全文获取类型
收费全文 | 524篇 |
免费 | 40篇 |
国内免费 | 3篇 |
专业分类
567篇 |
出版年
2021年 | 6篇 |
2018年 | 4篇 |
2017年 | 7篇 |
2016年 | 9篇 |
2015年 | 23篇 |
2014年 | 21篇 |
2013年 | 30篇 |
2012年 | 27篇 |
2011年 | 27篇 |
2010年 | 19篇 |
2009年 | 14篇 |
2008年 | 20篇 |
2007年 | 20篇 |
2006年 | 12篇 |
2005年 | 22篇 |
2004年 | 17篇 |
2003年 | 21篇 |
2002年 | 11篇 |
2001年 | 24篇 |
2000年 | 12篇 |
1999年 | 14篇 |
1998年 | 10篇 |
1997年 | 18篇 |
1996年 | 4篇 |
1995年 | 4篇 |
1994年 | 6篇 |
1993年 | 7篇 |
1992年 | 6篇 |
1991年 | 10篇 |
1990年 | 4篇 |
1989年 | 4篇 |
1988年 | 10篇 |
1987年 | 5篇 |
1986年 | 5篇 |
1985年 | 7篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 4篇 |
1980年 | 8篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 7篇 |
1975年 | 8篇 |
1974年 | 15篇 |
1973年 | 5篇 |
1972年 | 9篇 |
1971年 | 7篇 |
1966年 | 3篇 |
1961年 | 2篇 |
排序方式: 共有567条查询结果,搜索用时 0 毫秒
81.
D R Barres M A Duhr Y A Boivin 《Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology》1985,7(4):320-326
Morphologically typical uterine cervical biopsies were separated into normal cervices, condylomas and cervical intraepithelial neoplasias (CIN) grades I, II and III. At least 100 nuclei per lesion were measured on 4 micron Feulgen-stained sections using a Zeiss microspectrophotometer, with a variant of the plug method used to compute the nuclear DNA content. DNA distribution histograms were then decomposed into subsets of diploid, tetraploid, octoploid and aneuploid cells. The decomposition, which assumed a log-normal model of polydiploidy distribution, led to the identification of six indices: (1) the percentage of diploid cells, (2) the percentage of tetraploid cells, (3) the percentage of octoploid cells, (4) the percentage of aneuploid cells with DNA contents less than tetraploidy, (5) the percentage of aneuploid cells with DNA contents between tetraploidy and octaploidy and (6) the percentage of aneuploid cells with DNA contents greater than octoploidy. These indices, along with the mean nuclear radius, the 5c exceeding rate and the 2c deviation index, generated a nine-dimensional space. Two methods of discriminant analysis on this space showed discriminating powers of 78.22% and 87.13%, respectively, as compared to the original diagnoses. The most discriminating variable in both analyses appeared to be the percentage of octoploid cells. 相似文献
82.
Phosphofructokinase (PFK) isozymes of blood cells and some human tissues were studied by starch gel electrophoresis and immunoprecipitation by anti-muscle and anti-erythrocyte PFK sera. PFK from muscle, heart, brain and placenta were totally precipitated by both antisera. PFK from blood cells (erythrocytes, lymphocytes, granulocytes, platelets) were precipitated more strongly by anti-erythrocyte PFK serum than by anti-muscle PFK serum. Liver, kidney and monoblast PFK were slightly precipitated by both antisera. From the electrophoretic patterns and the immunoprecipitation curves we may conclude that muscle contains the homotetrameric M4 forms; platelet, liver and kidney the homotetrameric E4 form, and blood cells the M-E hybrids. Monoblasts probably contain a E4 type PFK precursor, and heart, placenta and brain, a modified M4 type PFK. Other isozymes, unrelated with muscle and erythrocyte, were revealed in liver and kidney. 相似文献
83.
84.
85.
Summary The erythrocytic and liver pyruvate kinases (PK) from a patient with congenital nonspherocytic hemolytic anemia have been studied. In red blood cells, the residual activity, 28% of the normal control, presented normal kinetic properties, instability to heat and urea, and slow electrophoretic mobility. The L-type PK from the patient's liver was characterized by normal activity, kinetic properties, stability to heat and urea, and electrophoretic mobility. The fact that erythrocyte mutant PK may, as in previous reports, or may not be associated, as in the present observation, with molecular abnormalities of the liver PK provides support for the hypothesis of a gene rearrangement compatible with two different tissue-specific mRNAs. 相似文献
86.
Nina Bertaux-Skeirik Rui Feng Michael A. Schumacher Jing Li Maxime M. Mahe Amy C. Engevik Jose E. Javier Richard M. Peek Jr Karen Ottemann Veronique Orian-Rousseau Gregory P. Boivin Michael A. Helmrath Yana Zavros 《PLoS pathogens》2015,11(2)
The cytotoxin-associated gene (Cag) pathogenicity island is a strain-specific
constituent of Helicobacter pylori (H. pylori) that
augments cancer risk. CagA translocates into the cytoplasm where it stimulates cell
signaling through the interaction with tyrosine kinase c-Met receptor, leading
cellular proliferation. Identified as a potential gastric stem cell marker,
cluster-of-differentiation (CD) CD44 also acts as a co-receptor for c-Met, but
whether it plays a functional role in H. pylori-induced epithelial
proliferation is unknown. We tested the hypothesis that CD44 plays a functional role
in H. pylori-induced epithelial cell proliferation. To assay changes
in gastric epithelial cell proliferation in relation to the direct interaction with
H. pylori, human- and mouse-derived gastric organoids were
infected with the G27 H. pylori strain or a mutant G27 strain
bearing cagA deletion (∆CagA::cat). Epithelial proliferation
was quantified by EdU immunostaining. Phosphorylation of c-Met was analyzed by
immunoprecipitation followed by Western blot analysis for expression of CD44 and
CagA. H. pylori infection of both mouse- and human-derived gastric
organoids induced epithelial proliferation that correlated with c-Met
phosphorylation. CagA and CD44 co-immunoprecipitated with phosphorylated c-Met. The
formation of this complex did not occur in organoids infected with
∆CagA::cat. Epithelial proliferation in response to
H. pylori infection was lost in infected organoids derived from
CD44-deficient mouse stomachs. Human-derived fundic gastric organoids exhibited an
induction in proliferation when infected with H. pylorithat was not
seen in organoids pre-treated with a peptide inhibitor specific to CD44. In the
well-established Mongolian gerbil model of gastric cancer, animals treated with CD44
peptide inhibitor Pep1, resulted in the inhibition of H.
pylori-induced proliferation and associated atrophic gastritis. The current
study reports a unique approach to study H. pylori interaction with
the human gastric epithelium. Here, we show that CD44 plays a functional role in
H. pylori-induced epithelial cell proliferation. 相似文献
87.
88.
Can Wide Consultation Help with Setting Priorities for Large-Scale Biodiversity Monitoring Programs?
Climate and other global change phenomena affecting biodiversity require monitoring to track ecosystem changes and guide policy and management actions. Designing a biodiversity monitoring program is a difficult task that requires making decisions that often lack consensus due to budgetary constrains. As monitoring programs require long-term investment, they also require strong and continuing support from all interested parties. As such, stakeholder consultation is key to identify priorities and make sound design decisions that have as much support as possible. Here, we present the results of a consultation conducted to serve as an aid for designing a large-scale biodiversity monitoring program for the province of Québec (Canada). The consultation took the form of a survey with 13 discrete choices involving tradeoffs in respect to design priorities and 10 demographic questions (e.g., age, profession). The survey was sent to thousands of individuals having expected interests and knowledge about biodiversity and was completed by 621 participants. Overall, consensuses were few and it appeared difficult to create a design fulfilling the priorities of the majority. Most participants wanted 1) a monitoring design covering the entire territory and focusing on natural habitats; 2) a focus on species related to ecosystem services, on threatened and on invasive species. The only demographic characteristic that was related to the type of prioritization was the declared level of knowledge in biodiversity (null to high), but even then the influence was quite small. 相似文献
89.
90.
Catherine Boivin Ibrahima Ndoye Flore Molouba Philippe de Lajudie Nicolas Dupuy Bernard Dreyfus 《植物科学评论》1997,16(1):1-30
Rhizobia can establish a nitrogen-fixing symbiosis with plants of the Leguminosae family. They elicit on their host plant the formation of new organs, called nodules, which develop on the roots. A few aquatic legumes, however, can form nodules on their stem at dormant root primordia. The stem-nodulating legumes described so far are all members of the genera Aeschynomene, Sesbania, Neptunia, and Discolobium. Their rhizobial symbionts belong to four genera already described: Rhizobium, Bradyrhizobium, Sinorhizobium, and Azorhizobium. This review summarizes our current knowledge on most aspects of stem nodulation in legumes, the infection process and nodule development, the characterization and unusual features of the associated bacteria, and the molecular genetics of nodulation. Potential use as green manure in lowland rice of these stem-nodulating legumes, giving them agronomical importance, is also discussed. 相似文献