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11.
Over-expression of superoxide dismutase and lack of surface-thiols in spermatozoa: inherent defects in oligospermia. 总被引:1,自引:0,他引:1
S Sinha K G Pradeep M Laloraya D Warikoo 《Biochemical and biophysical research communications》1991,174(2):510-517
The infertility associated with oligospermia had been suspected to be a direct consequence of the impairment in fertilizing ability of the spermatozoa rather than the presence of low sperm number per se. This study defines some of these impairments, which include an over expression of superoxide dismutase, poor superoxide anion radical generation capacity and a lack of sperm-surface thiols in oligospermia. The abnormal biochemical make-up of spermatozoa could explain their low fertilizing ability in case of oligospermia. 相似文献
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Vibrio el tor cells contain a constitutive reductase enzyme which converts nitrofurantoin to an active principle that is responsible for the observed antibacterial activity of the drug. Acquisition of resistance of this strain towards nitrofurantoin is associated with the loss of this reductase. This enzyme is located in the periplasmic region of the nitrofurantoin-sensitive cells, and seems to play an important role in transporting the drug into the cells. 相似文献
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Summary A main yolk component in the oocytes of the pulmonate snailPlanorbarius corneus L. has been isolated and identified as the iron storage protein ferritin by its ultrastructure, iron content, immuunological properties and behaviour in disc electrophoresis. As judged from acrylamide electrophoresis data and ultrastructural observations, yolk ferritin is an exogenous protein which is synthesised in the hepatopancreas and taken up by the oocytes by endocytosis. 相似文献
15.
Summary Magnitude of Na and Cl accumulation around wheat roots was studied under different transpiration conditions in a loamy sand soil salinized with sodium chloride to an electrical conductivity of 4.1 mmho/cm in the saturation extract. A significant correlation was observed between rate of water loss per unit root length and Na and Cl content of the soil closely adhering to the roots. Under high transpiration condition, maximum ion accumulation occurred in the apparent free space of roots followed by the soil closely adhering to the roots. Results indicate that salt concentration at the root surface is markedly altered under the influence of transpiration rate such that under high transpiration conditions, the plant roots may be exposed to a much higher salt concentration than that anticipated from an analysis of the bulk soil samples.Department of Soil and Water EngineeringDepartment of Soils 相似文献
16.
Sodium butyrate produces reversible changes in morphology, growth rate, and enzyme activities of several mammalian cell types in culture. Some of these changes are similar to those produced by agents which increase the intracellular level of adenosine 3',5'-cyclic monophosphate (cAMP) or by analogs of cAMP. Sodium butyrate increases the intracellular level of cAMP by about two fold in neuroblastoma cells; therefore, some of the effects of sodium butyrate on these cells may in part be mediated by cAMP. Sodium butyrate appears to have properties of a good chemotherapeutic agent for neuroblastoma tumors because the treatment of neuroblastoma cells in culture causes cell death and "differentiation"; however, it is either innocuous or produces reversible morphological and biochemical alterations in other cell types. 相似文献
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The differential reactivities of individual lysines on porcine testicular calmodulin were determined by trace labeling with high specific activity [3H]acetic anhydride as a function of the molar ratio of Ca2+ to calmodulin. In progressing from the Ca2+-depleted form of the protein to a Ca2+:calmodulin molar ratio of 5:1, six of the seven lysyl residues exhibited a modest 1.5- to 3.0-fold increase in reactivity. Lys 75, in contrast, was enhanced in reactivity greater than 20-fold. When the change in reactivity of each lysine was normalized as a percentage of the maximum change, most of the residues were found to fall into two distinct classes. One class, comprising lysines 94 and 148 from the two carboxy terminal Ca2+-binding domains 3 and 4, respectively, exhibited about 90% of their reactivity change when the Ca2+:calmodulin molar ratio was 2:1, and these residues were perturbed very little upon further addition of Ca2+. The other class, encompassing lysines 13, 21, and 30 from the amino terminal domain 1 and Lys 75 from the extended helix connecting the two globular lobes of calmodulin, underwent most of their overall reactivity change (55-70%) between 2 and 5 equivalents of Ca2+ per mol of calmodulin. Lys 77 was distinct in its pattern of change, undergoing approximately equal changes with each Ca2+ increment. These results are consistent with a model where Ca2+ first binds to the two carboxy terminal sites of calmodulin with no apparent preference, concomitant with minor alterations in the microenvironments of lysines in the unoccupied amino terminal domains. The third and fourth Ca2+ ions then bind to these latter two domains, again with no evidence of preference, with little change in the lysine reactivities at the carboxy terminus of the molecule. The environments of groups in the central helix appear to undergo changes in a manner that reflects their proximity to the amino and carboxy terminal domains. In the course of this work, it was found that Lys 94 in apocalmodulin is specifically perturbed by the addition of EGTA, suggesting that the chelating agent may interact with calmodulin at or near the third Ca2+-binding domain. 相似文献
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