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排序方式: 共有149条查询结果,搜索用时 15 毫秒
101.
Chopra SL Blackwood AC Dale DG 《Canadian journal of comparative medicine and veterinary science》1963,27(12):290-294
Studies were conducted on the enumeration of 7 groups of fecal microflora including total aerobes, total anaerobes, coliforms, lactobacilli, staphylococci, streptococci and yeasts and molds of 18-day old piglets. These pigs were early weaned (21 days) on different modifications of an early-weaning ration. The above mentioned microflora were enumerated again when some of the pigs in a replicate started scouring. The occurrence of diarrhea was always associated with significant increases in the numbers of coliforms and corresponding decreases in the lactobacilli counts. No other single group of fecal microflora differed in the scouring and non-scouring animals. The composition of the early-weaning ration offered to the animals did not, in itself, influence the fecal microflora to any appreciable extent. In another series of experiments, enumeration of coliforms and lactobacilli was conducted on samples obtained from different segments of the intestinal tracts of scouring and non-scouring pigs. Increased numbers of coliforms and decreased numbers of lactobacilli were observed at all levels of the intestinal tracts of the scouring animals. However, these changes were more marked in the duodenal samples than in those obtained from other parts of the intestine. 相似文献
102.
Modification of BRCA1-associated breast cancer risk by the polymorphic androgen-receptor CAG repeat 总被引:16,自引:0,他引:16
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Rebbeck TR Kantoff PW Krithivas K Neuhausen S Blackwood MA Godwin AK Daly MB Narod SA Garber JE Lynch HT Weber BL Brown M 《American journal of human genetics》1999,64(5):1371-1377
Compared with the general population, women who have inherited a germline mutation in the BRCA1 gene have a greatly increased risk of developing breast cancer. However, there is also substantial interindividual variability in the occurrence of breast cancer among BRCA1 mutation carriers. We hypothesize that other genes, particularly those involved in endocrine signaling, may modify the BRCA1-associated age-specific breast cancer risk. We studied the effect of the CAG repeat-length polymorphism found in exon 1 of the androgen-receptor (AR) gene (AR-CAG). AR alleles containing longer CAG repeat lengths are associated with a decreased ability to activate androgen-responsive genes. Using a sample of women who inherited germline BRCA1 mutations, we compared AR-CAG repeat length in 165 women with and 139 women without breast cancer. We found that women were at significantly increased risk of breast cancer if they carried at least one AR allele with >/=28 CAG repeats. Women who carried an AR-CAG allele of >/=28, >/=29, or >/=30 repeats were given a diagnosis 0.8, 1.8, or 6.3 years earlier than women who did not carry at least one such allele. All 11 women in our sample who carried at least one AR-CAG allele with >/=29 repeats had breast cancer. Our results support the hypothesis that age at breast cancer diagnosis is earlier among BRCA1 mutation carriers who carry very long AR-CAG repeats. These results suggest that pathways involving androgen signaling may affect the risk of BRCA1-associated breast cancer. 相似文献
103.
Henry N Williams Despoina S Lymperopoulou Rana Athar Ashvini Chauhan Tamar L Dickerson Huan Chen Edward Laws Timkhite-Kulu Berhane Adrienne R Flowers Nadine Bradley Shanterial Young Denene Blackwood Jacqueline Murray Oladipupo Mustapha Cory Blackwell Yahsuan Tung Rachel T Noble 《The ISME journal》2016,10(2):491-499
Predation on bacteria and accompanying mortality are important mechanisms in controlling bacterial populations and recycling of nutrients through the microbial loop. The agents most investigated and seen as responsible for bacterial mortality are viruses and protists. However, a body of evidence suggests that predatory bacteria such as the Halobacteriovorax (formerly Bacteriovorax), a Bdellovibrio-like organism, contribute substantially to bacterial death. Until now, conclusive evidence has been lacking. The goal of this study was to better understand the contributors to bacterial mortality by addressing the poorly understood role of Halobacteriovorax and how their role compares with that of viruses. The results revealed that when a concentrated suspension of Vibrio parahaemolyticus was added into microcosms of estuarine waters, the native Halobacteriovorax were the predators that responded first and most rapidly. Their numbers increased by four orders of magnitude, whereas V. parahaemolyticus prey numbers decreased by three orders of magnitude. In contrast, the extant virus population showed little increase and produced little change in the prey density. An independent experiment with stable isotope probing confirmed that Halobacteriovorax were the predators primarily responsible for the mortality of the V. parahaemolyticus. The results show that Halobacteriovorax have the potential to be significant contributors to bacterial mortality, and in such cases, predation by Halobacteriovorax may be an important mechanism of nutrient recycling. These conclusions add another dimension to bacterial mortality and the recycling of nutrients. 相似文献
104.
Vibrio parahaemolyticus and Vibrio vulnificus in South America: water,seafood and human infections 总被引:3,自引:0,他引:3
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S.M. Raszl B.A. Froelich C.R.W. Vieira A.D. Blackwood R.T. Noble 《Journal of applied microbiology》2016,121(5):1201-1222
The bacterial species, Vibrio parahaemolyticus and Vibrio vulnificus, are ubiquitous in estuaries and coastal waters throughout the world, but they also happen to be important human pathogens. They are concentrated by filter‐feeding shellfish which are often consumed raw or undercooked, providing an important potential route of entry for an infective dose of these bacteria. Vibrio parahaemolyticus can cause abdominal cramping, nausea, diarrhoea, vomiting, chills and fever. Vibrio vulnificus can cause similar gastrointestinal‐related symptoms, but can also spread to the bloodstream, resulting in primary septicaemia, and it can also cause disease via wound infections. The objective of this article is to summarize, for the first time, the incidence and importance of V. parahaemolyticus and V. vulnificus in South America, in environmental waters and seafood, especifically molluscan shellfish, as well as human infection cases and outbreaks. It appears that infections from V. parahaemolyticus have been more strongly related to shellfish ingestion and have been more frequently reported on the Pacific coast of South America. Conversely, V. vulnificus has been more frequently acquired by water contact with open wounds and its presence has been more heavily reported along the Atlantic coast of South America, and while documented to cause serious mortality, have been relatively few in number. The impacts of El Nino Southern Oscillation (ENSO) have been observed to cause an increase in V. parahaemolyticus outbreaks on the Pacific coast of South America. The implementation of a regulated monitoring approach, along with the use of faster, more accurate and virulence‐specific detection approaches, such as PCR confirmation, should be considered to detect the presence of pathogenic Vibrio strains in environmental and seafood samples for protection of public health. Furthermore, improved clinical surveillance with suspected cases should be implemented. This review highlights the need for more research and monitoring of vibrios in South America, in water, shellfish and clinical samples. 相似文献
105.
106.
107.
Hasturk H Kantarci A Goguet-Surmenian E Blackwood A Andry C Serhan CN Van Dyke TE 《Journal of immunology (Baltimore, Md. : 1950)》2007,179(10):7021-7029
Resolvin E1 (RvE1) is a potent proresolving mediator of inflammation derived from omega-3 eicosapentaenoic acid that acts locally to stop leukocyte recruitment and promote resolution. RvE1 displays potent counter-regulatory and tissue-protective actions in vitro and in vivo. Periodontal disease is a local inflammatory disease initiated by bacteria characterized by neutrophil-mediated tissue injury followed by development of a chronic immune lesion. In this study, we report the treatment of established periodontitis using RvE1 as a monotherapy in rabbits compared with structurally related lipids PGE(2) and leukotriene B(4). PGE(2) and leukotriene B(4) each enhanced development of periodontitis and worsened the severity of disease. Promotion of resolution of inflammation as a therapeutic target with RvE1 resulted in complete restoration of the local lesion, and reduction in the systemic inflammatory markers C-reactive protein and IL-1beta. This report is the first to show that resolution of inflammation by a naturally occurring endogenous lipid mediator results in complete regeneration of pathologically lost tissues, including bone. 相似文献
108.
Although field studies have demonstrated an ecosystem-specific effect of experimental atmospheric nitrogen (N) deposition
on litter decomposition, a mechanistic understanding of how ligninolytic microbial communities respond to atmospheric deposition
is lacking. Because high levels of inorganic N suppress lignin decomposition by some basidiomycetes, it is plausible that
the abundance and activity of these key microorganisms underlies differential ecosystem responses of decomposition to atmospheric
N deposition. We hypothesize that: (a) atmospheric N deposition will cause an ecosystem-specific reduction in basidiomycete
activity and abundance with greatest decreases in ecosystems with lignin-rich forest litter and (b) the abundance of lignin
degrading basidiomycetes will be positively correlated with ligninolytic enzyme activity. To test these hypotheses, we measured
the effects of experimental N deposition on the potential activity of phenol oxidase enzymes, and the abundance of basidiomycete
genes encoding laccase, a primary phenol oxidase enzyme, in three hardwood forests spanning a range of leaf litter lignin
content. The black oak-white oak (BOWO) contains high lignin litter, the sugar maple-basswood (SMBW) has low lignin litter,
and the sugar maple-red oak (SMRO) is intermediate. An ecosystem by N deposition interaction significantly influenced phenol
oxidase activity in the surface soil (P = 0.05), where phenol oxidase activity decreased with increasing experimental N deposition in the BOWO ecosystem. No consistent
response to N deposition was evident for surface soil phenol oxidase activity within either the SMRO or SMBW ecosystem. This
interaction did not influence laccase gene abundance. Instead, basidiomycete laccase gene abundance was reduced by experimental
N deposition (main effect) in surface soil. There was only a weak correlation between basidiomycete laccase gene abundance
and potential phenol oxidase enzyme activity, suggesting that the abundance of organisms possessing laccase genes may not
control phenol oxidase activity in soil. Our results suggest that the regulation of laccase gene expression may mediate the
decomposition response to atmospheric N deposition. 相似文献
109.
Interpreting Ecological Diversity Indices Applied to Terminal Restriction Fragment Length Polymorphism Data: Insights from Simulated Microbial Communities 总被引:2,自引:1,他引:1
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Christopher B. Blackwood Deborah Hudleston Donald R. Zak Jeffrey S. Buyer 《Applied microbiology》2007,73(16):5276-5283
Ecological diversity indices are frequently applied to molecular profiling methods, such as terminal restriction fragment length polymorphism (T-RFLP), in order to compare diversity among microbial communities. We performed simulations to determine whether diversity indices calculated from T-RFLP profiles could reflect the true diversity of the underlying communities despite potential analytical artifacts. These include multiple taxa generating the same terminal restriction fragment (TRF) and rare TRFs being excluded by a relative abundance (fluorescence) threshold. True community diversity was simulated using the lognormal species abundance distribution. Simulated T-RFLP profiles were generated by assigning each species a TRF size based on an empirical or modeled TRF size distribution. With a typical threshold (1%), the only consistently useful relationship was between Smith and Wilson evenness applied to T-RFLP data (TRF-Evar) and true Shannon diversity (H′), with correlations between 0.71 and 0.81. TRF-H′ and true H′ were well correlated in the simulations using the lowest number of species, but this correlation declined substantially in simulations using greater numbers of species, to the point where TRF-H′ cannot be considered a useful statistic. The relationships between TRF diversity indices and true indices were sensitive to the relative abundance threshold, with greatly improved correlations observed using a 0.1% threshold, which was investigated for comparative purposes but is not possible to consistently achieve with current technology. In general, the use of diversity indices on T-RFLP data provides inaccurate estimates of true diversity in microbial communities (with the possible exception of TRF-Evar). We suggest that, where significant differences in T-RFLP diversity indices were found in previous work, these should be reinterpreted as a reflection of differences in community composition rather than a true difference in community diversity. 相似文献
110.
The effects of polluted and leached snowmelt waters on an upland soil bacterial community were determined in laboratory soil cores using simulated solutions. 24 isolate characters were determined before, and after, 32 days of solution application.The soil bacterial community exposed to polluted meltwaters (pH 2.3) showed a significant increase in its ability to metabolise the carbohydrates tested, and a significant decrease in the number of Gran-positive coccal forms. The percentage of Micrococcus isolates declined from 27% of the community to 2%, whilst Pseudomonas and Enterobacteriaceae isolates increased in number.The soil bacterial community exposed to leached snowmelt waters (pH 5.4) showed a significant increase in biochemical potential, but the ability to metabolise carbohydrates showed no change. 相似文献