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51.
I. J. Bom J. P. P. M. Smelt K. Kersters C. T. Verrips 《Journal of applied microbiology》1986,60(6):483-490
Strains of Clostridium botulinum type A, type E and both non-proteolytic and proteolytic types B and F were characterized by their electrophoretic protein patterns. As the protein pattern changes during sporulation, special attention was paid to the prevention of sporulation by selecting an appropriate medium (Strasdine's medium plus 1% w/v glucose) and a scheme of repeated subculturing. Ribosomal proteins, evolutionarily conservative and hence relatively similar in all types of bacteria, were removed to optimize the resolving power of the electrophoretic technique. Protein patterns were compared by computing correlation coefficients of normalized densitometric tracings. The method is highly reproducible and its resolving power is high: all protein patterns found were specific. The strains tested fall into two main groups: the proteolytic and the non-proteolytic cluster. Type A strains form a separate subgroup within the proteolytic cluster, the same applies to type E strains within the non-proteolytic group. Although time-consuming for spore-forming bacteria, this method is, to our knowledge, the only technique that recognizes individual strains of Cl. botulinum . For non-spore-forming micro-organisms the method is certainly much simpler and hence even more valuable. 相似文献
52.
All 17 examined Polish isolates of Fusarium moniliforme Sheldon [syn. F. verticillioides (Sacc.) Nirenberg], originating from wheat, maize and barley kernels, exhibited pathogenicity to wheat seedlings of the cultivar ‘Almari’ (5 isolates-very strong, 3 strong, 3 medium, 5 weak and only one very weak). The same isolates were able to produce the mycotoxins fumonisins on rice under laboratory conditions. Fumonisin B1 (FB1) was detected in concentrations ranging from 1.3 to 2240mg/kg and fumonisin B2(FB2) from 0.7 to 600 mg/kg. The yield of the fumonisins produced in vitro was not related to the pathogenicity of individual isolates. 相似文献
53.
D R Barn A Bom J Cottney W L Caulfield J R Morphy 《Bioorganic & medicinal chemistry letters》1999,9(9):1329-1334
Two focused libraries of delta opioid ligands were synthesised using AlCl3 facilitated aminolysis. Several compounds were identified with DOR binding affinities higher or similar to SNC-80. A novel acyclic derivative of SNC-80 produced antinociception in the acetic acid abdominal constriction test, which is at least partially mediated via the delta-opioid receptor. 相似文献
54.
Mutations in the WFS1 gene that cause low-frequency sensorineural hearing loss are small non-inactivating mutations 总被引:3,自引:0,他引:3
Cryns K Pfister M Pennings RJ Bom SJ Flothmann K Caethoven G Kremer H Schatteman I Köln KA Tóth T Kupka S Blin N Nürnberg P Thiele H van de Heyning PH Reardon W Stephens D Cremers CW Smith RJ Van Camp G 《Human genetics》2002,110(5):389-394
Hereditary hearing impairment is an extremely heterogeneous trait, with more than 70 identified loci. Only two of these loci are associated with an auditory phenotype that predominantly affects the low frequencies (DFNA1 and DFNA6/14). In this study, we have completed mutation screening of the WFS1 gene in eight autosomal dominant families and twelve sporadic cases in which affected persons have low-frequency sensorineural hearing impairment (LFSNHI). Mutations in this gene are known to be responsible for Wolfram syndrome or DIDMOAD (diabetes insipidus, diabetes mellitus, optic atrophy, and deafness), which is an autosomal recessive trait. We have identified seven missense mutations and a single amino acid deletion affecting conserved amino acids in six families and one sporadic case, indicating that mutations in WFS1 are a major cause of inherited but not sporadic low-frequency hearing impairment. Among the ten WFS1 mutations reported in LFSNHI, none is expected to lead to premature protein truncation, and nine cluster in the C-terminal protein domain. In contrast, 64% of the Wolfram syndrome mutations are inactivating. Our results indicate that only non-inactivating mutations in WFS1 are responsible for non-syndromic low-frequency hearing impairment. 相似文献
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56.
Bom Roeland A. van Gils Jan A. Molenaar Karen Kwarteng Andy Y. Victor Reginald Folmer Eelke O. 《Hydrobiologia》2021,848(3):751-752
Hydrobiologia - In the above mentioned publication, incorrect values for P. segnis are shown on the right hand side of Fig. 4. The correct version of Fig. 4 and its caption is... 相似文献
57.
Andr Tavares da Silva Fernandes Silvia Bahadian Moreira Luciane Pinto Gaspar Marisol Simes Ana Carolina dos Reis Albuquerque Cajaraville Renata Carvalho Pereira Mariana Pierre de Barros Gomes Jos Henrique Rezende Linhares Vanessa de Oliveira Santos Renata Tourinho Santos Juliana Fernandes Amorim Tamiris Azamor da Costa Barros Juliana Gil Melgao Andra Marques Vieira da Silva Camilla Bayma Fernandes Luciana Neves Tubaro Jane da Silva Elena Cristina Caride Maria Beatriz Borges Rosane Cuber Guimares Renato Srgio Marchevsky Sheila Maria Barbosa de Lima Ana Paula Dinis Ano Bom Patrícia Cristina da Costa Neves Alcides Pissinatti Marcos da Silva Freire 《Journal of medical primatology》2021,50(1):36-45
58.
Suppression of Water Absorption by Molecular Design of Ionic Liquid Electrolyte for Li–Air Battery
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Morgan L. Thomas Yoshiki Oda Ryoichi Tatara Hoi‐Min Kwon Kazuhide Ueno Kaoru Dokko Masayoshi Watanabe 《Liver Transplantation》2017,7(3)
In this study, the effect of water on the oxygen reduction reaction is initially investigated in aprotic ionic liquids by cyclic voltammetry, revealing that the presence of water significantly deteriorates the reversibility of the oxygen reduction reaction and oxygen evolution reaction, which will be detrimental to performance of a practical lithium–air battery. In order to prevent moisture intrusion from ambient air, a hydrophobic electrolyte is then designed and the moisture content in the electrolyte exposed to ambient air is greatly suppressed, to less than 1 wt% at 30 °C and 90% RH (relative humidity). Charge and discharge tests using the hydrophobic electrolyte are reversible for several cycles even in O2 with a RH of 28%. This approach for enhancing the hydrophobicity of the electrolyte, and thus the inherent equilibrium moisture content, for the Li–air battery demonstrates the possibility for improved stability to ambient moisture. 相似文献
59.
Levy CB Stumbo AC Ano Bom AP Portari EA Cordeiro Y Carneiro Y Silva JL De Moura-Gallo CV 《The international journal of biochemistry & cell biology》2011,43(1):60-64
P53 is one of the most important tumor suppressor proteins in human cancers. Mutations in the TP53 gene are common features of malignant tumors and normally correlate to a more aggressive disease. In breast cancer, these gene alterations are present in approximately 20% of cases and are characteristically of missense type. In the present work we describe TP53 mutations in breast cancer biopsies and investigate whether wild and mutant p53 participate in protein aggregates formation in these breast cancer cases. We analyzed 88 biopsies from patients residing in the metropolitan area of Rio de Janeiro, and performed TP53 mutation screening using direct sequencing of exons 5-10. Seventeen mutations were detected, 12 of them were of missense type, 2 nonsenses, 2 deletions and 1 insertion. The presence of TP53 mutation was highly statistically associated to tumor aggressiveness of IDC cases, indicated here by Elston Grade III (p<0.0001). Paraffin embedded breast cancer tissues were analyzed for the presence of p53 aggregates through immunofluorescence co-localization assay, using anti-aggregate primary antibody A11, and anti-p53. Our results show that mutant p53 co-localizes with amyloid-like protein aggregates, depending on mutation type, suggesting that mutant p53 may form aggregates in breast cancer cells, in vivo. 相似文献
60.
Hubert Josien David Bom Dennis P. Curran Yu-Huang Zheng Ting-Chao Chou 《Bioorganic & medicinal chemistry letters》1997,7(24):1574-3194
The synthesis and biological evaluation of about one dozen 7-silylcamptothecin derivatives are described. Most new compounds show potencies comparable to or better than camptothecin itself. The best compound, 11-fluoro-10-amino-7-trimethylsilylcamptothecin, is more than 20 times more potent than camptothecin in cell assays. 相似文献