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41.
Orzechowski Xavier M Pasqualotto AC Uchoa Sales Mda P Bittencourt Severo C Peixoto Camargo JJ Severo LC 《Revista iberoamericana de micología》2008,25(3):176-178
Invasive pulmonary aspergillosis is typically caused by a single Aspergillus species, most frequently Aspergillus fumigatus. Here we report that a lung transplant recipient developed invasive aspergillosis due to a mixed infection caused by Aspergillus flavus and A. fumigatus. The implications for this unusual finding are discussed. 相似文献
42.
Marcos Roberto de Oliveira Max William Soares Oliveira Guilherme Antônio Behr Matheus Augusto de Bittencourt Pasquali José Cláudio Fonseca Moreira 《Neurochemical research》2009,34(8):1410-1416
Vitamin A, beyond its biological role, is an alternative choice in treating some life threatening pathologies, for instance
leukemia and immunodeficiency. On the other hand, vitamin A therapy at moderate to high doses has caused concern among public
health researchers due to the toxicological aspect resulting from such habit. It has been described hepatotoxicity, cognitive
disturbances and increased mortality rates among subjects ingesting increased levels of vitamin A daily. Then, based on the
previously reported data, we investigated here receptor for advanced glycation endproducts (RAGE) immunocontent and oxidative
damage levels in cerebral cortex of vitamin A-treated rats at clinical doses (1,000–9,000 IU/kg day−1). RAGE immunocontent, as well as oxidative damage levels, were observed increased in cerebral cortex of vitamin A-treated
rats. Whether increased RAGE levels exert negative effects during vitamin A supplementation it remains to be investigated,
but it is very likely that deleterious consequences may arise from such alteration. 相似文献
43.
Chester Bittencourt Sacramento Vanessa Dionisio Cantagalli Mariana Grings Leonardo Pinto Carvalho José Carlos Costa Baptista‐Silva Abram Beutel Cassia Toledo Bergamaschi Ruy Ribeiro de Campos Junior Jane Zveiter de Moraes Christina Maeda Takiya Vívian Yochiko Samoto Radovan Borojevic Flavia Helena da Silva Nance Beyer Nardi Hans Fernando Dohmann Hamilton Silva Junior Valderez Bastos Valero Sang Won Han 《The journal of gene medicine》2009,11(4):345-353
44.
de Souza MI Salgueiro F Carnavale-Bottino M Félix DB Alves-Ferreira M Bittencourt JV Margis R 《Genetics and molecular biology》2009,32(3):546-556
Habitat fragmentation and a decrease in population size may lead to a loss in population genetic diversity. For the first time, the reduction in genetic diversity in the northernmost limit of natural occurence (southeastern Brazil) of Araucaria angustifolia in comparison with populations in the main area of the species continuous natural distribution (southern Brazil), was tested. The 673 AFLPs markers revealed a high level of genetic diversity for the species (Ht = 0.27), despite anthropogenic influence throughout the last century, and a decrease of H in isolated populations of southeastern Brazil (H = 0.16), thereby indicating the tendency for higher genetic diversity in remnant populations of continuous forests in southern Brazil, when compared to natural isolated populations in the southeastern region. A strong differentiation among southern and southeastern populations was detected (AMOVA variance ranged from 10%-15%). From Bayesian analysis, it is suggested that the nine populations tested form five "genetic clusters" (K = 5). Five of these populations, located in the northernmost limit of distribution of the species, represent three "genetic clusters". These results are in agreement with the pattern of geographic distribution of the studied populations. 相似文献
45.
Thiago Gomes Heck Sofia Pizzato Scomazzon Patrícia Renck Nunes Cinthia Maria Schöler Gustavo Stumpf da Silva Aline Bittencourt Maria Cristina Faccioni-Heuser Mauricio Krause Roberto Barbosa Bazotte Rui Curi Paulo Ivo Homem de BittencourtJr 《Cell stress & chaperones》2017,22(2):271-291
Exercise stimulates immune responses, but the appropriate “doses” for such achievements are unsettled. Conversely, in metabolic tissues, exercise improves the heat shock (HS) response, a universal cytoprotective response to proteostasis challenges that are centred on the expression of the 70-kDa family of intracellular heat shock proteins (iHSP70), which are anti-inflammatory. Concurrently, exercise triggers the export of HSP70 towards the extracellular milieu (eHSP70), where they work as pro-inflammatory cytokines. As the HS response is severely compromised in chronic degenerative diseases of inflammatory nature, we wondered whether acute exercise bouts of different intensities could alter the HS response of lymphocytes from secondary lymphoid organs and whether this would be related to immunoinflammatory responses. Adult male Wistar rats swam for 20 min at low, moderate, high or strenuous intensities as per an overload in tail base. Controls remained at rest under the same conditions. Afterwards, mesenteric lymph node lymphocytes were assessed for the potency of the HS response (42 °C for 2 h), NF-κB binding activity, mitogen-stimulated proliferation and cytokine production. Exercise stimulated cell proliferation in an “inverted-U” fashion peaking at moderate load, which was paralleled by suppression of NF-κB activation and nuclear location, and followed by enhanced HS response in relation to non-exercised animals. Comparative levels of eHSP70 to iHSP70 (H-index) matched IL-2/IL-10 ratios. We conclude that exercise, in a workload-dependent way, stimulates immunoinflammatory performance of lymphocytes of tissues far from the circulation and this is associated with H-index of stress response, which is useful to assess training status and immunosurveillance balance. 相似文献
46.
Flavia Maria Darcie Marquitti Paulo Roberto Guimarães Jr Mathias Mistretta Pires Luiz Fernando Bittencourt 《Ecography》2014,37(3):221-224
Many ecological systems can be represented as networks of interactions. A key feature in these networks is their organization into modules, which are subsets of tightly connected elements. We introduce MODULAR to perform rapid and autonomous calculation of modularity in network sets. MODULAR reads a set of files representing unipartite or bipartite networks, and identifies modules using two different modularity metrics widely used in the ecological networks literature. To estimate modularity, the software offers five optimization methods to the user. The software also includes two null models commonly used in studies of ecological networks to verify how the degree of modularity differs from two distinct theoretical benchmarks. 相似文献
47.
Perinotto WM Angelo IC Golo PS Quinelato S Camargo MG Sá FA Bittencourt VR 《Experimental parasitology》2012,130(3):257-260
This study aimed to evaluate the in vitro effect of the entomopathogenic fungi Metarhizium anisopliae sensu lato (s.l.) and Beauveria bassiana sensu lato (s.l.) on two distinct populations of Rhipicephalus microplus, from two different experimental farms. Bioassays were performed with engorged females, eggs and larvae. Fungal infection was evaluated based on biological parameters of treated engorged females, percentage of hatch from treated eggs, and percentage of mortality and mean lethal time (LT90) of treated larvae. When the treatments were compared between the two populations, there were significant differences in the following parameters: pre-oviposition period, hatching period and egg production index. Moreover, the results showed that B. bassiana s.l., isolate Bb 986, was more virulent than M. anisopliae s.l., isolate 959, for engorged females, showing a control percentage of 49%. In the bioassay with eggs, the hatching percentage ranged from 3.1% to 49.5% in one population and from 3.4% to 42.7% in the other, with no significant difference between the two populations. In the bioassay with unfed larvae, the mortality percentage ranged from 91.8% to 98.7% in one population and from 71.0% to 94.0% in the other. The LT(90) varied from 19.52 to 27.51 days in one of the populations and 22.89 to 37.31 days in the other. These results suggest that populations of R. microplus show distinct variation in their susceptibility to B. bassiana s.l. and M. anisopliae s.l. 相似文献
48.
49.
Laura Bedin Denardi Jéssica Tairine Keller Maria Isabel de Azevedo Vanessa Oliveira Fernanda Baldissera Piasentin Cecília Bittencourt Severo Janio Morais Santurio Sydney Hartz Alves 《Mycopathologia》2018,183(2):359-370
The checkerboard broth microdilution assay (BMD) is the most frequently used method for the in vitro evaluation of drug combinations. However, its use to evaluate the effect of antifungal drugs on filamentous fungi is sometimes associated with endpoint-reading difficulties, and different degrees of interaction are assigned to the same drug combination. We evaluated combinations of the azoles, itraconazole, posaconazole, and voriconazole, with the echinocandins, anidulafungin, caspofungin, and micafungin, against 15 itraconazole-resistant Aspergillus fumigatus clinical strains via the checkerboard BMD and Etest assay. Readings after 24 and 48 h, considering the two reading endpoints, the minimum inhibitory concentration (MIC) and minimum effective concentration (MEC), were performed for both methods. Our results showed that the correlation coefficients between the BMD and Etest methods were quite diverse to the drug combinations tested. The highest correlation coefficients of the Etest with the BMD assays (MEC and MIC reading) were the Etest-MIC reading at 24 h and the Etest-MEC reading at 48 h. Improvements in experimental conditions may increase the correlation between the two methods and ensure that Etest assay can be safely used in the evaluation of antifungal combinations against Aspergillus species. 相似文献
50.
SANDRINE BESSOU JEAN-ETIENNE SURLVE-BAZEILLE EVELYNE SORBIER ALAIN TAÏEB 《Pigment cell & melanoma research》1995,8(5):241-249
To study pigmentation, we have reconstructed an epidermis ex vivo with keratinocytes and melanocytes. Keratinocytes and melanocytes were grown first in primary cocultures and separately in secondary cultures, then seeded on a dead deepidermized dermis (Pruniéras type) at a 1:20 melanocyte/keratinocyte ratio. Reconstructed epidermis were grown in a special medium enriched with calcium and fetal bovine serum lifted for 15 days at the air-liquid interface. Using histology, immunohistochemistry and electron microscopy we have shown an excellent level of differentiation of the reconstructed epidermis and a physiologic distribution of dendritic melanocytes in the basal layer capable of melanosome transfer to keratinocytes. UVB irradiation 0.15 J/cm2× 5 consecutive days increased melanocyte numbers and stimulated pigmentation as evidenced macroscopically and microscopically and at the biochemical level. Following UVB irradiation melanosome transfer was markedly increased and isolated or clumps of melanosomes were seen in the basal layers as well as in the stratum corneum. This model allows the study of the physiology of pigmentation ex vivo. 相似文献