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An anchored restriction-mapping approach applied to the genetic analysis of the Anopheles gambiae malaria vector complex 1 总被引:1,自引:0,他引:1
We introduce here a simple approach for rapidly determining restriction
maps for a number of regions of a genome; this involves "anchoring" a map
with a rare restriction site (in this case the seldom-cutting EagI)
followed by partial digestion of a frequent-cutting enzyme (e.g., Sau 3A).
We applied this technology to five species of the Anopheles gambiae
complex. In a single Southern blot we obtained about a 15-kb restriction
map each for the mtDNA, rRNA gene, and a scnDNA region for each of five
species. Phylogenetic analyses of these regions yield trees at odds with
the more traditional chromosome inversion-based trees. The value of the
approach for systematic purposes is the ease with which several large,
independent regions of the genome can be quickly assayed for molecular
variation.
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Testing lack of fit in multiple regression 总被引:2,自引:0,他引:2
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Establishment and characterization of somatic hybrids between human differentiated macrophages and mouse myeloma NS1 cells 总被引:1,自引:0,他引:1
A Bohbot A Faradji M Schmitt F Oberling F Uttwiller J V Ruch S Braun D Hartmann J Bartholeyns P Poindron 《International journal of cell cloning》1990,8(1):63-79
Human macrophages obtained from circulating monocytes matured in vitro by culture for seven days in hydrophobic flexible teflon bags were successfully fused with murine myeloma NS1 cells. Six of 20 clones, selected for their adherence properties, were further studied. All possessed human chromosomes (mean number ranging from 4 to 14 depending on the clones studied), exhibited non-specific esterases (but no peroxidase activity) and expressed CD14 antigen and C3 receptors (but no MAX-1 antigen). Moreover, the hybridomas retained phagocytic activity and high interferon plus lipopolysaccharide-activable cytolytic activity against tumor cells. 相似文献
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Alain Bohbot Francoise Uttwiller Ricardo Fujita Benedicte Mousson Michel Fabre Francis Oberling Philippe Poindron 《In vitro cellular & developmental biology. Animal》1993,29(5):362-370
Summary Despite more than 50 attempts and the use of various methods, it has been impossible to establish homologous hybridomas between
human mature macrophages and 8-azaguanine-resistant U-937 clones prepared in the laboratory. To rule out the possibility that
these clones were unsuitable for the selection of hybrids, a study of their properties was done. It was shown that U-937 wild
type cells were able to produce HPRT, whereas 8-azaguanine (8-aza)-resistant clones did not. Curiously, exonic and intronic
HPRT sequences were undetectable both in wild type and in 8-aza-resistant cell genomes, under conditions where they were detected
in control cells. Chromosome analysis of the clone UM9, one of the most frequently used in fusion experiments, revealed many
qualitative and quantitative differences with the U-937 wild type cells. 8-aza-resistant U-937 cells were capable of fusion
with human macrophages and gave rise to heterokaryons and probably to synkaryons, which survived for weeks without dividing
in hypoxanthine-aminopterin-thymidine medium. The results could be interpreted in terms of the existence of a transacting
negative regulatory mechanism of the macrophage genome on the proliferative capacity of homospecific hybridomas. 相似文献
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