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Protection and deprotection of horse cytochrome c 总被引:1,自引:0,他引:1
The last step in the semisynthesis of horse cytochrome c analogues (formation of the bond 65-66) requires the conformation of the complex between two complementary fragments, (1-65) lactone and (66-104). The fragments can be obtained from a limited degradation with cyanogen bromide. The amino component in this reaction can also be obtained from organo chemical synthesis in which the C-terminal fragment (81-104) is required in a selectively protected form. The latter is available from a cyanogen bromide degradation of ubiquitously protected cytochrome c. The details of the protection/deprotection reaction and the properties of nonadecamethylsulfonylethyloxycarbonyl cytochrome c are described. 相似文献
13.
Occurrence of P503 in microorganisms 总被引:1,自引:0,他引:1
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Boon Seng Ooi Beatrice T. M. Chen Charles C. S. Toh Oon Teik Khoo 《BMJ (Clinical research ed.)》1970,3(5725):744-746
Of 127 hypertensive patients aged 12 to 40 investigated by intravenous pyelography, abdominal aortography, and renal biopsy an underlying cause was found in 57%. The proportion with secondary hypertension was higher in young patients and in those with severe hypertension. Primary arteritis of the aorta was an important cause of renovascular hypertension in an Asian population. 相似文献
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Amelia K. Kivaisi Huub J. M. Op den Camp Henk J. Lubberding Jaap J. Boon Godfried D. Vogels 《Applied microbiology and biotechnology》1990,33(1):93-98
Summary The supernatants of effluents from an artificial rumen reactor degrading barley straw have been shown to contain lignin-derived compounds by UV spectral characteristics and pyrolysis mass spectrometry (PYMS). Most of these compounds were shown to be released by the action of rumen microorganisms. The compounds were quantified by measuring absorbance at 280 nm using bamboo-milled wood lignin as a standard. The concentration of the compounds rose from 0.5 mg·ml–1 at solid and liquid retention times (SRT and HRT) of 60 and 12 h, respectively, and a loading rate (LR) of 25 g total solids (TS)·l–1 per day to 3.5 mg·ml–1 at a SRT of 144 h, an HRT of 20 days and an LR of 15 g TS·1–1 per day. The highest concentration was below the level known to be toxic to rumen microorganisms in vitro. No indications were found for anaerobic lignin degradation in the rumen reactor.
Offprint requests to: H. J. M. Op den Camp 相似文献
18.
W Musters K Boon C A van der Sande H van Heerikhuizen R J Planta 《The EMBO journal》1990,9(12):3989-3996
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A new gene coding for an antigen recognized by autologous cytolytic T lymphocytes on a human renal carcinoma 总被引:7,自引:0,他引:7
Béatrice Gaugler Nathalie Brouwenstijn Valérie Vantomme Jean-Pierre Szikora Corry W. Van der Spek Jean-Jacques Patard Thierry Boon Peter Schrier Benoît J. Van den Eynde 《Immunogenetics》1996,44(5):323-330
Previous reports have described antigens that are recognized on human melanoma cells by autologous cytolytic T lymphocytes
(CTL). The genes coding for a number of these antigens have been identified. Here we report the cloning of a gene that codes
for an antigen recognized by autologous CTL on a human renal carcinoma cell line. This antigen is presented byHLA-B7 and is encoded by a new gene that we have namedRAGE1. No expression ofRAGE1 was found in normal tissues other than retina. RAGE1 expression was found in only one of 57 renal cell carcinoma samples,
and also in some sarcomas, infiltrating bladder carcinomas, and melanomas. This represents the first identification of an
antigen recognized by autologous CTL on a renal tumor. 相似文献