Transmission characteristics of Spiroplasma citri and its effect on leafhopper vectors from the Circulifer tenellus complex

Several leafhopper variants of the Circulifer tenellus complex were collected in “citrus stubborn” affected areas in Israel. Two of these variants transmitted the Spiroplasma citri to Matthiola incana after being injected with the disease agent. The variant from Atriplex halimus was designated Circulifer tenellus-A (CTA) and the variant from Portulaca oleracea was designated Circulifer tenellus-? (CTP). Transmission characteristics were determined for both leafhoppers. A high rate of transmission (43.3%) was obtained by single CTA leafhoppers that were injected with the Amiad S. citri isolate from the Upper Galilee, compared with 7% transmission obtained with the CTP leafhoppers. The Gilgal S. citri isolate from the Jordan Valley, was not transmitted by either. Injection was more effective than acquisition access feeding to render the leafhopper infective for both CTA and CTP. The minimum acquisition access period needed for the CTA variant to transmit the Amiad isolate was 1 h. Longer AAPs did not necessarily result in a higher rate of transmission. The minimum incubation period was 6 days and the maximum was 32 days. The LP₅₀ calculated from the logarithmic curve y = 45.74Ln(x)–53.68 was 9.64 days. The minimum inoculation access period (IAP) was lh. The same transmission parameters for the CTP variant could not be determined, as no transmission was obtained even when groups of five-six insects were placed on a single plant.     

A Comparative Study on the Effects of H2S and SO2 Fumigation on the Growth and Accumulation of Sulphate and Sulphydryl Compounds in Trifolium pratense L., Glycine max Merr. and Phaseolus vulgaris L.

Maas, F. M., De Kok, L. J., Peters, J. L. and Kuiper, P. J.C. 1987. A comparative study on the effects of H₂S and SO₂ fumigationon the growth and accumulation of sulphate and sulphydryl compoundsin Trifolium pratense L., Glycine max Merr. and Phaseolus vulgarisL.—J. exp. Bot. 38: 1459-1469. The effects of 0—25 mm³ dm³ H₂S and SO₂ on growth andsulphur content of shoots of Trifolium pratense, Glycine maxand Phaseolus vulgaris were studied. After 2 weeks of fumigationthe yield of T. pralense was reduced by 32% by H₂S, but notaffected by SO₂. Yield of G. max was not affected by H₂S, butreduced by 20% by SO₂, whereas that of P. vulgaris was increasedby 11% by H₂S and not affected by SO₂. Increases in sulphydrylcontent were already observed after 24 h of exposure to H₂Sand SO₂ in all plants. The increase was greatest in T. pratenseand smallest in P. vulgaris and, except for T. pratense, alwaysgreater in the H₂S-exposed than the SO₂-exposed plants. Oneday of exposure resulted in an increase in sulphate contentonly in the SO₂-fumigated plants, with the highest accumulationin T. pratense and the lowest in P. vulgaris. After 2 weeksan increase in sulphate content was also observed in the H₂S-exposedplants. This increase was also highest in T. pratense and lowestin P. vulgaris. Transpiration rate was not affected by a 24 h exposure to H₂Sor SO₂ and was highest in T. pratense, intermediate in G. maxand lowest in P. vulgaris. The order of theoretical rates of deposition of H₂S and SO₂correlated with the observed increases in sulphydryl contentduring the first 24 h of exposure in both H₂S and SO₂-fumigatedplants and with the increase in sulphate content in the SO₂-exposedplants. The increases in sulphydryl content were only 8% ofthe theoretical H₂S and SO₂-deposition fluxes, whereas sulphateaccumulation accounted for at least 57% of the theoretical SO₂-depositionflux. Key words: Air pollution, clover, French bean, Glutathione, Soybean, sulphur metabolism.     

The construction of a simultaneous functional order in nervous systems

In a previous paper (Part I) we introduced a model that constructs a simultaneous functional order in a set of neuronal elements by monitoring the coincidences in their signal activities (the so-called coincidence-model). The simultaneous signal activity in a neural net will be constrained both by its physical restrictions and by environmental constraints. In this paper we present the results of simulation experiments that were performed to study the influence of environmental constraits on the resulting functional order in a set of neural elements corresponding to a onedimensional detector array. We show that the coincidence-model produces a functional order that encodes the physical constraints of the environment. Moreover, we demonstrate that the signal activity in the neural net (the perceptions) can be related to events in the outer world. We provide some examples to demonstrate that our model may prove useful to gain insight into certain developmental disorders.     

Water Balance in Cucumis Plants, Measured by Nuclear Magnetic Resonance, I

In this paper we demonstrate the study of plant water balanceby the non-invasive measurement of tissue water content andwater flow using proton nuclear magnetic resonance (NMR). Sapvelocity and flux were measured independently in the presenceof an excess of stationary tissue water. The instrumentationdescribed allows automated and unattended measurement of flow-and water content-variables in a well-defined region of theplant over periods of several days, with a time resolution betweensuccessive measurements of c. 5 s. Using this apparatus theeffect of changes in light intensity (day/night rhythm) andrelative humidity on stem tissue water content as well as onthe velocity and flux of xylem sap in the stem were investigatedin a cucumber plant. The results are in agreement with predictionsfrom a simple model for plant water balance, which is basedon water potential, flow rate and resistance to flow. As longas only transpiration is varied, flow rate and water content(or potential) are affected in opposite ways as demonstratedin this paper. In contrast, the model predicts that changesin uptake (resulting from changes in, for example, root resistance)will induce changes in water content and flow in the same direction.An experimental verification of this prediction is given ina subsequent paper, where, in addition, the NMR results arecompared to those obtained with a dendrometer. Key words: Water balance model, Cucumis sativus L., flow, water content, NMR, water balance measurement     

Stomatal Response of some Cultivated and Wild Tuber-bearing Potatoes in Warm Tropics as Influenced by Water Deficits

Leaf resistances of 14 cultivated potato genotypes (Solanumspp) and three tuber-bearing wild Solanum species were comparedwhen plants were grown under water stress at two tropical sitesFactors investigated were diurnal changes in leaf resistance,the effect of plant age, transient drought versus well-wateredconditions of potted and field-grown plants These measurementswere carried out in order to determine the stomatal behaviourof tuber-bearing genotypes and species Significant genotypic differences in leaf resistances were notedwithin the cultivated genotypes All genotypes had higher resistanceswhen water-stressed, but LT-7 appeared to have the lowest leafresistances Genetic differences in stomatal behaviour of tuber-bearingSolanum species were confirmed Abaxial stomatal resistancesof water-stressed plants of the species ranged between 1 74and 13 8 s cm^–1 Stomata of S chacoense were less affectedby drought (three-fold) than S tuberosum (four-fold) The greatesteffect was on S jungasense (five-fold) and on S raphanifoliumThese data show that stomata behaviour among tuber-bearing Solanumspecies is sufficiently different to warrant investigationsof drought-resistance in potato species under dry hot conditions Solanum tuberosum L., Solanum raphanifolium, Solanum chacoense, Solanum jungasense, leaf resistance     

A New Procedure for the Calculation of Oxygen Diffusion Resistance in Legume Nodules from Flow-through Gas Analysis Data

Plants of white lupin (Lupinus albus L cv Multolupa) and soyabean(Glycine max L cv Clarke) were grown in controlled-environmentcabinets, subjected to various stresses and their nodular nitrogenaseactivity and total root respiration measured When these measurementswere used to calculate nodular oxygen diffusion resistance,using a simplified equation for Fick's first law of diffusion,it was found that the apparent resistance of stressed nodulesincreased anomalously with decreases in external oxygen concentrationA new analysis procedure is proposed to alleviate this anomalyThis procedure also uses the simplified Fick's law equationbut includes a respiratory contribution to the total oxygenflux across the diffusion barrier which is not coupled to nitrogenaseactivity Also, resistance is modelled as an exponential functionof external oxygen concentration Use of this analysis procedureproduces realistic values for total resistance and providesa characterisation of this resistance into a minimum value andan adjustment factor for changes in external oxygen It is postulatedthat the additional respiration component represents the activityof nodule cortex cells involved in the diffusion barrier, particularlythat of vascular bundles Oxygen diffusion resistance, nodule, nitrogen fixation, respiration