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排序方式: 共有374条查询结果,搜索用时 15 毫秒
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Ilse M. Boudewijn Alen Faiz Katrina Steiling Erica van der Wiel Eef D. Telenga Susan J. M. Hoonhorst Nick H. T. ten Hacken Corry-Anke Brandsma Huib A. M. Kerstjens Wim Timens Irene H. Heijink Marnix R. Jonker Harold G. de Bruin J. Sebastiaan Vroegop Henk R. Pasma Wim G. Boersma Pascal Wielders Frank van den Elshout Khaled Mansour Avrum Spira Marc E. Lenburg Victor Guryev Dirkje S. Postma Maarten van den Berge 《Respiratory research》2017,18(1):213
Background
Nasal gene expression profiling is a promising method to characterize COPD non-invasively. We aimed to identify a nasal gene expression profile to distinguish COPD patients from healthy controls. We investigated whether this COPD-associated gene expression profile in nasal epithelium is comparable with the profile observed in bronchial epithelium.Methods
Genome wide gene expression analysis was performed on nasal epithelial brushes of 31 severe COPD patients and 22 controls, all current smokers, using Affymetrix Human Gene 1.0 ST Arrays. We repeated the gene expression analysis on bronchial epithelial brushes in 2 independent cohorts of mild-to-moderate COPD patients and controls.Results
In nasal epithelium, 135 genes were significantly differentially expressed between severe COPD patients and controls, 21 being up- and 114 downregulated in COPD (false discovery rate?<?0.01). Gene Set Enrichment Analysis (GSEA) showed significant concordant enrichment of COPD-associated nasal and bronchial gene expression in both independent cohorts (FDRGSEA <?0.001).Conclusion
We identified a nasal gene expression profile that differentiates severe COPD patients from controls. Of interest, part of the nasal gene expression changes in COPD mimics differentially expressed genes in the bronchus. These findings indicate that nasal gene expression profiling is potentially useful as a non-invasive biomarker in COPD.Trial registration
ClinicalTrials.gov registration number NCT01351792 (registration date May 10, 2011), ClinicalTrials.gov registration number NCT00848406 (registration date February 19, 2009), ClinicalTrials.gov registration number NCT00807469 (registration date December 11, 2008).66.
Diana O Rios‐Szwed Hironori Suzuki Andreas Kniss Frank Löhr Soichi Wakatsuki Volker Dötsch Ivan Dikic Renwick CJ Dobson David G McEwan 《EMBO reports》2017,18(8):1382-1396
Through the canonical LC3 interaction motif (LIR), [W/F/Y]‐X1‐X2‐[I/L/V], protein complexes are recruited to autophagosomes to perform their functions as either autophagy adaptors or receptors. How these adaptors/receptors selectively interact with either LC3 or GABARAP families remains unclear. Herein, we determine the range of selectivity of 30 known core LIR motifs towards individual LC3s and GABARAPs. From these, we define a I nteraction 相似文献
67.
Predation is a major ecological and evolutionary driver of natural populations, greatly influencing fitness and behaviour
of prey species. Small, long-lived petrels are vulnerable to predation at the breeding colonies and are expected to evolve
behavioural strategies to minimize predation risks. Using an automatic nest monitoring system and nightly aerial counts, we
examined the effect of vegetation cover and moonlight on colony attendance patterns and levels of burrow activity of breeding
thin-billed prions, Pachyptila belcheri, on New Island, Falkland Islands. We further investigated how these parameters were related to predation by Falkland skuas.
We monitored up to 32 nests in two habitats, one with Tussock grass and one with low vegetation cover. Individuals in both
areas were more active at the nest before hatching, and those breeding in the low cover habitat were more active and arrived
at the colony earlier, which might reflect an effect of reaction time over predation risk. Nocturnal activity peaks shifted
in time as the season progressed, indicating behavioural adjustments to sunrise hours. Moon phase did not affect attendance
and activity levels of breeders in either habitat or overall aerial activity, but influenced arrival time at the colony during
chick-rearing, individuals arriving later in periods of full moon. Skua capture rates were positively correlated with aerial
and nest activity but not with overall breeder attendance and were unaffected by moon phase. Thin-billed prions activity budgets
are influenced by environmental parameters that affect their likelihood of being predated. 相似文献
68.
Koenen ME van der Hulst R Leering M Jeurissen SH Boersma WJ 《FEMS immunology and medical microbiology》2004,40(2):119-127
Oral administration of immunoprobiotic bacteria may support animal health. Species specificity of such microorganisms requires appropriate selection. An in vitro assay for the selection of immunoprobiotic lactic acid bacteria was developed in chicken. The assay allowed testing of large numbers of individual strains. Immune stimulation in vitro correlated well with the in vivo situation in two experiments and no false negative results occurred. Therefore this assay is an appropriate selection tool for immunomodulating properties of lactic acid bacteria in chicken. 相似文献
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Jason D. Gillman Ashley Tetlow Katherine Hagely Jeffery G. Boersma Andrea Cardinal Istvan Rajcan Kristin Bilyeu 《Molecular breeding : new strategies in plant improvement》2014,34(2):447-455
The fatty acid composition of vegetable oil is becoming increasingly critical for its ultimate functionality and utilization in foods and industrial products. Partial chemical hydrogenation of soybean [Glycine max (L.) Merr.] oil increases oxidative stability and shelf life but also results in the introduction of trans fats as an unavoidable byproduct. Due to mandatory labeling of consumer products containing trans fats, conventional soybean oil has lost the ability to deliver the most appropriate economical functionality and oxidative stability, particularly for baking applications. Genetic improvement of the fatty acid profile of soybean oil is one method of meeting these new requirements for oil feedstocks. In this report, we characterized three mutant genetic loci controlling the saturated fatty acid content of soybean oil: two genes additively reduce palmitic acid content (fap1 and fap3-ug), and one gene independently elevates stearic acid content (fas). We identified a new null allele of fap3-ug/GmFATB1A (derived from line ELLP2) present in line RG3. The splicing defect mutation in a beta-ketoacyl-[acyl-carrier-protein] synthase III candidate gene located in the region mapped to fap1, derived originally from ethyl methane sulphonate mutant line C1726 (Cardinal et al. in Theor Appl Genet 127:97–111, 2014), was also present in line RG3. We also utilized the elevated stearic acid line RG7, which has previously been shown to contain novel mutant fas/SACPD-C alleles encoding stearoyl-acyl carrier protein desaturase (Boersma et al. in Crop Sci 52:1736–1742, 2012). Molecular marker assays have been developed to track these causative mutations and understand their contributions to seed oil fatty acid profiles in a recombinant inbred line population segregating for fap1, fap3-ug, and fas alleles. 相似文献
70.
CJ Cooksey 《Biotechnic & histochemistry》2014,89(5):384-387
The history, origin, identity, chemistry and uses of Congo red are described. Originally patented in 1884, Congo red soon found applications in dyeing cotton, as a pH indicator for chemists and as a biological stain. Unlike the majority of the 19th century synthetic dyes, it still is available commercially. 相似文献