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661.
Among the cool season legume crops grown in India and the Indian sub-continent, peas are very popular and preferred by the
growers as well as consumers for various uses. The third largest area in pea cultivation is occupied by India after Canada
and Russia. Among the important and popular varieties of peas that are grown in India, several are from exotic background.
But very little work has been done to carry out the genetic diversity present in the widely adapted Indian pea varieties using
DNA markers. Twenty-four most popular and widely adapted varieties were subjected to RAPD analysis to find out the genetic
relatedness among them using 60 decamer primers. All the primers used in our study were found to be polymorphic and seven
of them showed 100% polymorphism. Out of 579 amplified products, 433 showed polymorphism (74.8%). On an average, 9.65 bands
were amplified per primer. Cluster analysis based on Jaccard’s similarity coefficient using UPGMA grouped all the tall type
varieties together, whereas, dwarf types formed two different clusters based upon their pedigree. The arithmetic mean heterozygosity
(H
av) value and marker index (MI) was found to be 0.496 and 4.787, respectively, thus this indicated the efficiency of RAPD as
a marker system. Moreover, the calculated value of probability of identical match by chance suggested that about 1053 genotypes can be unambiguously distinguish by employing 60 RAPD primers. 相似文献
662.
Choudhury Amir Sohail Choudhury Parthankar Gassah Rejoice 《Primates; journal of primatology》2022,63(2):185-186
663.
Cluster Computing - The internet is expanding its viewpoint into each conceivable part of the cutting-edge economy. Unshackled from our web programs today, the internet is characterizing our way of... 相似文献
664.
Farhana Khanam Md. Abu Sayeed Feroza Kaneez Choudhury Alaullah Sheikh Dilruba Ahmed Doli Goswami Md. Lokman Hossain Abdullah Brooks Stephen B. Calderwood Richelle C. Charles Alejandro Cravioto Edward T. Ryan Firdausi Qadri 《PLoS neglected tropical diseases》2015,9(4)
Background
Children bear a large burden of typhoid fever caused by Salmonella enterica serotype Typhi (S. Typhi) in endemic areas. However, immune responses and clinical findings in children are not well defined. Here, we describe clinical and immunological characteristics of young children with S. Typhi bacteremia, and antimicrobial susceptibility patterns of isolated strains.Methods
As a marker of recent infection, we have previously characterized antibody-in-lymphocyte secretion (TPTest) during acute typhoid fever in adults. We similarly assessed membrane preparation (MP) IgA responses in young children at clinical presentation, and then 7-10 days and 21-28 days later. We also assessed plasma IgA, IgG and IgM responses and T cell proliferation responses to MP at these time points. We compared responses in young children (1-5 years) with those seen in older children (6-17 years), adults (18-59 years), and age-matched healthy controls.Principal Findings
We found that, compared to age-matched controls patients in all age cohorts had significantly more MP-IgA responses in lymphocyte secretion at clinical presentation, and the values fell in all groups by late convalescence. Similarly, plasma IgA responses in patients were elevated at presentation compared to controls, with acute and convalescent IgA and IgG responses being highest in adults. T cell proliferative responses increased in all age cohorts by late convalescence. Clinical characteristics were similar in all age cohorts, although younger children were more likely to present with loss of appetite, less likely to complain of headache compared to older cohorts, and adults were more likely to have ingested antibiotics. Multi-drug resistant strains were present in approximately 15% of each age cohort, and 97% strains had resistance to nalidixic acid.Conclusions
This study demonstrates that S. Typhi bacteremia is associated with comparable clinical courses, immunologic responses in various age cohorts, including in young children, and that TPTest can be used as marker of recent typhoid fever, even in young children. 相似文献665.
Predicting the transmembrane regions is an important aspect of understanding the structures and architecture of different β-barrel membrane proteins. Despite significant efforts, currently available β-transmembrane region predictors are still limited in terms of prediction accuracy, especially in precision. Here, we describe PredβTM, a transmembrane region prediction algorithm for β-barrel proteins. Using amino acid pair frequency information in known β-transmembrane protein sequences, we have trained a support vector machine classifier to predict β-transmembrane segments. Position-specific amino acid preference data is incorporated in the final prediction. The predictor does not incorporate evolutionary profile information explicitly, but is based on sequence patterns generated implicitly by encoding the protein segments using amino acid adjacency matrix. With a benchmark set of 35 β-transmembrane proteins, PredβTM shows a sensitivity and precision of 83.71% and 72.98%, respectively. The segment overlap score is 82.19%. In comparison with other state-of-art methods, PredβTM provides a higher precision and segment overlap without compromising with sensitivity. Further, we applied PredβTM to analyze the β-barrel membrane proteins without defined transmembrane regions and the uncharacterized protein sequences in eight bacterial genomes and predict possible β-transmembrane proteins. PredβTM can be freely accessed on the web at http://transpred.ki.si/. 相似文献
666.
Ying-Hsia Chu Huihua Li Hui Shan Tan Valerie Koh Johnathan Lai Wai Min Phyo Yukti Choudhury Ravindran Kanesvaran Noan Minh Chau Chee Keong Toh Quan Sing Ng Puay Hoon Tan Balram Chowbay Min-Han Tan 《PloS one》2015,10(8)
Sunitinib is a tyrosine kinase inhibitor used as first-line treatment for metastatic renal cell carcinoma (mRCC). Asian ethnicity has been previously associated with lower clearance and greater toxicities for sunitinib treatment, relative to Caucasian ethnicity. Research focusing on identifying corresponding biomarkers of efficacy and toxicity has been hitherto conducted in Caucasian populations, and few of the reported associations have been externally validated. Our work thus aims to investigate candidate biomarkers in Asian patients receiving sunitinib, comparing the observed genotype effects with those reported in Caucasian populations. Using data from 97 Asian mRCC patients treated with sunitinib, we correlated 7 polymorphisms in FLT3, ABCB1, VEGFR2, ABCG2 and BIM with patient toxicities, response, and survival. We observed a stronger association of FLT3 738T genotype with leucopenia in our Asian dataset than that previously reported in Caucasian mRCC patients (odds ratio [OR]=8.0; P=0.03). We observed significant associations of FLT3 738T (OR=2.7), ABCB1 1236T (OR=0.3), ABCB1 3435T (OR=0.1), ABCB1 2677T (OR=0.4), ABCG2 421A (OR=0.3) alleles and ABCB1 3435, 1236, 2677 TTT haplotype (OR=0.1) on neutropenia. Primary resistance (OR=0.1, P=0.004) and inferior survival (progression-free: hazard ratio [HR]=5.5, P=0.001; overall: HR=5.0, P=0.005) were associated with the ABCB1 3435, 1236, 2677 TTT haplotype. In conclusion, ABCB1 and FLT3 polymorphisms may be helpful in predicting sunitinib toxicities, response and survival benefit in Asian mRCC patients. We have also validated the association between FLT3 738T and sunitinib-induced leucopenia previously reported in Caucasian populations, but have not validated other reported genetic associations. 相似文献
667.
The chlorophyll (Chl) fluorescence emission as well as excitation and polarization characteristics of chloroplasts from intact
cotyledons were determined in pumpkin seedlings after removal of one cotyledon (co-cotyledon) or apical bud or primary root,
or after kinetin treatment of derooted seedlings. Qualitatively, the fluorescence emission and excitation spectra of chloroplasts
were similar. The fluorescence emission spectra showed a maximum at 685 (F685) and a hump at 735 nm (F735), whereas the excitation
spectra showed peaks at 439, 471, 485, and 676 nm. The fluorescence intensities at F685 and F735 differed in various groups
of seedlings, as indicated by changes in their ratios. Similarly, the ratios of 471/439, 485/439, and 676/439 nm were also
different. Variability in the Chl fluorescence intensity values and the fluorescence polarization of chloroplasts prepared
from various seedling types may suggest a different degree of binding between the pigment complexes and light-harvesting Chl-protein
(LHCP), resulting in different rates of photoexcitation energy loss in the form of fluorescence emission. Kinetin treatment
improved the coupling of pigment complexes with reaction centre, as indicated by low polarization values in derooted and kinetin-treated
seedlings, which suggests the development of a suntype chloroplast.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
668.
669.
G. Péarez-Ponce de Leóan L. Garcíaa-Prieto V. Leóan-Réggagnon A. Choudhury 《Journal of fish biology》2000,57(2):303-325
In Lake Pátzcuaro in the Mesa Central of México, a total of 19 species of helminths was found in 598 fishes and comprised five digeneans, two monogeneans, four cestodes, one acanthocephalan and seven nematodes, of which ten species were represented by larval or immature states. The richest and most diverse helminth communities were found in the native carnivorous goodeid Alloophorus robustus. In general, the helminth communities in the different fish species were not particularly species rich and the parasite assemblages were numerically dominated by larvae of the bird trematode, Posthodiplostomum minimum. Patterns of helminth community richness and diversity were similar to those previously observed in north-temperate freshwater fishes. Most enteric helminths occurred with low abundance and only a small proportion of the gut helminth communities was numerically dominated by any one species. Helminths dominating their enteric communities showed some level of host specificity. Helminth communities in carnivorous fish species were generally richer than those in herbivores and detritivores, with the exception of the predominantly herbivorous Goodea atripinnis. The helminth fauna of introduced fishes, Cyprinus carpio, Micropterus salmoides and Oreochromis niloticus , consisted of either few or no host-specific adult helminth(s) translocated from their original geographical areas and by larval stages of helminths of piscivorous birds. Based on the geological history of the area and the biogeography of the endemic fish fauna, it is hypothesized that host-switching and relationships with the nearctic fauna have been fundamental in determining the helminth fauna of the endemic fish hosts. 相似文献
670.