全文获取类型
收费全文 | 2284篇 |
免费 | 206篇 |
国内免费 | 2篇 |
专业分类
2492篇 |
出版年
2023年 | 7篇 |
2022年 | 15篇 |
2021年 | 30篇 |
2020年 | 24篇 |
2019年 | 17篇 |
2018年 | 39篇 |
2017年 | 34篇 |
2016年 | 60篇 |
2015年 | 109篇 |
2014年 | 128篇 |
2013年 | 130篇 |
2012年 | 175篇 |
2011年 | 154篇 |
2010年 | 104篇 |
2009年 | 102篇 |
2008年 | 142篇 |
2007年 | 150篇 |
2006年 | 172篇 |
2005年 | 144篇 |
2004年 | 114篇 |
2003年 | 123篇 |
2002年 | 136篇 |
2001年 | 23篇 |
2000年 | 18篇 |
1999年 | 22篇 |
1998年 | 33篇 |
1997年 | 20篇 |
1996年 | 29篇 |
1995年 | 23篇 |
1994年 | 31篇 |
1993年 | 18篇 |
1992年 | 25篇 |
1991年 | 14篇 |
1990年 | 11篇 |
1989年 | 17篇 |
1988年 | 12篇 |
1987年 | 7篇 |
1986年 | 6篇 |
1985年 | 13篇 |
1983年 | 6篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 7篇 |
1978年 | 4篇 |
1976年 | 9篇 |
1975年 | 3篇 |
1972年 | 3篇 |
1971年 | 3篇 |
1965年 | 2篇 |
1951年 | 2篇 |
排序方式: 共有2492条查询结果,搜索用时 0 毫秒
191.
192.
Monika Martiniaková Radoslav Omelka Birgit Grosskopf Alena Jančová 《Acta veterinaria Scandinavica》2010,52(1):58
Background
Free-living wild rodents are often used as zoomonitors of environmental contamination. In the present study, accumulation of cadmium (Cd), copper (Cu), iron (Fe), and zinc (Zn) in critical organs of yellow-necked mice (Apodemus flavicollis) and bank voles (Myodes glareolus) trapped in a polluted area in Nováky, Slovakia was investigated. 相似文献193.
Stable haploid poplar callus lines from immature pollen culture 总被引:3,自引:0,他引:3
Embryogenesis and plant regeneration have been obtained from isolated immature pollen of two poplar hybrids ( Populus nigra L. × hybrid 'Aue1' and 'Aue2'). In total, 1487 calli or embryos, respectively, larger than 1 mm were generated in a 2-year study. By using a cytokinin containing induction medium, on average 19 calli per responsive immature catkin were formed. Additional supplementation with auxin in 2002 increased the frequency to 72 calli per catkin. Microsatellite marker analyses confirmed haploid origin in most regenerants studied. So far six out of eight obtained regenerative callus lines have maintained their haploid level up to 24 months of development. A number of haploid and doubled haploid plants of different lines are available and have been transferred to soil. 相似文献
194.
Plasma distribution of apoA-IV in patients with coronary artery disease and healthy controls 总被引:2,自引:0,他引:2
Ezeh B Haiman M Alber HF Kunz B Paulweber B Lingenhel A Kraft HG Weidinger F Pachinger O Dieplinger H Kronenberg F 《Journal of lipid research》2003,44(8):1523-1529
Recent studies showed lower apolipoprotein A-IV (apoA-IV) plasma concentrations in patients with coronary artery disease (CAD). The actual distribution of the antiatherogenic apoA-IV in human plasma, however, is discussed controversially and it was never investigated in CAD patients. We therefore developed a gentle technique to separate the various apoA-IV-containing plasma fractions. Using a combination of precipitation of all lipoproteins with 40% phosphotungstic acid and 4 M MgCl2, as well as immunoprecipitation of all apoA-I-containing particles with an anti-apoA-I antibody, we obtained three fractions of apoA-IV: lipid-free apoA-IV (about 4% of total apoA-IV), apoA-IV associated with apoA-I (LpA-I:A-IV, 12%), and apoA-I-unbound but lipoprotein-containing apoA-IV (LpA-IV, 84%). We compared these three apoA-IV fractions between 52 patients with a history of CAD and 52 age- and sex-matched healthy controls. Patients had significantly lower apoA-IV levels when compared to controls (10.28 +/- 3.67 mg/dl vs. 11.85 +/- 2.82 mg/dl, P = 0.029), but no major differences for the three plasma apoA-IV fractions. We conclude that our gentle separation method reveals a different distribution of apoA-IV than in many earlier studies. No major differences exist in the apoA-IV plasma distribution pattern between CAD patients and controls. Therefore, the antiatherogenic effect of apoA-IV has to be explained by other functional properties of apoA-IV (e.g., the antioxidative characteristics). 相似文献
195.
Effect of hemodialysis on the antioxidative properties of serum 总被引:3,自引:0,他引:3
Mayer B Zitta S Greilberger J Holzer H Reibnegger G Hermetter A Oettl K 《Biochimica et biophysica acta》2003,1638(3):267-272
In patients with chronic renal failure undergoing regular hemodialysis (HD), oxidative stress is involved in the development of dialysis-related pathologies. The aim of the study was to measure the effect of HD treatment on the general antioxidative status of serum with special consideration of the specific oxidizability of lipids and proteins. Indicators for the oxidative/antioxidative status of plasma were monitored at the beginning and at the end of a dialysis session on the arterial and venous side of the dialyzer. A decrease in the antioxidant status was accompanied by an increased oxidizability of proteins as well as lipids during HD treatment. During the first passage of the dialyzer, the lag time of lipid oxidation decreased from 114.0+/-19.8 to 81.5+/-18.9 min, the lag time of protein oxidation decreased from 105.0+/-24.6 to 72.9+/-21.3 min and the total antioxidative status decreased from 518+/-24 to 252+/-124 microM trolox equivalents. The carbonyl content of serum proteins was high in patients with end stage renal disease (ESRD) (3.9+/-1.1 vs. 0.9+/-0.1 nmol/mg in controls) but did not change significantly during dialysis procedure. Our data demonstrate that the susceptibility of serum lipids and proteins to oxidative modification is severely increased by HD treatment. 相似文献
196.
Steinlechner S Jacobmeier B Scherbarth F Dernbach H Kruse F Albrecht U 《Journal of biological rhythms》2002,17(3):202-209
The Per1 and Per2 genes are components of the mammalian circadian clock. Mutations in these genes alter phase resetting in response to a nocturnal light pulse, and Per2 mutant mice are known to become arrhythmic in constant darkness. We show that under constant light conditions, Per2 mutant mice exhibit robust activity rhythms as well as body temperature rhythms with a period length that is less than 24 h. In Per1 mutants, the period length of both activity and body temperature rhythms is longer than 24 h in constant light. Per1 mutants prolong their period length (tao) when illuminance is increased, whereas Per2 mutants shorten their endogenous period. Additionally, the authors show that the circadian pattern of Per1 and Per2 gene expression in mice is modified under different photoperiods and that there is a mutual influence of these genes on their timing of expression. We propose that, in mice, the phase relationship between Per1 and Per2 gene expression might be critical for transducing day length information to the organism. Per1 could be part of a morning oscillator tracking dawn, and Per2 could be part of an evening oscillator tracking dusk. 相似文献
197.
Stable isotope and trace element geochemistry of Upper Cretaceous carbonates and belemnite rostra (Middle Campanian, north Germany) 总被引:1,自引:0,他引:1
Trace element contents and stable isotopic composition of Middle Campanian marl-limestone rhythmites and belemnite rostra of Belemnitella mucronata were investigated. High strontium and low iron as well as manganese and magnesium contents of belemnite calcite and bulk rock samples suggest no diagenetic overprint. However, the orange-coloured cathodoluminescence of coccolith-rich sediments indicates diagenetic cementation and/or recrystallization. The non-luminescent belemnite rostra reveal an extraordinary preservation of the microstructures that is interpreted to have been favoured by a silification of the outer rim of the belemnite rostra. Carbon isotope ratios of the coccolith limestones and belemnite rostra are comparable, with higher δ13C variations observed for belemnite calcite. The 1.5-2‰ depletion in δ18O of the marl-limestone rhythmites relative to belemnite calcite is explained by diagenetic alteration of the sediments. Palaeotemperatures, calculated from the δ18O values of the well-preserved belemnite rostra, are around 12.5 ± 2 °C and suggest rather low sea-surface temperatures for the Middle Campanian epicontinental sea of north Germany assuming a water depth of less than 100 m. 相似文献
198.
Long-distance bird migration consists of a series of stopovers (for refuelling) and flights, with flights taking little time compared to stopovers. Therefore, it has been hypothesized that birds minimize the total time taken for migration through efficient stopover behaviour. Current optimality models for stopover include (1) the fixed expectation rule and (2) the global update rule. These rules maximize the speed of migration by determining the optimal departure fuel load for a given fuel deposition rate. We were interested in simple behavioural rules approaching the stopover behaviour of real birds and how these rules compare to the time minimizing models above with respect to the total time taken for migration. The simple strategies were to stay at a site (1) until a fixed fuel load was reached or (2) for a constant number of days. We simulated migration of small nocturnal passerine birds across an environment of continuously distributed but variable fuel deposition rates, and investigated the influence of different stopover strategies on the duration of migration. Staying for a constant number of days at each stopover site, irrespective of the fuel deposition rate, resulted in only slightly longer than minimum values for migration duration. Additionally, the constant stopover duration, e.g. 10 days, may change by a day or two (per stopover) without having a large effect on total migration duration. There is therefore a possibility that real birds may be close to optimal migration speed without the need for very complex behaviour. When assessing the sensitivity of migration duration to factors other than stopover duration, we found that flight costs, search and settling time, mean fuel deposition rate and the accuracy in the choice of flight direction were the factors with the largest influence. Our results suggest that migrating birds can approximate optimal stopover duration relatively easy with a simple rule, and that other factors, e.g. those above, are more relevant for travel time. 相似文献
199.
200.
Peroxiredoxins 总被引:7,自引:0,他引:7
Present knowledge on peroxiredoxins is reviewed with special emphasis on catalytic principles, specificities and biological function. Peroxiredoxins are low efficiency peroxidases using thiols as reductants. They appear to be fairly promiscuous with respect to the hydroperoxide substrate; the specificities for the donor substrate vary considerably between the subfamilies, comprising GSH, thioredoxin, tryparedoxin and the analogous CXXC motifs in bacterial AhpF proteins. Peroxiredoxins are definitely responsible for antioxidant defense in bacteria (AhpC), yeast (thioredoxin peroxidase) and trypanosomatids (tryparedoxin peroxidase). They are considered to determine virulence of mycobacteria and trypanosomatids. In higher plants they are involved in balancing hydroperoxide production during photosynthesis. In higher animals peroxiredoxins appear to be involved in the redox-regulation of cellular signaling and differentiation, displaying in part opposite effects. 相似文献