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71.
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Exploring Biotic and Abiotic Determinants of Nest Size in Mediterranean Great Tits (Parus major) and Blue Tits (Cyanistes caeruleus) 下载免费PDF全文
Marcel M. Lambrechts Jacques Blondel Cyril Bernard Samuel P. Caro Anne Charmantier Virginie Demeyrier Claire Doutrelant Gabrielle Dubuc‐Messier Amélie Fargevieille Christophe de Franceschi Pablo Giovannini Arnaud Grégoire Sylvie Hurtrez‐Boussès Annick Lucas Mark C. Mainwaring Pascal Marrot Adèle Mennerat Samuel Perret Philippe Perret 《Ethology : formerly Zeitschrift fur Tierpsychologie》2016,122(6):492-501
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Estimating the abundance of burrow‐nesting species through the statistical analysis of combined playback and visual surveys 下载免费PDF全文
Anne‐Sophie Bonnet‐Lebrun Licia Calabrese Gerard Rocamora Andrés López‐Sepulcre 《Journal of avian biology》2016,47(5):642-649
The conservation of elusive species relies on our ability to obtain unbiased estimates of their abundance trends. Many species live or breed in cavities, making it easy to define the search units (the cavity) yet hard to ascertain their occupancy. One such example is that of certain colonial seabirds like petrels and shearwaters, which occupy burrows to breed. In order to increase the chances of detection for these types of species, their sampling can be done using two independent methods to check for cavity occupancy: visual inspection, and acoustic response to a playback call. This double‐detection process allows us to estimate the probability of burrow occupancy by accounting for the probability of detection associated with each method. Here we provide a statistical framework to estimate the occupancy and population size of burrow‐dwelling species. We show how to implement the method using both maximum likelihood and Bayesian approaches, and test its precision and bias using simulated datasets. We subsequently illustrate how to extend the method to situations where two different species may occupy the burrows, and apply it to a dataset on wedge‐tailed shearwaters Puffinus pacificus and tropical shearwaters P. bailloni on Aride Island, Seychelles. The simulations showed that the single‐species model performed well in terms of error and bias except when detection probabilities and occupancies were very low. The two‐species model applied to shearwaters showed that detection probabilities were highly heterogeneous. The population sizes of wedge‐tailed and tropical shearwaters were estimated at 13 716 (95% CI: 12 909–15 874) and 25 550 (23 667–28 777) pairs respectively. The advantages of formulating the call‐playback sampling method statistically is that it provides a framework to calculate uncertainty in the estimates and model assumptions. This method is applicable to a variety of cavity‐dwelling species where two methods can be used to detect cavity occupancy. 相似文献
75.
Sequencing the human genome has allowed the discovery of millions of DNA sequence variants. Sequence variations in human DNA are mainly present asSingle Nucleotide Polymorphisms (SNPs); this common form of variation is found about once every 1,000 bases in the human genome and 1.8 million SNPs have now been identified and located. The accessibility of databases of SNPs opens the possibility of studying the influence of these polymorphisms on disease risks as well as on drug responses. Numerous approaches have been set up for the identification of SNPs. In this review we describe the main techniques used for the identification of these polymorphisms. They rely on two major consequences of sequence variations: the apparition or the disappearance of restriction enzyme sites or the alteration of DNA strand hybridization due to the presence of a mismatch. Southern blotting and restriction endonucleases have allowed the development of the technique ofrestriction fragment length polymorphisms (RFLPs), now performed on PCR products. Several other approaches such as denaturing high-performance liquid chromatography or real-time PCR can detect allele differences upon re-hybridization and heteroduplex formation. However, DNA sequencing remains the obligate step for the positive identification of known or unknown SNPs. At last, the development of high-throughput methods allows a large increase in the rate of discovery of SNPs likely. 相似文献
76.
Van Leene J Stals H Eeckhout D Persiau G Van De Slijke E Van Isterdael G De Clercq A Bonnet E Laukens K Remmerie N Henderickx K De Vijlder T Abdelkrim A Pharazyn A Van Onckelen H Inzé D Witters E De Jaeger G 《Molecular & cellular proteomics : MCP》2007,6(7):1226-1238
Defining protein complexes is critical to virtually all aspects of cell biology because many cellular processes are regulated by stable protein complexes, and their identification often provides insights into their function. We describe the development and application of a high throughput tandem affinity purification/mass spectrometry platform for cell suspension cultures to analyze cell cycle-related protein complexes in Arabidopsis thaliana. Elucidation of this protein-protein interaction network is essential to fully understand the functional differences between the highly redundant cyclin-dependent kinase/cyclin modules, which are generally accepted to play a central role in cell cycle control, in all eukaryotes. Cell suspension cultures were chosen because they provide an unlimited supply of protein extracts of actively dividing and undifferentiated cells, which is crucial for a systematic study of the cell cycle interactome in the absence of plant development. Here we report the mapping of a protein interaction network around six known core cell cycle proteins by an integrated approach comprising generic Gateway-based vectors with high cloning flexibility, the fast generation of transgenic suspension cultures, tandem affinity purification adapted for plant cells, matrix-assisted laser desorption ionization tandem mass spectrometry, data analysis, and functional assays. We identified 28 new molecular associations and confirmed 14 previously described interactions. This systemic approach provides new insights into the basic cell cycle control mechanisms and is generally applicable to other pathways in plants. 相似文献
77.
78.
M. Christiane Brahimi-Horn Sandra Lacas-Gervais Ricardo Adaixo Karine Ilc Matthieu Rouleau Annick Notte Marc Dieu Carine Michiels Thibault Voeltzel Vronique Maguer-Satta Joffrey Pelletier Marius Ilie Paul Hofman Bndicte Manoury Alexander Schmidt Sebastian Hiller Jacques Pouyssgur Nathalie M. Mazure 《Molecular and cellular biology》2022,42(1)
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S Guibaud M Bonnet C Coicaud G Sournies A Combet J M Thoulon M Dumont 《Journal de génétique humaine》1980,28(1):61-62
From an observation, authors underline the interest of two complementary tests: ultrasonography and amniotic fluid AFP determination. About the reported case, the chronology of facts is unusual: ultrasonography led initially to suspect anomaly, then elevated AFP result confirmed the malformation. 相似文献