DNA evolutionary rates in nine-primaried passerine birds

Differences in single-copy nuclear-DNA sequences among 13 species of passerine birds were measured using DNA-DNA hybridization. A matrix of pairwise dissimilarity values (delta mode distances) was constructed from analysis of fitted thermal dissociation curves. A least-squares method of phylogenetic estimation was used to construct two topologies from the distance matrix, one constraining branch lengths of sister taxa to be equal and the other permitting such lengths to vary. These topologies were identical in the pattern of branching of taxa, and the difference in their sums of squares was not statistically significant, suggesting that rates of DNA evolution in sister groups of nine- primaried oscines are equal. A nonparametric test for nonrandom variation in distances of sister groups to outgroup taxa revealed no statistically significant deviation from random variation that would be expected as a result of measurement error. However, the level of measurement error was such that rates of DNA evolution in sister taxa could vary by as much as 10% without being detected with the statistical methods used here.      

Quest for wine yeasts—An old story revisited

Numerous studies have described the yeast biota of grapes, and grape must in order to understand better the succession of yeasts during fermentation of wine. The origin of the wine yeasts has been rather controversial. By using more elaborate isolation methods, classical genetic analysis and electrophoretic karyotyping of monosporic clones, with this study, credible proof now exists that the vineyard is the primary source for the wine yeasts and that strains found on the grapes can be followed through the fermentation process.     

The environmental significance of algae in the mi Llandovery succession of the central Oslo Region

Girvanella , micritization phenomena and the dasycladacean alga Cyclocrinites are found in the middle Llandovery succession of the central Oslo Region. Both Girvanella and micritization phenomena occur in carbonate-dominated sequences where sedimentary structures and associated faunas suggest depositional environments above normal wave base. In contrast, Cyclocrinites occurs in shale-dominated sequences where other evidence suggests quiet, deep-water environments. We suggest that Cyclocrinites would not normally be preserved in situ in its life environment in relatively turbulent shallow waters. Preservation most commonly resulted from post mortem transport into low-energy muddy environments devoid of in situ algal activity. The resultant fossil is a flattened cast of this originally spherical alga.     

Behavioral budgets of captive sea otter mother–pup pairs during pup development

Instantaneous sampling of eight behavior categories for a captive mother sea otter (Enhydra lutris) and her four pups was conducted continuously during the first three months following each pup's birth. Initially, pup-grooming and rest occupied the largest proportion of the mother's time. Despite a steady decline in the amount of time the mother spent pup-grooming, the mother continued to groom the pup a substantial amount of time three months after the pup's birth. The proportion of time the mother spent resting, self-grooming, or swimming did not change significantly over the study period, suggesting that these behaviors are important to the mother's self-maintenance. The newborn pups' behaviors were initially limited to rest, active, and nursing, with large proportions of time spent resting and nursing. A significant increase in self-grooming occurred regularly by week 2, followed by increases in swimming (week 3), diving (week 6), and feeding (week 7). Three months after birth, the pups' behavior budgets were similar to their mother, with the exception that pups self-groomed substantially less than their mother self-groomed. The lower proportion of pup self-grooming may be due to the continued close proximity to their mother in captivity, which afforded extended association, and allowed the mother to continue to perform this complex behavior for the pup. © 1993 Wiley-Liss, Inc.     

Disasters collide at the intersection of extreme weather and infectious diseases

Natural disasters interact to affect the resilience and prosperity of communities and disproportionately affect low income families and communities of colour. However, due to lack of a common theoretical framework, these are rarely quantified. Observing severe weather events (e.g. hurricanes and tornadoes) and epidemics (e.g. COVID-19) unfolding in southeastern US communities led us to conjecture that interactions among catastrophic disturbances might be much more considerable than previously recognized. For instance, hurricane evacuations increase human aggregation, a factor that affects the transmission of acute infections like SARS-CoV-2. Similarly, weather damage to health infrastructure can reduce a community's ability to provide services to people who are ill. As globalization and human population and movement continue to increase and weather events are becoming more intense, such complex interactions are expected to magnify and significantly impact environmental and human health.     

BOOTSTRAP CORRECTION FOR DIURNAL ACTIVITY CYCLE IN CENSUS DATA FOR ANTARCTIC SEALS1

Antarctic phocid seals and particularly the crabeater (Lobodon carcinophagus) have been observed to display a diurnal cycle in their propensity to haul out on pack ice where they are visible for census. The fact that they are not visible for much of the 24-h period means that density estimates made over broad geographic areas at various times of the day statistically confound this cycle with geographic variability. Limitation of census observations to times of peak haulout results in extreme logistical difficulties and/or considerable reduction in sample size upon which to base population estimates. Reduced sample size results in high variability in population estimates and broad confidence bands. To develop a model with which to correct density estimates for variability due to diurnal cycle, a series of stationary censuses at fixed locations in the Antarctic continental ice pack was made over significant fractions of several days. A unimodal polynomial model for the observed density variation in any one location was statistically significant; a similar model combining multiple locations with densities standardized to peak daily values was also significant. The latter model was used to make corrections for time of day to density estimates in three test data sets taken over broad geographic areas of the Antarctic. Statistical simulation (bootstrap) methods were used to determine if variances of corrected density estimates were lower than those based on uncorrected observations taken only during the peak haulout times of the day. Results were that 95% interval estimates for corrected densities were narrowed to between 40% and 61% of the uncorrected estimates. While there are additional possible sources of variation in haulout tendency, pending further data collection and analyses, the model developed represents a considerably more precise methodology than either averaging over haulout variability or limiting observations to peak daily periods.     

A quantitative assessment of congruence between molecular and nonmolecular estimates of phylogeny

Summary An important means of assessing the validity of phylogenetic hypotheses is to measure congruence between different studies of the same group. We applied statistical methods to assess patterns of congruence among phylogenies taken from the literature, using strict-consensus and quartet statistics to measure congruence for 48 pairs of phylogenies of various groups of birds and mammals. The strict-consensus measures were higher on average for distance-distance comparisons than for distance-parsimony or parsimony-parsimony comparisons, and for molecular-molecular comparisons than for molecular-morphological or morphological-morphological comparisons. However, the only factor that was consistently important statistically was the number of taxa involved in the comparison, with congruence decreasing as the number of taxa increased. Quartet indices, which measure network similarity regardless of root position, also showed an effect based on number of taxa but exhibited no trend toward higher congruence in molecular-molecular and distance-distance comparisons. The number of studies with jointly unresolved quartets was small, indicating that data sets varied in the degree to which they could resolve relationships at different phylogenetic levels.The levels of congruence between phylogenies, including those employing single-copy nuclear DNA hybridization data, appear to be higher than expected in random sets of trees, cannot be explained by nonindependence of data sets, and thus provide empirical support for the validity of both distance and parsimony methods of phylogenetic inference. In specific instances, low congruence pointed to mistakes in the application of certain methods, and to the existence of problem taxa in need of additional study.     

Insecticidal effects of selected biological control agents on the larvae of Plutella xylostella (Lepidoptera: Plutellidae)

To identify a more effective and safe biological control agent against a common cabbage pest, Plutella xylostella (L.) (Lepidoptera: Plutellidae), the insecticidal effects of selected biological agents were evaluated. The highest insecticidal effects determined were 100, 73.5, 45.5, 47 and 55.3% using toxin HD‐1 (isolated from the Harry Dumagae strain of Bacillus thuringiensis), toxin BTS‐1 (isolated from the tenebrionis strain of B. thuringiensis), B. thuringiensis Berliner, B. thuringiensis israelensis and B. thuringiensis kurstaki, respectively.     

Effects of four Bacillus spp. of soil origin on the Colorado potato beetle Leptinotarsa decemlineata (Say)

Four species of Bacillus were isolated from soil in an effort to find safe, effective and alternative biological control agents against plant pests. These bacteria were identified as Bacillus pumilus, Bacillus sphaericus, Bacillus megaterium and Bacillus cereus on the basis of fatty acid methyl ester analysis and carbon utilization profiles by using Microbial Identification and Biolog Microplate Systems. Laboratory experiments carried out to determine the insecticidal activities of these isolates showed that B. pumilus caused 95.7 and 26.7% mortality and B. sphaericus caused 74.5 and 23.3% mortality of Leptinotarsa decemlineata larvae and adults, respectively. B. cereus and B. megaterium showed 51.1 and 29.7%, respectively, of L. decemlineata larvae. This study presents at least two Turkish isolates from the genus Bacillus showing high insecticidal activity against L. decemlineata.     

A ligand-mediated hydrogen bond network required for the activation of the mineralocorticoid receptor

Ligand binding is the first step in hormone regulation of mineralocorticoid receptor (MR) activity. Here, we report multiple crystal structures of MR (NR3C2) bound to both agonist and antagonists. These structures combined with mutagenesis studies reveal that maximal receptor activation involves an intricate ligand-mediated hydrogen bond network with Asn770 which serves dual roles: stabilization of the loop preceding the C-terminal activation function-2 helix and direct contact with the hormone ligand. In addition, most activating ligands hydrogen bond to Thr945 on helix 10. Structural characterization of the naturally occurring S810L mutant explains how stabilization of a helix 3/helix 5 interaction can circumvent the requirement for this hydrogen bond network. Taken together, these results explain the potency of MR activation by aldosterone, the weak activation induced by progesterone and the antihypertensive agent spironolactone, and the binding selectivity of cortisol over cortisone.