The biochemical aspects of pineal gland functioning in the rat in ontogeny

We have studied metabolic pathways of serotonin in epiphysis of Wistar male rats at the age of 1; 1,5; 2; 3; 6; 12; 24 and 36 months. Epiphysis retains its functional activity throughout the life, however, serotonin metabolism in epiphysis undergoes marked changes. The main regulatory principle is the switch of serotonin metabolic pathways in the pineal gland. Most distinct changes between hydroxy- and methoxyindols in epiphysis have been observed during the periods of age-associated hormonal rearrangements like sexual maturation or involution of the gonad function at the old age.     

Signal molecules during the organism development: Central and peripheral sources of noradrenaline in rat ontogenesis

Using the method of high performance liquid chromatography with electrochemical detection, the age dynamics of the content of noradrenaline (NA) in the brain, adrenal gland, and the organ of Zuckerkandl in prenatal (18th and 21st days of embryogenesis) and early postnatal (3, 7, 15, and 30th days) periods of development was studied. The potential contribution of these organs to the formation of physiologically active concentration of noradrenalin in the blood was also assessed. The results suggest that, during the development of the organism, the activity of the sources of noradrenaline in the general circulation changes, which gives a reason to assume the existence of humoral interaction between NA-producing organs in the perinatal period of ontogenesis.     

Use of therapeutic polycomponent vaccine "Immunovac VP-4" for the treatment of chronic bacterial prostatitis

A complex of clinical and laboratory examinations of patients with bacterial chronic prostatitis was carried out. The patients were divided into two groups: 55 patients treated by the method of immunotherapy and 20 patients (controls) undergoing basic therapy. Immunotherapy was carried out with the use of polycomponent vaccine VP-4 containing Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris and Escherichia coli antigens. Vaccinal therapy was indicated in cases with infection focus associated with opportunistic bacteria, a prolonged torpid course of prostatitis and when different schemes of etiotropic therapy proved to be ineffective. The vaccine was introduced in 4 or 5 subcutaneous injections at an interval of 5-7 days. The study revealed that in cases of chronic prostatitis accompanied by microecological and immune disturbances the use of complex therapy allowed to achieve good clinical and laboratory results in 91% of the patients. In the patients undergoing immunotherapy the elimination of opportunistic bacteria from the urogenital tract and the restoration of affected microflora occurred.     

The β-amylase polymorphism of winter common wheat grains

The polymorphism of winter common wheat with respect to β-amylase isoenzymes has been analyzed using electrophoresis in polyacrylamide gel (PAAG) buffered with a Tris-glycine system (pH 8.3). Seven β-amylase isoenzymes have been found in wheat varieties and the breeding stocks. Isoenzymes A, B, and C are the most frequent in Russian and Ukrainian varieties (51.7 ± 4.7, 30.7 ± 3.8, and 11.9 ± 2.5%, respectively). Two alleles of the β-Amy-D1 locus of the long arm of chromosome 4D have been identified. The substrate-enzyme affine effect can be used to locate the zones of activity of this enzyme by means of staining for proteins. It has been determined that β-amylase isoenzymes may play a role in the aggregating capacity of the grain protein complex via the formation of S-S bonds.     

Formation of electron transport barriers under ECR control of the q(r) profile in the T-10 tokamak

Abstract-the formation of transport barriers under electron cyclotron resonance heating and current drive in the t-10 tokamak is studied. in regimes with off-axis co-eccd and q _L <4 at the limiter, a spontaneous transition to improved confinement accompanied by the formation of two electron transport barriers is observed. the improvement resembles an L-H transition. It manifests itself as density growth, a decrease in the D_α emission intensity, and an increase in the central electron and ion temperatures. Two deep wells on the potential profile (the first one at r/a _L ≈0.6, where a _L is the limiter radius, and the second one near the edge) arise during the transition. the internal barrier is formed when dq/dr～0 with q≈1 in the barrier region.     

The adhesion of clinical isolates of Campylobacter jejuni to intestinal epithelial cells in vitro

The adhesive activity of C. jejuni isolated from feces of children with Campylobacter infection was studied with the use of a newly developed model. 47 clinical isolates were analyzed; of these, 91% were found to be enteroadhesive to a variable degree. As the result of in vitro studies, Campylobacter were found to have much greater tropism to colonic cells and epithelial cells of Peyer's patches in comparison with the epithelial cells of the small intestine. The correlation between the degree of adhesive activity and the severity of the course of Campylobacter infection in children.     

Role of the instrument transmission factor in the epidemiology of viral hepatitis. II. Methods, materials, chief results]

The authors conducted a prospective controlled epidemiological observation in two towns of the Moldavian SSR. There were 50 000 persons under observation. Over 300 000 parenteral manipulations carried out in them were recorded. Analysis of the materials obtained on the Minsk-22 computer demonstrated the same incidence of viral hepatitis in the groups given parenteral manipulations and without them. Consequently, the instrumental transmission factor had no essential significance in the epidemiology of viral hepatitis, this corresponding to the results of the authors' preceding theoretical study.     

Experimental study, using a pulmonary model, of the pathogenic properties of genetically related strains of Sh. flexneri, differing in their ability to cause keratoconjunctivitis]

Complex microbiological and morphological study of the genetically connected Sh. flexneri strains, avirulent by keratoconjunctival test showed that the greatest extent of the virulent loss was revealed in shigellae with mutation in the area glpK-gene: they lost the capacity to penetrate into the cells of the bronchial epithelium, to resist local leukocytic reaction and to produce an injurious action on the pulmonary vessels. Shigellae with the replaced kcpA- gene area mostly lost the capacity to penetrate into the epithelial cells of mouse bronchi and were eliminated from the epithelium by the 9th hour after the administration. Xyl--avir-hybrids obtained from crossing of Sh flexneri with the streptomycin-resistant E. coli K12 donors were characterized by the loss of the capacity to prolonged reproduction in the organism of mice with the retention of the capacity to penetration into the epithelium, of the influence on the development of the leukocytic reaction and of the damaging effect on the pulmonary capillaries.