The action of antifeins with different memory effects on the cAMP-independent protein kinases of brain chromatin]

The two cAMP-independent protein kinases (PK I and PK II) were obtained from neuronal chromatin of rat brain. Nonhistone HMG-proteins were used as phosphorylation substrates. The possibility of antifeines direct action on PK II was revealed. Ethylnorantifeine and its demethylated analogues increased enzyme activity whereas allylnorantifeine decreased it. The action of ethylnorantifeine and its structural analogues were similar to its influence on neuronal RNA-synthesizing activity and long-term memory. The conclusion was made that cAMP-independent PK II may be the target of antifeines action during realization of its mnestic effects.