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111.
Alisdair M. Boag Michael R. Christie Kerry A. McLaughlin Harriet M. Syme Peter Graham Brian Catchpole 《PloS one》2015,10(11)
Canine hypoadrenocorticism likely arises from immune-mediated destruction of adrenocortical tissue, leading to glucocorticoid and mineralocorticoid deficiency. In humans with autoimmune Addison’s disease (AAD) or autoimmune polyendocrine syndrome (APS), circulating autoantibodies have been demonstrated against enzymes associated with adrenal steroid synthesis. The current study investigates autoantibodies against steroid synthesis enzymes in dogs with spontaneous hypoadrenocorticism. Coding regions of canine CYP21A2 (21-hydroxylase; 21-OH), CYP17A1 (17-hydroxylase; 17-OH), CYP11A1 (P450 side-chain cleavage enzyme; P450scc) and HSD3B2 (3β hydroxysteroid dehydrogenase; 3βHSD) were amplified, cloned and expressed as 35S-methionine radiolabelled recombinant protein. In a pilot study, serum samples from 20 dogs with hypoadrenocorticism and four unaffected control dogs were screened by radio-immunoprecipitation assay. There was no evidence of reactivity against 21-OH, 17-OH or 3βHSD, but five dogs with hypoadrenocorticism showed immunoreactivity to P450scc compared with controls. Serum samples were subsequently obtained from 213 dogs diagnosed with hypoadrenocorticism and 110 dogs from a hospital control population. Thirty control dogs were randomly selected to establish a threshold for antibody positivity (mean + 3 × standard deviation). Dogs with hypoadrenocorticism were more likely to be P450scc autoantibody positive than hospital controls (24% vs. 1.2%, respectively; p = 0.0016). Sex was significantly associated with the presence of P450scc autoantibodies in the case population, with 30% of females testing positive compared with 17% of males (p = 0.037). Significant associations with breed (p = 0.015) and DLA-type (DQA1*006:01 allele; p = 0.017) were also found. This cross-sectional study indicates that P450scc autoantibodies are present in a proportion of dogs affected with hypoadrenocorticism. 相似文献
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114.
B Boag 《Journal of wildlife diseases》1988,24(3):450-455
European rabbits (Oryctolagus cuniculus) were collected monthly over a 10-yr period and the incidence of myxomatosis and the size of helminth populations were recorded. Myxomatosis occurred annually, always as an epidemic in the latter half of the year and was associated with both an increase in the percentage of animals infected and the size of the infections of the nematodes Trichostrongylus retortaeformis and Passalurus ambiguus and the cestode Mosgovoyia pectinata. It is suggested that myxomatosis had the effect of reducing the rabbits' overall immune response to infection and that due to the regular annual occurrence of myxomatosis, the resulting increase in the size of helminth infections must be considered an integral part of the population dynamics of these parasites. 相似文献
115.
Viable human diploid fibroblasts have been micro-encapsulated in EUDRAGIT RL, a commercially available water-insoluble polyacrylate, by an interfacial precipitation technique. Cells in medium and polymer solution (in diethyl phthalate) were coextruded and formed into droplets by a coaxial air stream. The droplets fell into a corn-oil/mineral-oil mixture to extract the solvent to precipitate the polymer around the cells. Capsules were ca. 500 mum in diameter depending on the air flowrate with a ca. 10-mum thick wall. When collagen (1 mg/mL) was added to the cell suspension prior to encapsulation and base-washed corn oil was used, cell growth occurred with one doubling achieved after five to six days as the collagen gel contracted inside the capsule. In the absence of collagen, cells spread on the inner wall of the capsule but did not grow, presumably because the surface charge on the capsule was inadequate. In similar fashion fibroblasts spread but did not grow on films of EUDRAGIT RL but did grow on blends of EUDRAGIT RL and EUDRAGIT E containing 10-30% of the latter more highly aminated polyacrylate. Although not suitable for anchorage-dependent cell growth by itself, EUDRAGIT RL has been suitable as a model polymer to demonstrate the feasibility of using water insoluble polyacrylates and organic solvents and nonsolvents for the micro-encapsulation of fibroblasts. Such microcapsules are of potential interest as a mode of large scale tissue culture for the production of novel therapeutic agents. 相似文献
116.
DNA fingerprinting reveals relation between tail ornaments and cuckoldry in barn swallows, Hirundo rustica 总被引:1,自引:0,他引:1
Smith Henrik G.; Montgomerie Robert; Poldman Tarmo; White Bradley N.; Boag Peter T. 《Behavioral ecology》1991,2(1):90-98
In an experimental study in Denmark, it was previously foundthat male barn swallows (Hirundo rustica) with elongated tailstreamers obtained an apparent fitness advantage through earlierpairing, an increased frequency of second clutches, and highertotal reproductive success per season. In a parallel study offive barn swallow colonies in Ontario, Canada, we also foundthat elongated males paired earlier and thus were apparentlypreferred by females. Now, using DNA fingerprinting on familiesfrom two of those Ontario colonies, we show that five elongatedmales fathered only 59% of the offspring in their nests, whereassix shortened males fathered 96% of their nestlings. Thus, althoughelongated males were clearly preferred by females at the timeof pair formation, tail elongation may have hampered the abilityof a male to guard his mate, resulting in an increase in extrapairfertilizations (EPFs). A significant negative correlation betweenthe number of EPFs and natural tail length in this experimentalstudy also suggests that tail streamer length may reflect malequality. (Behav Ecol 1990; 2: 9098) 相似文献
117.
Yves Plante Peter T. Boag Bradley N. White 《Evolution; international journal of organic evolution》1989,43(7):1522-1537
We examined mitochondrial-DNA (mtDNA) sequence heterogeneity on four adjacent trapping grids in an island population of meadow voles (Microtus pennsylvanicus) at two different population densities. Four restriction endonucleases revealed 20 different mtDNA composite phenotypes in samples totaling 198 meadow voles. There were significant heterogeneities in the distribution of four common mtDNA composite phenotypes among the four trapping grids, suggesting that there is population subdivision on a fine scale. Genetic distances between grids, mtDNA diversity within grids, and GST also varied during the study period. We found a decrease in genetic distance and an increase in diversity when the population density was high and vice versa when the population density was low. When population density was high, the coefficient of gene differentiation was smaller than the same coefficient observed when the population density was low. These changes in population subdivision and diversity are consistent with theoretical expectations of population structure in which effective female population size and dispersal are the critical variables. The data also support the hypothesis of maintenance of mtDNA diversity by population subdivision, rapid population growth rate, and dispersal. 相似文献
118.
Daniel Carpenter Emma Sherlock David T. Jones Jim Chiminoides Thomas Writer Roy Neilson Brian Boag Aidan M. Keith Paul Eggleton 《Biodiversity and Conservation》2012,21(2):475-485
Earthworms are important soil organisms yet we have limited knowledge on the geographical distribution of species. Using data
from a variety of sources representing a total of 3,941 locality records we have produced the first distribution maps of earthworm
species in the British Isles The maps highlight the paucity of knowledge on this ecologically important group. A systematic
approach needs to be taken to bring earthworm species data to a level comparable with other important invertebrate groups
such as nematodes and isopods. Through the recent establishment of an earthworm recording scheme, the Earthworm Society of
Britain, working with the Biological Records Centre and earthworm researchers across the British Isles, aim to build comprehensive
distribution information for future monitoring and research purposes. 相似文献
119.
Simon A. F. Darroch Erik A. Sperling Thomas H. Boag Rachel A. Racicot Sara J. Mason Alex S. Morgan Sarah Tweedt Paul Myrow David T. Johnston Douglas H. Erwin Marc Laflamme 《Proceedings. Biological sciences / The Royal Society》2015,282(1814)
The latest Neoproterozoic extinction of the Ediacara biota has been variously attributed to catastrophic removal by perturbations to global geochemical cycles, ‘biotic replacement’ by Cambrian-type ecosystem engineers, and a taphonomic artefact. We perform the first critical test of the ‘biotic replacement’ hypothesis using combined palaeoecological and geochemical data collected from the youngest Ediacaran strata in southern Namibia. We find that, even after accounting for a variety of potential sampling and taphonomic biases, the Ediacaran assemblage preserved at Farm Swartpunt has significantly lower genus richness than older assemblages. Geochemical and sedimentological analyses confirm an oxygenated and non-restricted palaeoenvironment for fossil-bearing sediments, thus suggesting that oxygen stress and/or hypersalinity are unlikely to be responsible for the low diversity of communities preserved at Swartpunt. These combined analyses suggest depauperate communities characterized the latest Ediacaran and provide the first quantitative support for the biotic replacement model for the end of the Ediacara biota. Although more sites (especially those recording different palaeoenvironments) are undoubtedly needed, this study provides the first quantitative palaeoecological evidence to suggest that evolutionary innovation, ecosystem engineering and biological interactions may have ultimately caused the first mass extinction of complex life. 相似文献
120.
Peter J. Van Coeverden de Groot Andrea S. Putnam Peter Erb Candace Scott Don Melnick Colleen O’Ryan Peter T. Boag 《Conservation Genetics》2011,12(3):783-792
Poaching and habitat destruction across sub-Saharan Africa brought the black rhinoceros (Diceros bicornis) close to extinction. Over the past few decades, however, one of four subspecies, D. b. bicornis, has experienced a significant population increase as a consequence of its protection within Etosha National Park (ENP), Namibia. We report here on the level and spatial distribution of black rhinoceros genetic diversity within ENP. Using nine microsatellite loci, genetic variation was assessed from 144 individuals. Our results are consistent with the observation of lower levels of genetic diversity in D. b. bicornis, when compared to D. b. michaeli, but greater diversity when compared to D. b. minor. We also showed that ENP’s black rhino genetic diversity is well represented in Waterberg National Park, originally founded with ENP individuals. We found no genetic signature of a recent bottleneck in ENP, however, suggesting that the genetic diversity within ENP has not been adversely affected by the recent severe population decline. Using Bayesian clustering methods, we observed no significant population structure within ENP, but positive spatial genetic correlation is observed at distances up to 25 km. This relationship exists in females but not males, suggesting reduced dispersal among females, the first evidence of limited female dispersal or philopatry in any species of rhinoceros. 相似文献