of mutant huntingtin will inevitably be masked by secondary processes in advanced stages of the disease. Thus, cell models are instrumental to study early, direct effects of mutant huntingtin. mRNA changes were studied in an inducible PC12 model of Huntington's disease, before and after aggregates became visible, to identify groups of genes that could play a role in the early pathology of Huntington's disease. 相似文献982.
Spatial and temporal variation of phytoplankton in two subtropical Brazilian reservoirs 总被引:3,自引:2,他引:1
Paula Aparecida Federiche Borges Sueli Train Luzia Cleide Rodrigues 《Hydrobiologia》2008,607(1):63-74
The purpose of this study was to verify the longitudinal distribution of phytoplankton biomass in two subtropical Brazilian
reservoirs in the State of Paraná and investigate intervening factors on changes in phytoplankton biomass according to functional
groups. In the Capivari and Segredo reservoirs, samples were obtained every 3 months during 2002, along a longitudinal axis
(fluvial, transition, and lacustrine zones) at different depths. One hundred and eighteen taxa were identified, with Chlorophyceae
as the most specious group. During the study period, both reservoirs had mostly low biomass values (less than 1 mm3 l−1). The short retention time of these reservoirs constituted the principal limiting factor to phytoplankton development. Biomass
values above 1 mm3 l−1 were observed in the Capivari fluvial zone in March and in the Segredo lacustrine zone in December, with dominance by Microcystis aeruginosa Kütz (LM) and Anabaena circinalis Rab. (H1), respectively. Vertical and horizontal gradients of analyzed abiotic variables and phytoplankton biomass were observed.
Considering the phytoplankton biomass values, both reservoirs were oligotrophic for the duration of the study. The Canonical
Correspondence Analysis (CCA) evidenced temporal and spatial gradients of phytoplankton biomass; nevertheless, it did not
follow the classic model proposed for deep reservoirs, since higher biomass was registered in the lacustrine zone during some
months and in fluvial zones during other months. Distinct functional groups of phytoplankton characterized both studied reservoirs.
Capivari Reservoir was best characterized by LM and Y groups, indicative of its greater water column stability and higher phosphorus concentration, whereas Segredo Reservoir
was principally characterized by the MP functional group, indicative of its greater mixing zone extension and higher nitrate
concentration. The obtained results also evidenced the influence of morphometric conditions and watershed purposes as important
structuring factors of phytoplankton biomass in these reservoirs.
Handling editor: L. Naselli-Flores 相似文献
983.
de Macedo Braga LM Lacchini S Schaan BD Rodrigues B Rosa K De Angelis K Borges LF Irigoyen MC Nardi NB 《Journal of biomedical science》2008,15(3):365-374
This work aimed to evaluate cardiac morphology/function and histological changes induced by bone marrow cells (BMCs) and cultured
mesenchymal stem cells (MSCs) injected at the myocardium of spontaneously hypertensive rats (SHR) submitted to surgical coronary
occlusion. Female syngeneic adult SHR, submitted (MI) or not (C) to coronary occlusion, were treated 24 h later with in situ
injections of normal medium (NM), or with MSCs (MSC) or BMCs (BM) from male rats. The animals were evaluated after 1 and 30 days
by echocardiography, histology of heart sections and PCR for the Y chromosome. Improved ejection fraction and reduced left
ventricle infarcted area were observed in MSC rats as compared to the other experimental groups. Treated groups had significantly
reduced lesion tissue score, increased capillary density and normal (not-atrophied) myocytes, as compared to NM and C groups.
The survival rate was higher in C, NM and MSC groups as compared to MI and BM groups. In situ injection of both MSCs and BMCs
resulted in improved cardiac morphology, in a more physiological model of myocardial infarction represented by surgical coronary
occlusion of spontaneously hypertensive rats. Only treatment with MSCs, however, ameliorated left ventricle dysfunction, suggesting
a positive role of these cells in heart remodeling in infarcted hypertensive subjects. 相似文献
984.
Laboratory cages were used to evaluate the influence of extraguild (EGprey) and intraguild prey (IGprey) densities on the direction, symmetry and magnitude of the intraguild predation (IGP) of the aphidophagous Harmonia axyridis Pallas on Coccinella undecimpunctata L. and vice versa. In order to understand the role of competition between IGprey, the experiments included treatments with one IGpredator, with one or four IGprey and EGprey (Aphis fabae Scopoli) ranging from zero to sufficient aphids to satiate the predators for 12, 24 or 48 h. Increases in EGprey and IGprey densities did not alter the direction, but decreased the magnitude and symmetry of IGP. Predation on one individual of IGprey decreased from more than 80%, in the absence of EGprey, to from 6% to 53%, at higher EGprey densities. Decrease in IGP was less when H. axyridis was the IGpredator. Even at high EGprey densities, eggs and 2nd larval stages of C. undecimpunctata were vulnerable to IGP and the level of predation was 40% and 53%. The presence of more than one IGprey increased the magnitude of IGP mainly at EGprey densities sufficient to satiate the predators for 12 and 24 h, suggesting that competition between the IGpredator and IGprey may be one of the processes promoting IGP. These results and those of other authors suggest that H. axyridis has the potential to be an IGpredator, mainly of the most vulnerable stages of IGprey. Thus, H. axyridis may negatively affect the survival of C. undecimpunctata, when these two species exploit the same resources. 相似文献
985.
Borges AA Campos GM Moreli ML Moro Souza RL Saggioro FP Figueiredo GG Livonesi MC Moraes Figueiredo LT 《Microbes and infection / Institut Pasteur》2008,10(10-11):1150-1157
The hantavirus pulmonary syndrome (HPS) is an emerging syndrome in the Americas. The disease results from intense immune activation and changes in vascular permeability. The aim of this study was to determine the profile of serum cytokines in HPS patients looking for correlation with the clinical parameters, severity and outcome of illness. Studying 21 HPS patients, we found that IL-6 may have an important role in the pathogenesis of HPS, being associated with fatal outcome. Our results also support a mixed Th1/Th2 immune response during the course of HPS and that the magnitude of Th1 response effector cytokines is correlated to HPS severity. The decreased levels of TGF-beta observed in HPS patients suggest that immunoregulatory activity could be damaged in these patients. 相似文献
986.
987.
988.
989.
Parente JA Borges CL Bailão AM Felipe MS Pereira M de Almeida Soares CM 《Mycopathologia》2008,165(4-5):259-273
The ascomycete Paracoccidioides brasiliensis is a human pathogen with a broad distribution in Latin America. The infection process of P. brasiliensis is initiated by aerially dispersed mycelia propagules, which differentiate into the yeast parasitic phase in human lungs. Therefore, the transition to yeast is an initial and fundamental step in the infective process. In order to identify and characterize genes involved in P. brasiliensis transition to yeast, which could be potentially associated to early fungal adaptation to the host, expressed sequence tags (ESTs) were examined from a cDNA library, prepared from mycelia ongoing differentiation to yeast cells. In this study, it is presented a screen for a set of genes related to protein synthesis and to protein folding/modification/destination expressed during morphogenesis from mycelium to yeast. Our analysis revealed 43 genes that are induced during the early transition process, when compared to mycelia. In addition, eight novel genes related to those processes were described in the P. brasiliensis transition cDNA library. The types of induced and novel genes in the transition cDNA library highlight some metabolic aspects, such as putative increase in protein synthesis, in protein glycosylation, and in the control of protein folding that seem to be relevant to the fungal transition to the parasitic phase. 相似文献
990.
Summary A new and probably endemic species of Cambessedesia DC. is described from campo rupestre vegetation, as part of a floristic survey of Melastomataceae from the S?o José mountain range, Tiradentes District, Minas Gerais State, Brazil.
Miscellaneous Accepted for publication April 2008. 相似文献