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排序方式: 共有137条查询结果,搜索用时 46 毫秒
101.
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103.
Grebennikov D. S. Donets D. O. Orlova O. G. Argilaguet J. Meyerhans A. Bocharov G. A. 《Molecular Biology》2019,53(5):718-731
Molecular Biology - The modern era of research in immunology is characterized by an unprecedented level of detail about structural characteristics of the immune system and the regulation of... 相似文献
104.
Vladimir Z. Paschenko Vladimir V. Gorokhov Boris N. Korvatovskiy Eugeniy A. Bocharov Peter P. Knox Oleg M. Sarkisov Christoph Theiss Hans J. Eichler Gernot Renger Andrew B. Rubin 《BBA》2012,1817(8):1399-1406
Transient absorption changes induced by excitation of isolated reaction centers (RCs) from Rhodobacter sphaeroides with 600 nm laser pulses of 20 fs (full width at half maximum) were monitored in the wavelength region of 420–560 nm. The spectral features of the spectrum obtained are characteristic for an electrochromic band shift of the single carotenoid (Car) molecule spheroidene, which is an integral constituent of these RCs. This effect is assigned to an electrochromic bandshift of Car due to the local electric field of the dipole moment formed by electronic excitation of bacteriochlorophyll (BChl) molecule(s) in the neighborhood of Car. Based on the known distances between the pigments, the monomeric BChl (BB) in the inactive B-branch is inferred to dominate this effect. The excitation of BB at 600 nm leads to a transition into the S2 state (Qx band), which is followed by rapid internal conversion to the S1 state (Qy band), thus leading to a change of strength and orientation of the dipole moment, i.e., of the electric field acting on the Car molecule. Therefore, the time course of the electrochromic bandshift reflects the rate of the internal conversion from S2 to S1 of BB. The evaluation of the kinetics leads to a value of 30 fs for this relaxation process. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial. 相似文献
105.
Eduard V. Bocharov Pavel E. Volynsky Konstantin V. Pavlov Roman G. Efremov Alexander S. Arseniev 《Cell Adhesion & Migration》2010,4(2):284-298
The interaction between transmembrane helices is of great interest because it directly determines biological activity of a membrane protein. Either destroying or enhancing such interactions can result in many diseases related to dysfunction of different tissues in human body. One much studied form of membrane proteins known as bitopic protein is a dimer containing two membrane-spanning helices associating laterally. Establishing structure-function relationship as well as rational design of new types of drugs targeting membrane proteins requires precise structural information about this class of objects. At present time, to investigate spatial structure and internal dynamics of such transmembrane helical dimers, several strategies were developed based mainly on a combination of NMR spectroscopy, optical spectroscopy, protein engineering and molecular modeling. These approaches were successfully applied to homo- and heterodimeric transmembrane fragments of several bitopic proteins, which play important roles in normal and in pathological conditions of human organism.Key words: bitopic proteins, transmembrane domain dimer, spatial structure, dynamics, protein-protein interactions, protein-membrane interactions, molecular modeling, NMR 相似文献
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107.
O. V. Bocharova A. S. Urban K. D. Nadezhdin E. V. Bocharov A. S. Arseniev 《Biochemistry. Biokhimii?a》2013,78(11):1263-1271
More than half of the mutations associated with familiar Alzheimer’s disease have been found in the transmembrane domain of amyloid precursor protein (APP). These pathogenic mutations presumably influence the APP transmembrane domain structural and dynamic properties and result in its conformational change or/and lateral dimerization. Despite much data about the pathogenesis of Alzheimer’s disease, the initial steps of the pathogenesis remain unclear so far. For the investigation of the molecular basis of Alzheimer’s disease, we selected amyloid precursor protein fragment APP671-726 containing the transmembrane and metal-binding domains. This fragment is the substrate of the γ-secretase complex whose abnormal activity leads to the formation of amyloidogenic Aβ42 peptides. This work for the first time describes a highly effective cell-free APP671-726 production method and improved method of bacterial synthesis. Both methods yield milligram quantities of isotope-labeled protein for structural study by high resolution NMR spectroscopy in membrane mimicking milieus. 相似文献
108.
Yu. A. Zolotarev E. V. Bocharov A. K. Dadayan I. E. Kasheverov M. N. Zhmak I. V. Maslennikov Yu. A. Borisov A. S. Arseniev N. F. Myasoedov V. I. Tsetlin 《Russian Journal of Bioorganic Chemistry》2000,26(9):527-531
Tritium-labeled α-conotoxin G1 with a molar radioactivity of 35 Ci/mmol and full biological activity (according to the binding
to nicotinic acetylcholine receptor) was obtained by the high-temperature solid-state catalytic isotope exchange (HSCIE).
The tritium distribution in the molecule of α-conotoxin G1 was revealed by3H NMR spectroscopy. Tritium was found in all amino acid residues except for the Asn4-Pro5-Ala6 fragment. The data on the comparative
reactivity of C-H bonds, theab initio quantum-chemical calculation of the hydrogen exchange reaction, and the information on the spatial structures of α-conotoxin
G1 in solution and in crystal state allowed us to establish that the reactivity of H atoms may be increased by their interaction
with the electron donor O and N atoms at the transition state of the HSCIE reaction. A decrease in the rate of the HSCIE reaction
could be caused by both a poor spatial accessibility of C-H bonds and a limited mobility of the peptide fragment containing
these bonds. 相似文献
109.
Bocharov EV Mineev KS Volynsky PE Ermolyuk YS Tkach EN Sobol AG Chupin VV Kirpichnikov MP Efremov RG Arseniev AS 《The Journal of biological chemistry》2008,283(11):6950-6956
Proper lateral dimerization of the transmembrane domains of receptor tyrosine kinases is required for biochemical signal transduction across the plasma membrane. The spatial structure of the dimeric transmembrane domain of the growth factor receptor ErbB2 embedded into lipid bicelles was obtained by solution NMR, followed by molecular dynamics relaxation in an explicit lipid bilayer. ErbB2 transmembrane segments associate in a right-handed alpha-helical bundle through the N-terminal tandem GG4-like motif Thr652-X3-Ser656-X3-Gly660, providing an explanation for the pathogenic power of some oncogenic mutations. 相似文献
110.
Remaley AT Thomas F Stonik JA Demosky SJ Bark SE Neufeld EB Bocharov AV Vishnyakova TG Patterson AP Eggerman TL Santamarina-Fojo S Brewer HB 《Journal of lipid research》2003,44(4):828-836
In order to examine the necessary structural features for a protein to promote lipid efflux by the ABCA1 transporter, synthetic peptides were tested on ABCA1-transfected cells (ABCA1 cells) and on control cells. L-37pA, an l amino acid peptide that contains two class-A amphipathic helices linked by proline, showed a 4-fold increase in cholesterol and phospholipid efflux from ABCA1 cells compared to control cells. The same peptide synthesized with a mixture of l and d amino acids was less effective than L-37pA in solubilizing dimyristoyl phosphatidyl choline vesicles and in effluxing lipids. In contrast, the 37pA peptide synthesized with all d amino acids (D-37pA) was as effective as L-37pA. Unlike apoA-I, L-37pA and D-37pA were also capable, although at a reduced rate, of causing lipid efflux independent of ABCA1 from control cells, Tangier disease cells, and paraformaldehyde fixed ABCA1 cells. The ability of peptides to bind to cells correlated with their lipid affinity. In summary, the amphipathic helix was found to be a key structural motif for peptide-mediated lipid efflux from ABCA1, but there was no stereoselective requirement. In addition, unlike apoA-I, synthetic peptides can also efflux lipid by a passive, energy-independent pathway that does not involve ABCA1 but does depend upon their lipid affinity. 相似文献