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排序方式: 共有109条查询结果,搜索用时 375 毫秒
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62.
Hormonally regulated programmed cell death in barley aleurone cells 总被引:13,自引:0,他引:13
Cell death was studied in barley (cv Himalaya) aleurone cells treated with abscisic acid and gibberellin. Aleurone protoplasts incubated in abscisic acid remained viable in culture for at least 3 weeks, but exposure to gibberellin initiated a series of events that resulted in death. Between 4 and 8 days after incubation in gibberellin, >70% of all protoplasts died. Death, which occurred after cells became highly vacuolated, was manifest by an abrupt loss of plasma membrane integrity followed by rapid shrinkage of the cell corpse. Hydrolysis of DNA began before death and occurred as protoplasts ceased production of alpha-amylase. DNA degradation did not result in the accumulation of discrete low molecular weight fragments. DNA degradation and cell death were prevented by LY83583, an inhibitor of gibberellin signaling in barley aleurone. We conclude that cell death in aleurone cells is hormonally regulated and is the final step of a developmental program that promotes successful seedling establishment. 相似文献
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HYUK JE LEE ELIZABETH G. BOULDING 《Biological journal of the Linnean Society. Linnean Society of London》2010,100(3):494-505
Natural populations of widely‐distributed animals often exhibit clinal variation in phenotypic traits or in allele frequencies of a particular gene over their geographical range. A planktotrophic intertidal snail, Littorina keenae is broadly distributed along the north‐eastern Pacific coast through a large latitudinal range (24°50′N–43°18′N). We tested for latitudinal clines in two complex phenotypic traits – thermal tolerance and body size – and one single locus trait – heat shock cognate 70 (HSC70) – in L. keenae along almost its entire geographical range. We found only weak evidence for a latitudinal cline in the thermal tolerance and no evidence for a cline in allele frequencies at HSC70. However, as predicted by Bergmann's rule, we detected a strong latitudinal cline that accounted for 60% of the variance in body size (R2 = 0.598; P < 0.001). In contrast, body size did not significantly affect thermal tolerance. HSC70 showed no genetic differentiation among the populations, supporting our previous mitochondrial gene‐based estimate of high gene flow during this snail's free‐swimming larval stage. Given that L. keenae experiences panmixia along its species range, the observed size cline may be partially or entirely caused by a phenotypically plastic response to local thermal environments rather than by genetic divergence in body size among populations in response to locally optimizing natural selection. © 2010 The Linnean Society of London, Biological Journal of the Linnean Society, 2010, 100 , 494–505. 相似文献
66.
Megalodiscus temperatus (Stafford, 1905) is a common paramphistome trematode of North American amphibians with a two host life cycle and has been reported to infect frogs and rarely tadpoles. In this study we document the alternative life cycle strategy of M. temperatus in tadpoles and metamorphosed anurans. We show through field work and experimental infections that M. temperatus can establish in both anuran life stages and worms become gravid and release eggs in both tadpoles and metamorphosed frogs. However, worms exhibit differences in route of infection, development, egg production, and diet in tadpoles and metamorphosed anurans. These alternative life history strategies of M. temperatus suggest different selective pressures on the development and reproductive success of these worms in tadpoles and metamorphosed anurans, and we discuss the evolutionary avenues for and constraints on amphibian trematode life cycles presented by these two different anuran life stages. 相似文献
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Michaël C Fontaine Stuart JE Baird Sylvain Piry Nicolas Ray Krystal A Tolley Sarah Duke Alexei Birkun Jr Marisa Ferreira Thierry Jauniaux Ángela Llavona Bayram Öztürk Ayaka A Öztürk Vincent Ridoux Emer Rogan Marina Sequeira Ursula Siebert Gísli A Vikingsson Jean-Marie Bouquegneau Johan R Michaux 《BMC biology》2007,5(1):1-16
Background
Understanding the role of seascape in shaping genetic and demographic population structure is highly challenging for marine pelagic species such as cetaceans for which there is generally little evidence of what could effectively restrict their dispersal. In the present work, we applied a combination of recent individual-based landscape genetic approaches to investigate the population genetic structure of a highly mobile extensive range cetacean, the harbour porpoise in the eastern North Atlantic, with regards to oceanographic characteristics that could constrain its dispersal.Results
Analyses of 10 microsatellite loci for 752 individuals revealed that most of the sampled range in the eastern North Atlantic behaves as a 'continuous' population that widely extends over thousands of kilometres with significant isolation by distance (IBD). However, strong barriers to gene flow were detected in the south-eastern part of the range. These barriers coincided with profound changes in environmental characteristics and isolated, on a relatively small scale, porpoises from Iberian waters and on a larger scale porpoises from the Black Sea.Conclusion
The presence of these barriers to gene flow that coincide with profound changes in oceanographic features, together with the spatial variation in IBD strength, provide for the first time strong evidence that physical processes have a major impact on the demographic and genetic structure of a cetacean. This genetic pattern further suggests habitat-related fragmentation of the porpoise range that is likely to intensify with predicted surface ocean warming. 相似文献68.
MT Butcher JW Hermanson NG Ducharme LM Mitchell LV Soderholm JE Bertram 《Comparative biochemistry and physiology. Part A, Molecular & integrative physiology》2009,152(1):100-114
The forelimb digital flexors of the horse display remarkable diversity in muscle architecture despite each muscle-tendon unit having a similar mechanical advantage across the fetlock joint. We focus on two distinct muscles of the digital flexor system: short compartment deep digital flexor (DDF(sc)) and the superficial digital flexor (SDF). The objectives were to investigate force-length behavior and work performance of these two muscles in vivo during locomotion, and to determine how muscle architecture contributes to in vivo function in this system. We directly recorded muscle force (via tendon strain gauges) and muscle fascicle length (via sonomicrometry crystals) as horses walked (1.7 m s(-1)), trotted (4.1 m s(-1)) and cantered (7.0 m s(-1)) on a motorized treadmill. Over the range of gaits and speeds, DDF(sc) fascicles shortened while producing relatively low force, generating modest positive net work. In contrast, SDF fascicles initially shortened, then lengthened while producing high force, resulting in substantial negative net work. These findings suggest the long fibered, unipennate DDF(sc) supplements mechanical work during running, whereas the short fibered, multipennate SDF is specialized for economical high force and enhanced elastic energy storage. Apparent in vivo functions match well with the distinct architectural features of each muscle. 相似文献
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Larvae of certain species of blowflies (Calliphoridae) cause myiasis in amphibians which may result in significant mortality, yet there are few reports from North America. In this study, we observed primary myiasis in a population of juvenile eastern American toads (Bufo americanus americanus) collected during May-July 1998 from southeastern Wisconsin (USA). Nine (6%) of 140 toads were infected by the green blow fly (Bufolucilia silvarum) with a mean intensity of 10.5 +/- 7.2 (range = 1-24). Weekly parasite prevalence and mean intensity remained low, ranging from 0-20% and 2 +/- 1.4 to 14 +/- 6, respectively. We found: 1) flies lay eggs on healthy toads, 2) eggs hatch with first instar maggots penetrating under the skin, 3) maggots develop to mature third instars within 5-7 days, 4) maggots leave the host and form pupa within 8-11 days of hatching, and 5) maggots pupate within 7-9 days at room temperature. All infected toads died within 1-2 wk as a result of the infection. The low prevalence observed in this study and other reports of this species from mammalian and bird carcasses indicated that B. silvarum is probably a facultative parasite of toads and other amphibians in the United States. This is the first report of B. silvarum causing myiasis in Wisconsin amphibians and the first report in eastern American toads in the United States. 相似文献
70.
A total of 584 adult nematomorphs, Gordius difficilis, was collected from 2 man-made ponds and their overflow stream in southeastern Wisconsin. Ponds were surveyed throughout the year, but all free-living worms were found during July-August of 1996, July-September of 1997, and June-August of 1998. Overall sex ratio was male biased; however, sex ratio was variable during different months. Observations during 1998-2000 indicated that worms mated within 24-48 hr of emergence from their hosts and began laying eggs by mid-August, continuing until mid-October. Eggs with well-developed larvae were recovered during October and November. Encysted larval nematomorphs were recovered from aquatic and semiaquatic invertebrates (gastropods, earthworms, and insects), whereas developing hairworms were found in terrestrial European ground beetles Pterostichus melanarius. It is hypothesized that semiaquatic invertebrates may serve as intermediate/paratenic hosts in this system and are preyed upon by terrestrial carabid beetles, thus completing the life cycle. In addition, scanning electron microscopy observations of G. difficilis add previously unreported observations on intraspecific variation in body length, cuticle morphology, and gametes of this species. This is the first report of G. difficilis from Wisconsin as well as the first report of this species from P. melanarius and aquatic and semiaquatic invertebrates. 相似文献