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31.
Sophie Y. Dillen Véronique Storme Nicolas Marron Catherine Bastien Sabrina Neyrinck Marijke Steenackers Reinhart Ceulemans Wout Boerjan 《Tree Genetics & Genomes》2009,5(1):147-164
Interspecific hybrids of Populus species are known for their superior growth. In this study, we examined the effect of the genetic background and contrasting
environmental conditions on growth and searched for quantitative trait loci (QTL) affecting growth traits. To this end, two
hybrid poplar families resulting from controlled crosses, Populus deltoides ‘S9-2’ × P. nigra ‘Ghoy’ (D × N, 180 F1) and P. deltoides ‘S9-2’ × P. trichocarpa ‘V24’ (D × T, 182 F1), were grown at two contrasting sites, Northern Italy and Central France. At the end of the second growing season, tree dimensions
(stem height, circumference, and volume) were assessed. The performances of both families significantly differed within and
between sites. Tree volume was significantly larger at the Italian site as compared to the French site. Genotype by environment
interactions were significant but low for both families and for all growth traits. Tight correlations among the individual
growth traits indicated that there may be a common genetic mechanism with pleiotropic effects on these growth traits. In line
with previous studies, linkage groups I, VII, IX, X, XVI, XVII, and XIX appeared to have genomic regions with the largest
effects on growth traits. This study revealed that (1) both families have high potential for selection of superior poplar
hybrids due to the pronounced heterosis (hybrid vigor) and the large genetic variability in terms of growth and (2) the choice
of site is crucial for poplar cultivation.
Dillen and Storme contributed equally to the work.
An erratum to this article can be found at 相似文献
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34.
Barbara De Meester Ruben Vanholme Lisanne de Vries Marlies Wouters Jan Van Doorsselaere Wout Boerjan 《The Plant journal : for cell and molecular biology》2021,108(3):752-765
Lignin is one of the main factors determining recalcitrance to processing of lignocellulosic biomass towards bio-based materials and fuels. Consequently, wood of plants engineered for low lignin content is typically more amenable to processing. However, lignin-modified plants often exhibit collapsed vessels and associated growth defects. Vessel-specific reintroduction of lignin biosynthesis in dwarfed low-lignin cinnamoyl-CoA reductase1 (ccr1) Arabidopsis mutants using the ProSNBE:AtCCR1 construct overcame the yield penalty while maintaining high saccharification yields, and showed that monolignols can be transported between the different xylem cells acting as ‘good neighbors’ in Arabidopsis. Here, we translated this research into the bio-energy crop poplar. By expressing ProSNBE:AtCCR1 into CRISPR/Cas9-generated ccr2 poplars, we aimed for vessel-specific lignin biosynthesis to: (i) achieve growth restoration while maintaining high saccharification yields; and (ii) study the existence of ‘good neighbors’ in poplar wood. Analyzing the resulting ccr2 ProSNBE:AtCCR1 poplars showed that vessels and rays act as good neighbors for lignification in poplar. If sufficient monolignols are produced by these cells, monolignols migrate over multiple cell layers, resulting in a restoration of the lignin amount to wild-type levels. If the supply of monolignols is limited, the monolignols are incorporated into the cell walls of the vessels and rays producing them and their adjoining cells resulting in fiber hypolignification. One such fiber-hypolignified line had 18% less lignin and, despite its small yield penalty, had an increase of up to 71% in sugar release on a plant base upon saccharification. 相似文献
35.
Alexandra Bottcher Igor Cesarino Adriana Brombini dos Santos Renato Vicentini Juliana Lischka Sampaio Mayer Ruben Vanholme Kris Morreel Geert Goeminne Jullyana Cristina Magalh?es Silva Moura Paula Macedo Nobile Sandra Maria Carmello-Guerreiro Ivan Antonio dos Anjos Silvana Creste Wout Boerjan Marcos Guimar?es de Andrade Landell Paulo Mazzafera 《Plant physiology》2013,163(4):1539-1557
36.
A novel seed protein gene from Vicia faba is developmentally regulated in transgenic tobacco and Arabidopsis plants 总被引:4,自引:0,他引:4
Helmut B?Umlein Wout Boerjan Istvan Nagy Ronald Bassfüner Marc Montagu Dirk Inzé Ulrich Wobus 《Molecular & general genetics : MGG》1991,225(3):459-467
Summary We have isolated a novel gene, denoted USP, from Vicia faba var. minor, which corresponds to the most abundant mRNA present in cotyledons during early seed development; however, the corresponding protein does not accumulate in cotyledons. The characterized USP gene with its two introns is 1 of about 15 members of a gene family. A fragment comprising 637 bp of 5 flanking sequence and the total 5 untranslated region was shown to be sufficient to drive the mainly seed-specific expression of two reporter genes, coding for neomycin phosphotransferase 11 and -glucuronidase, in transgenic Arabidopsis thaliana and Nicotiana tabacum plants. We showed that the USP promoter becomes active in transgenic tobacco seeds in both the embryo and the endosperm, whereas its activity in Arabidopsis is detectable only in the embryo. Moreover, we demonstrated a transient activity pattern of the USP promoter in root tips of both transgenic host species. 相似文献
37.
Genome-wide characterization of the lignification toolbox in Arabidopsis 总被引:19,自引:0,他引:19
38.
PtABI3 impinges on the growth and differentiation of embryonic leaves during bud set in poplar 总被引:1,自引:0,他引:1 下载免费PDF全文
The Arabidopsis ABSCISIC ACID-INSENSITIVE3 (ABI3) protein plays a crucial role during late seed development and has an additional function at the vegetative meristem, particularly during periods of growth-arresting conditions and quiescence. Here, we show that the ABI3 homolog of poplar (PtABI3) is expressed in buds during natural bud set. Expression occurs clearly after perception of the critical daylength that initiates bud set and dormancy in poplar. In short-day conditions mimicking natural bud set, the expression of a chimeric PtABI3::beta-glucuronidase (GUS) gene occurred in those organs and cells of the apex that grow actively but will undergo arrest: the young embryonic leaves, the subapical meristem, and the procambial strands. If PtABI3 is overexpressed or downregulated, bud development in short-day conditions is altered. Constitutive overexpression of PtABI3 resulted in apical buds with large embryonic leaves and small stipules, whereas in antisense lines, bud scales were large and leaves were small. Thus, PtABI3 influences the size and ratio of embryonic leaves and bud scales/stipules that differentiate from the primordia under short-day conditions. These observations, together with the expression of PtABI3::GUS in embryonic leaves but not in bud scales/stipules, support the idea that wild-type PtABI3 is required for the relative growth rate and differentiation of embryonic leaves inside the bud. These experiments reveal that ABI3 plays a role in the cellular differentiation of vegetative tissues, in addition to its function in seeds. 相似文献
39.
40.
Ann Van Soom Marleen Boerjan Marie-Thrse Ysebaert Aart de Kruif 《Molecular reproduction and development》1996,45(2):171-182
Data from other laboratories have shown that speed of bovine blastocyst development is higher when Ménézo B2 is used for coculture compared to TCM199. It was our purpose to investigate whether this early blastocyst formation was also indicative of embryo quality by studying the allocation of inner cells in embryos generated by B2-coculture and by TCM199-coculture. For this purpose, a differential staining technique was used. General embryo development was similar for TCM199- and B2-embryos expressed as rate of cleavage at day 3 and morula-blastocyst formation at day 8 (P > 0.05), but significantly different when expressed as number of eight-cell stages at day 3 and expanded or hatched blastocysts at day 8 (P < 0.01). B2-embryos cultured until day 5, 6, and 7 post insemination, had total cell numbers of 24, 65, and 109 respectively, which was significantly higher than the cell number of TCM199 embryos cultured over the same time period (18, 41, and 71 respectively, P < 0.001). Morphological differentiation was significantly more advanced for B2-embryos at day 7 and 8 (P < 0.0001 and P < 0.001, respectively). First presumptive inner cells appeared in eight- to 16-cell stages at day 3. Because the determination of inner cells by differential staining is depending upon the presence of functional tight junctions, we concluded that the establishment of the tight junction seal in B2-embryos differed from that in TCM199-embryos: Inner cells appeared 0.56 cell cycle later in B2-embryos (P < 0.001) and a larger variation existed in the number of ICM-cells in B2-blastocysts (P < 0.001). The higher total cell number of B2-expanded blastocysts was mainly acquired by trophectoderm growth (P < 0.06). These data indicate that the apparent better quality of B2-embryos (faster cleavage, earlier blastocyst formation) is not reflected in a reliable number of inner cells of B2-blastocysts. © 1996 Wiley-Liss, Inc. 相似文献