Role of normal microflora in the treatment of vibrio carrier state in rat gnotobionts]

Materials on the study of interactions between Vibrio cholera El Tor and some indigenous bacteria of human intestinal tract, which proved to be active antagonists in vitro, are presented in this work. Experiments were conducted on gnotobiotic rats (Fisher), using various combinations and variants of the order of the microorganism administration; there were revealed no antagonistic relations between Virbrio cholerae El Tor and Escherichia coli M-17, Lactobacillus fermenti, Lactobacillus plantarum. Vibrio cholera El Tor disappeared completely from the intestinal tract of the rats after its contact with the fecal microflora of healthy rats.     

Comprehensive Longitudinal Study of the Physical and Psychophysiological Development of Schoolchildren at the Preadolescent, Early Adolescent, and Late Adolescent Ontogenetic Stages

Results are presented of a 10-year comprehensive study of the changes in somatic, psychophysiological, and autonomic parameters of 132 students of an innovative school (a linguistics-oriented gymnasium) by using computer-aided psychophysiological and functional diagnostics. Certain changes were found in the development of the structural and functional psychosomatic complexes of students at different stages of their ontogenesis.     

Osteoinductive Properties of Secretome of Human Mesenchymal Stem Cells,Obtained with Automatic Cell Culture System

Cell and Tissue Biology - The secretome of human mesenchymal stem cells (MSCs) after osteogenic differentiation of FetMSC cells in vitro was obtained to develop tissue engineering technologies for...     

Lysosomal enzymes and natural killer activity

The natural killer activity (NKA) of human mononuclear cells and the activity of the lysosomal enzymes of these cells (arylsulfatase, acid phosphatase and beta-glucuronidase) has been studied in norm and under human lung cancer. The mononuclear cells were isolated from peripheral blood of 10 healthy donors and 20 patients with lung cancer of II-III stages. Under the action of mononuclear cells on the target cells (human erythroleukosis cells K-562 labeled with 3H-uridine) the NKA of mononuclear cells of patients was seen to decrease (cytotoxic index = 54.8 +/- 6.4%), in comparison with that of healthy donors (cytotoxic index = 65.1 +/- 4.5%). Simultaneously a decrease in arylsulfatase activity (0.05 +/- 0.01 nmoles/10(6) cells/min) was found in comparison with the control value (0.11 +/- 0.01 nmoles/10(6) cells/min). In 2-3 weeks after the operation the NKA value (cytotoxic index = 50.2 +/- 5.8%) was restored and arylsulfatase activity (0.09 +/- 0.02 nmoles/10(6) cells/min) was increased. There was no correlation between the NKA value and the activities of acid phosphatase and beta-glucuronidase. The parallelism observed between changes in NKA value and arylsulfatase activity may suggest a possible participation of this enzyme in the killing mechanism at the stage of cerebroside sulfate ester degradation of the target cell membrane to initiate the lytic events.     

Live enteroviral vaccines for the emergency nonspecific prevention of mass respiratory diseases during fall-winter epidemics of influenza and acute respiratory diseases]

The results of the 3-year controlled trials of a new method of nonspecific urgent prophylaxis of influenza and acute respiratory diseases (ADR) by immunization of healthy adults with standard live enterovirus oral vaccines, introduced in 2-3 administrations at intervals of 7-10 days, at the initial stages of autumn and winter epidemics are presented. Observations, carried out in three republics, covered more than 150,000 persons immunized with enterovirus interferonogenic vaccines. A considerable decrease in morbidity rate among the vaccinees was achieved (on the average, by 3.2 times) in comparison to that among nonimmunized subjects. The method of nonspecific prophylaxis with live enterovirus interferonogenic vaccines is recommended during outbreaks of diseases induced simultaneously by several causative agents of influenza and ARD, as well as by pathogenic enterovirus strains.