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81.
82.
Expression of the cell-binding domain of human fibronectin in E. coli. Identification of sequences promoting full to minimal adhesive function 总被引:2,自引:0,他引:2
M Obara M S Kang S Rocher-Dufour A Kornblihtt J P Thiery K M Yamada 《FEBS letters》1987,213(2):261-264
Two cDNA subfragments containing the cell-attachment site of human fibronectin (FN) were expressed as beta-galactosidase fusion proteins in E. coli. The products were purified to homogeneity by monoclonal antibody affinity chromatography and assayed for activity in a standard cell-adhesion assay. A fusion protein containing an 80 kDa fragment of human FN appeared functionally equivalent to intact FN purified from human plasma, whereas a truncated fusion protein of 33 kDa still containing a previously postulated cell-attachment site was approx. 50-fold less active. Our study establishes a system for analyzing adhesive protein function by DNA manipulation, rules out any major role for eukaryotic post-translational modifications in FN adhesive function, and localizes additional functional activity to a 1.3 kb region. 相似文献
83.
In rabbit pulmonary artery, dopamine (10(-11)-10(-5) M) produced a concentration-dependent relaxation of the arterial strips contracted with prostaglandin F2 alpha (PGF2 alpha) in the presence of prazosin (10(-6) M), yohimbine (10(-6) M), propranolol (10(-6) M), and methysergide (10(-6) M). SKF38393, an agonist for D1 or DA1 dopamine receptor, mimicked partially the concentration-response curve for dopamine, whereas LY171555 and apomorphine did not. The order of potency of dopamine antagonists on the inhibitory effect was: cis-flupenthixol greater than bulbo-capnine greater than metoclopramide greater than haloperidol. Sulpiride was inactive. Cis-flupenthixol did not block the relaxation induced by acetylcholine, adenosine, and papaverine. In the arterial strips of the rabbits pretreated with 6-hydroxydopamine, the concentration-response curve for dopamine was similar to that in non-treated rabbits. Thus it is concluded that a specific dopamine receptor is located on the postsynaptic muscle membrane of the rabbit pulmonary artery. 相似文献
84.
Leszczynski Przemyslaw Hendrich Andrzej Boguslaw Szmida Elzbieta Sasiadek Maria Malgorzata 《BMB reports》2013,46(3):139-150
The ADAM and ADAMTS families, also called adamalysins belong to an important group of extracellular matrix proteins. The ADAMs family belong to both the transmembrane and secreted proteins, while ADAMTS family only contains secreted forms. Adamalysins play an important role in the cell phenotype regulation via their activities in signaling pathways, cell adhesion and migration. The human proteome contains 21 ADAM, and 19 ADAMTS proteins, which are involved in extracellular matrix remodeling, shedding of various substrates such as: adhesion ligands, growth factors, their receptors and diverse cytokines. Recent studies provide evidence that adamalysins play a crucial role in colorectal cancer (CRC) etiopathogenesis. It seems possible that adamalysins might be used as CRC prediction markers or potential pharmaceutical targets. [BMB Reports 2013; 46(3): 139-150] 相似文献
85.
Yoshiro Suzuki David Chitayat Hirotake Sawada Matthew A. Deardorff Heather M. McLaughlin Amber Begtrup Kathryn Millar Jennifer Harrington Karen Chong Maian Roifman Katheryn Grand Makoto Tominaga Fumio Takada Shirley Shuster Megumi Obara Hiroshi Mutoh Reiko Kushima Gen Nishimura 《American journal of human genetics》2018,102(6):1104-1114
86.
Viorica Railean-Plugaru Pawel Pomastowski Kinga Meller Michal Złoch Katarzyna Rafinska Boguslaw Buszewski 《Applied microbiology and biotechnology》2018,102(10):4423-4423
Applied Microbiology and Biotechnology - The authors would like to inform readers of a mistake in the acknowledgement section of the original publication of this article. 相似文献
87.
88.
A game model is developed of the daily schedule of matesearching activity by male butterflies, assuming that each male maximizes his expected mating success given a limited total time for mate search. The model predicts that (1) in the early morning, no male is active even though many females are emerging; (2) at a critical time, many males suddenly become active; and (3) the male's maximum activity occurs after the peak female emergence and before the peak capture efficiency. The inverse problem is also analyzed, in which the temporal pattern of capture efficiency is estimated from the knowledge of male activity and female emergence, assuming the evolutionarily stable strategy (ESS) condition. The model is then applied to data from a cabbage white butterfly (Pieris rapae crucivora) population and predicts that (1) females remain unmated for several hours on average after emergence, and (2) the male 's capture efficiency is rather low and increases significantly with time during the morning. 相似文献
89.
Acceleratory Synapses on Pacemaker Neurons in the Heart Ganglion of a Stomatopod, Squilla oratoria
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The pacemaker neurons of the heart ganglion are innervated from the CNS through two pairs of acceleratory nerves. The effect of acceleratory nerve stimulation was examined with intracellular electrodes from the pacemaker cells. The major effects on the pacemaker potential were an increase in the rate of rise of the spontaneous depolarization and in the duration of the plateau. The aftereffect of stimulation could last for minutes. No clear excitatory postsynaptic potential (EPSP) was observed, however. On high frequency stimulation, a small depolarizing response (the initial response) was sometimes observed, but the major postsynaptic event was the following slow depolarization, or the enhancement of the pacemaker potential (the late response). With hyperpolarization the initial response did not significantly change its amplitude, but the late response disappeared, showing that the latter has the property of the local response. The membrane conductance did not increase with acceleratory stimulation. The injection of depolarizing current increased the rate of rise of the spontaneous depolarization, but only slightly in comparison with acceleratory stimulation, and did not increase the burst duration. It is concluded that the acceleratory effect is not mediated by the EPSP but is due to a direct action of the transmitter on the pacemaker membrane. 相似文献
90.
In Vitro Hemolytic Characteristic of Vibrio parahaemolyticus: Its Close Correlation with Human Pathogenicity 总被引:66,自引:7,他引:59
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Yasushi Miyamoto Teiji Kato Yasushi Obara Shoichi Akiyama Kinjiro Takizawa Shiro Yamai 《Journal of bacteriology》1969,100(2):1147-1149
An in vitro hemolytic characteristic of Vibrio parahaemolyticus is closely correlated with human pathogenicity. 相似文献