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151.
Partial sequencing and mapping of clones from two maize cDNA libraries 总被引:11,自引:0,他引:11
152.
Expression of mouse metallothionein-I gene confers cadmium resistance in transgenic tobacco plants 总被引:8,自引:0,他引:8
Aihua Pan Meizhu Yang Feng Tie Lingyua Li Zhangliang Chen Biggen Ru 《Plant molecular biology》1994,24(2):341-351
Transgenic tobacco plants containing a mouse metallothionein-I (MT-I) gene fused to the cauliflower mosaic virus 35S (CaMV 35S) promoter and nopaline synthase (nos) polyadenylation site were obtained by transforming tobacco leaf discs with an Agrobacterium tumefaciens strain carrying the chimaeric gene. Transformants were directly selected and rooted on medium containing cadmium and kanamycin. A total of 49 individual transgenic tobacco plants were regenerated. Among them 20% showed a very high expression level and their growth was unaffected by up to 200 M cadmium, whereas the growth of control plants was severely affected leaf chlorosis occurred on medium containing only 10 M cadmium. The concentration of MT-I in leaves of control and transgenic tobacco was determined with Cd/haemoglobin saturation assay, a polarographic method and western blotting. In addition, seeds from self-fertilized transgenic plants were germinated on medium containing toxic levels of cadmium and scored for tolerance/susceptibility to this heavy metal. The ratio of tolerant to susceptible plants was 3:1 indicating that the metallothionein gene is inherited as a single locus. 相似文献
153.
Jin Feng Kunitoshi Yamanaka Hironori Niki Teru Ogura Sota Hiraga 《Molecular & general genetics : MGG》1994,243(2):136-147
The nucleotide sequence was determined of the region upstream of the mukB gene of Escherichia coli. Two new genes were found, designated kicA and kicB (killing of cell); the gene order is kicB-kicA-mukB. Promoter activities were detected in the regions immediately upstream of kicB and kicA, but not in front of mukB. Gene disruption experiments revealed that the kicA disruptant was nonviable, but the kicB-disrupted mutant and the mutant lacking both the kicB and kicA genes were able to grow. When kicA disruptant cells bearing a temperature-sensitive replication plasmid carrying the kicA
+ gene were grown at 30° C and then transferred to 42° C, the mutant cells gradually lost colony-forming ability, even in the presence of a mukB
+ plasmid. Rates of protein synthesis, but not of RNA or DNA synthesis, fell dramatically during incubation at 42° C. These results suggested that the kicB gene encodes a killing factor and the kicA gene codes for a protein that suppresses the killing function of the kicB gene product. It was also demonstrated that KicA and KicB can function as a post-segregational killing system, when the genes are transferred from the E. coli chromosome onto a plasmid. 相似文献
154.
Lipase-catalyzed alcoholysis of triglycerides for the preparation of 2-monoglycerides 总被引:11,自引:0,他引:11
Anna Millqvist Patrick Adlercreutz Bo Mattiasson 《Enzyme and microbial technology》1994,16(12):1042-1047
Rhizopus arrhizus lipase immobilized on celite was used to prepare isomerically pure 2-monoglycerides by alcoholysis of triglycerides in organic media. Reaction parameters such as choice of solvent, choice of alcohol, and alcohol concentration were studied. When 12.5 mM tripalmitin was used as substrate, methyl-tert-butyl ether was the best solvent for alcoholysis at water activity 0.11. Ethanol gave the highest yield (97%) at an optimal ethanol concentration of 200–300 mM. At higher alcohol concentrations, the enzyme activity was substantially lowered. The enzyme preparation showed high stability in repeated-batch reactions. 相似文献
155.
华中地区小家鼠生物学特性观察 总被引:8,自引:2,他引:6
华中地区农村小家鼠的个体较小,主要栖息于农舍,终年繁殖,繁殖高峰期为7月和8月,1-3月为繁殖低谷期;种群数量高峰在9月,低谷期在7-8月,冬季的种群数量水平较高,呈后峰型。在较高的密度条件下,雌鼠的怀孕率降低,性成熟延缓。 相似文献
156.
157.
Largepterosaurwithamaximumwingspanofmorethan5metres.Theskullislowerandlonger,withoutmiddlecrestorsupraoccipita1crest.ThenasaIandpre-orbita1fenestraareconfluentcompletely,andoccupyaboutha1foftheskulI1ength.Thetoothlessbeakisslenderandpointed.The1ongneckiscomposedbyse-venslendercervicalvertebrae.Thenotariumconsistsofsixco-ossifiedanteriordorsalvertebrae-ThetaiIisextremlyshort.lthassixpairsof"A"shapegastralia.Theanteriorlimbsarestrong;thehumerusarethickandshort;thewing-metacarpalbonearelongert… 相似文献
158.
159.
广东封开县罗沙岩洞穴遗址第一期发掘简报 总被引:8,自引:1,他引:7
封开罗沙岩第一期发掘,出土了一些打制石器,它们有明确的地层,并有绝对年代测定数据,有助于解决广东是否有旧石器文化的争论。 相似文献
160.
A. S. Feng W. -Y. Lin L. Sun 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1994,175(5):531-546
Physiological studies were carried out in the frog (Rana pipiens pipiens) eighth nerve to determine: (i) whether the modulation rate or the silent gap was the salient feature that set the upper limit of time-locking to pulsed amplitude-modulated (PAM) stimuli, (ii) the gap detection capacity of individual eighth nerve fibers. Time-locked responses of 79 eighth nerve fibers to PAM stimuli (at the fiber's characteristic frequency) showed that the synchronization coefficient was a low-pass function of the modulation rate. In response to PAM stimuli having different pulse durations, a fiber gave rise to non-overlapping modulation transfer functions. The upper cut-off frequency of time locking was higher when tonepulses in PAM stimuli had shorter duration. The fact that the cut-off frequency was different for the different PAM series suggested that the AM rate was neither the sole, nor the main, determinant for the decay in time-locking at high AM rates. Gap detection capacity was determined for 69 eighth nerve fibers by assessing fiber's spiking activities to paired tone-pulses during an OFF-window and an ON-window. It was found that the minimum detectable gap of eighth nerve fibers ranged from 0.5 to 10 ms with an average of 1.23–2.16 ms depending on the duration of paired tone pulses. For each fiber, the minimum detectable gap was longer when the duration of tone pulses comprising the twin-pulse stimuli was more than four times longer. When the synchronization coefficient was plotted against the silent gap between tones pulses in the PAM stimuli, the gap response functions of a fiber as derived from multiple PAM series were equivalent to gap response functions deriving from twin-pulse series suggesting that it was the silent gap which primarily determined the upper limit of time-locking to PAM stimuli.Abbreviations
MTF
modulation transfer function
-
PAM
pulse amplitude modulated
-
SAM
sinusoidally amplitude modulated
-
SC
synchronization coefficient
-
TW
time window 相似文献