全文获取类型
收费全文 | 5352篇 |
免费 | 435篇 |
国内免费 | 1篇 |
出版年
2023年 | 23篇 |
2022年 | 59篇 |
2021年 | 138篇 |
2020年 | 79篇 |
2019年 | 98篇 |
2018年 | 145篇 |
2017年 | 108篇 |
2016年 | 194篇 |
2015年 | 268篇 |
2014年 | 280篇 |
2013年 | 391篇 |
2012年 | 443篇 |
2011年 | 484篇 |
2010年 | 294篇 |
2009年 | 264篇 |
2008年 | 306篇 |
2007年 | 354篇 |
2006年 | 299篇 |
2005年 | 261篇 |
2004年 | 264篇 |
2003年 | 248篇 |
2002年 | 197篇 |
2001年 | 49篇 |
2000年 | 42篇 |
1999年 | 45篇 |
1998年 | 54篇 |
1997年 | 41篇 |
1996年 | 43篇 |
1995年 | 32篇 |
1994年 | 23篇 |
1993年 | 19篇 |
1992年 | 26篇 |
1991年 | 21篇 |
1990年 | 22篇 |
1989年 | 16篇 |
1988年 | 14篇 |
1987年 | 9篇 |
1986年 | 7篇 |
1985年 | 9篇 |
1984年 | 9篇 |
1983年 | 10篇 |
1982年 | 9篇 |
1981年 | 10篇 |
1980年 | 10篇 |
1979年 | 5篇 |
1978年 | 8篇 |
1976年 | 6篇 |
1973年 | 5篇 |
1971年 | 6篇 |
1935年 | 4篇 |
排序方式: 共有5788条查询结果,搜索用时 171 毫秒
991.
Julian Maggini Gerardo Mirkin Ianina Bognanni Josefina Holmberg Isabel M. Piazzón Irene Nepomnaschy Héctor Costa Cristian Ca?ones Silvina Raiden Mónica Vermeulen Jorge R. Geffner 《PloS one》2010,5(2)
In recent years it has become clear that the therapeutic properties of bone marrow-derived mesenchymal stromal cells (MSC) are related not only to their ability to differentiate into different lineages but also to their capacity to suppress the immune response. We here studied the influence of MSC on macrophage function. Using mouse thioglycolate-elicited peritoneal macrophages (M) stimulated with LPS, we found that MSC markedly suppressed the production of the inflammatory cytokines TNF-α, IL-6, IL-12p70 and interferon-γ while increased the production of IL-10 and IL-12p40. Similar results were observed using supernatants from MSC suggesting that factor(s) constitutively released by MSC are involved. Supporting a role for PGE2 we observed that acetylsalicylic acid impaired the ability of MSC to inhibit the production of inflammatory cytokines and to stimulate the production of IL-10 by LPS-stimulated M. Moreover, we found that MSC constitutively produce PGE2 at levels able to inhibit the production of TNF-α and IL-6 by activated M. MSC also inhibited the up-regulation of CD86 and MHC class II in LPS-stimulated M impairing their ability to activate antigen-specific T CD4+ cells. On the other hand, they stimulated the uptake of apoptotic thymocytes by M. Of note, MSC turned M into cells highly susceptible to infection with the parasite Trypanosoma cruzi increasing more than 5-fold the rate of M infection. Using a model of inflammation triggered by s.c. implantation of glass cylinders, we found that MSC stimulated the recruitment of macrophages which showed a low expression of CD86 and the MHC class II molecule Iab and a high ability to produce IL-10 and IL-12p40, but not IL-12 p70. In summary, our results suggest that MSC switch M into a regulatory profile characterized by a low ability to produce inflammatory cytokines, a high ability to phagocyte apoptotic cells, and a marked increase in their susceptibility to infection by intracellular pathogens. 相似文献
992.
Urban metabolism accounts of total annual energy, water, and other resource flows are increasingly available for a variety of world cities. For local decision makers, however, it may be important to understand the variations of resource consumption within the city. Given the difficulty of gathering suburban resource consumption data for many cities, this article investigates the potential of statistical downscaling methods to estimate local resource consumption using socioeconomic or other data sources. We evaluate six classes of downscaling methods: ratio‐based normalization; linear regression (both internally and externally calibrated); linear regression with spatial autocorrelation; multilevel linear regression; and a basic Bayesian analysis. The methods were applied to domestic energy consumption in London, UK, and our results show that it is possible to downscale aggregate resource consumption to smaller geographies with an average absolute prediction error of around 20%; however, performance varies widely by method, geography size, and fuel type. We also show how mapping these results can quickly identify districts with noteworthy resource consumption profiles. Further work should explore the design of local data collection strategies to enhance these methods and apply the techniques to other urban resources such as water or waste. 相似文献
993.
History rather than hybridization determines population structure and adaptation in Populus balsamifera
下载免费PDF全文
![点击此处可从《Journal of evolutionary biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
P. G. Meirmans J. Godbout M. Lamothe S. L. Thompson N. Isabel 《Journal of evolutionary biology》2017,30(11):2044-2058
Hybridization between species is known to greatly affect their genetic diversity and, therefore, their evolution. Also, within species, there may be genetic clusters between which gene flow is limited, which may impact natural selection. However, few studies have looked simultaneously at the influence of among‐species and within‐species gene flow. Here, we study the influence of hybridization between Populus balsamifera and Populus trichocarpa on population structure and adaptation in P. balsamifera. We did this by sampling a total of 1517 individuals from across the ranges of these two species, and by genotyping them using a combination of 93 nuclear and 17 cpDNA SNPs. We found that hybridization is mostly limited to the contact zone where the species’ distributions overlap. Within P. balsamifera, we found multiple levels of population structure. Interestingly, the border between the Eastern and Central clusters is very sharp, whereas the border between the Central and Western clusters is diffuse. Outlier analysis revealed that three loci associated with the sharp border were also associated with climate. We hypothesize that the observed clusters derive from three refugia during the Pleistocene ice ages. Between the Central and Western clusters, post‐glacial long‐distance gene flow has led to the diffusion of their border. In the Eastern cluster, we hypothesize that endogenous genomic barriers have developed, leading to the sharp border and a spurious climate association. We conclude that the large‐scale genetic structure of P. balsamifera is mostly shaped by historical factors and the influence of interspecific hybridization is limited. 相似文献
994.
J. Julio Camarero Juan Carlos Linares Ana I. García-Cervigón Enric Batllori Isabel Martínez Emilia Gutiérrez 《Ecosystems》2017,20(4):683-700
Alpine treeline ecotones are considered early-warning monitors of the effects of climate change on terrestrial ecosystems, but it is still unclear how accurately treeline dynamics may track the expected temperature rises. Site-specific abiotic constraints, such as topography and demographic trends may make treelines less responsive to environmental fluctuations. A better understanding on how local processes modulate treelines’ response to warming is thus required. We developed a model of treeline dynamics based on individual data of growth, mortality and reproduction. Specifically, we modeled growth patterns, mortality rates and reproductive size thresholds as a function of temperature and stand structure to evaluate the influence of climate- and stand-related processes on treeline dynamics. In this study, we analyze the dynamics of four Pyrenean mountain pine treeline sites with contrasting stand structures, and subjected to differing rates of climate warming. Our models indicate that Pyrenean treelines could reach basal areas and reproductive potentials similar to those currently observed in high-elevation subalpine forest by the mid twenty-first century. The fastest paces of treeline densification are forecasted by the late twenty-first century and are associated with higher warming rates. We found a common densification response of Pyrenean treelines to climate warming, but contrasting paces arise due to current size structures. Treelines characterized by a multistratified stand structure and subjected to lower mean annual temperatures were the most responsive to climate warming. In monostratified stands, tree growth was less sensitive to temperature than in multistratified stands and trees reached their reproductive size threshold later. Therefore, our simulations highlight that stand structure is paramount in modulating treeline responsiveness to ongoing climate warming. Synthesis. Treeline densification over the twenty-first century is likely to occur at different rates contingent on current stand structure and its effects on individual-level tree growth responses to warming. Accurate projections of future treeline dynamics must thus incorporate site-specific factors other than climate, specifically those related to stand structure and its influence on tree growth. 相似文献
995.
The Pseudomonas putida CsrA/RsmA homologues negatively affect c‐di‐GMP pools and biofilm formation through the GGDEF/EAL response regulator CfcR
下载免费PDF全文
![点击此处可从《Environmental microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
996.
Jatropha curcas and assisted phytoremediation of a mine tailing with biochar and a mycorrhizal fungus 总被引:1,自引:0,他引:1
Ma. del Carmen A. González-Chávez Rogelio Carrillo-González María Isabel Hernández Godínez Silvia Evangelista Lozano 《International journal of phytoremediation》2017,19(2):174-182
Soil pollution is an important ecological problem worldwide. Phytoremediation is an environmental-friendly option for reducing metal pollution. A greenhouse experiment was conducted to determine the growth and physiological response, metal uptake, and the phytostabilization potential of a nontoxic Jatropha curcas L. genotype when grown in multimetal-polluted conditions. Plants were established on a mine residue (MR) amended or not amended with corn biochar (B) and inoculated or not inoculated with the mycorrhizal fungus Acaulospora sp. (arbuscular mycorrhizal fungus, AMF). J. curcas was highly capable of growing in an MR and showed no phytotoxic symptoms. After J. curcas growth (105 days), B produced high desorption of Cd and Pb from the MR; however, no increases in metal shoot concentrations were observed. Therefore, Jatropha may be useful for phytostabilization of metals in mine tailings. The use of B is recommended because improved MR chemical properties conduced to plant growth (cation-exchange capacity, organic matter content, essential nutrients, electrical conductivity, water-holding capacity) and plant growth development (higher biomass, nutritional and physiological performance). Inoculation with an AMF did not improve any plant growth or physiological plant characteristic. Only higher Zn shoot concentration was observed, but it was not phytotoxic. Future studies of B use and its long-term effect on MR remediation should be conducted under field conditions. 相似文献
997.
Lourdes Farre Gabriela Sanz Neus Ruiz-Xivill Manuel Castro de Moura Juan Francisco Martin-Tejera Samuel Gonalves-Ribeiro Maria Martinez-Iniesta Monica Calaf Jose Luis Mosquera Jos Ignacio Martín-Subero Isabel Granada Manel Esteller Eva Domingo-Domenech Fina Climent Alberto Villanueva Anna Sureda 《Disease models & mechanisms》2021,14(7)
998.
999.
Sofia Karabatsos Nicholas C. Larter Danny G. Allaire Kayla Eykelboom Cesar Estevo Majid Iravani Isabel C. Barrio David S. Hik 《The Journal of wildlife management》2024,88(3):e22536
Across most of their native North American range, the horns of mountain sheep (Ovis spp.) males are getting smaller, a pattern attributed to selective hunting pressure. We measured the horns of 755 Dall's sheep males (Ovis dalli dalli) in the southern Mackenzie Mountains, Northwest Territories, between 2002 and 2017. For each male, we measured the circumference and length of each annulus for the right horn and calculated horn volume for each year. We examined changes in horn size in 4 different outfitter areas, using age at harvest as a covariate. Hunting pressure across years in the study area was consistently low, and this population did not experience the decline in horn size observed in several other mountain sheep populations in Canada. Over the 16-year period, the average horn volume of harvested males was stable and even increased in 1 outfitter area. Local management of Dall's sheep delivered independently by the guide outfitters in the Mackenzie Mountains appears to contribute to maintaining a population of males that has not been adversely affected by strong selective hunting pressure. The resilience of this management strategy may be challenged by environmental changes associated with rapid warming in northern mountain environments. 相似文献
1000.
Conventional complex media are routinely used to grow auxotrophic strains under the assumption that they can compensate the latter's nutritional deficiencies. We here demonstrate that this is not always true. This study compares the growth parameters of Saccharomyces cerevisiae (S288C) and its derived auxotrophic strains FY1679-14C and BY4741 in synthetic minimal medium (SD), standard YPD medium from two of the most commonly used suppliers, or modified YPD medium. Maximum specific growth rates of auxotrophic strains were slightly lower than the prototrophic case in all growth conditions tested. Also, the biomass production of auxotrophic strains in synthetic medium was slightly less than the prototrophic case. However in both of the two standard YPD media used, the biomass production of both auxotrophic strains was markedly lower than that of the prototrophic one. The extent of the differences depended on the medium used. Indeed in one of the two YPD media, the lower biomass production of auxotrophic strains was evident even at the diauxic shift. Uracil seems to be the main limiting growth factor for both auxotrophic strains growing in the two standard YPD medium tested. No YPD media or specific supplement was able to compensate for the effect of the auxotrophic mutations in the multiple auxotrophic marker strain BY4741. The fact that auxotrophic strains grew poorly on YPD when compared to their prototrophic counterpart indicates that standard YPD medium is not sufficient to overcome the effect of auxotrophic mutations. 相似文献