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941.
华南寒武系地层中广泛富集微体骨骼化石,为解决某些疑难化石的亲缘关系及研究早期后生动物的演化提供了重要化石证据。在贵州剑河八郎"清虚洞组"中发现一些管状微体骨骼化石。经鉴定后主要有4属,分别为小钻孔螺Torellella、似软舌螺Hyolithellus、鞘状螺Coleoloides和表面具鳞片状管状化石Mongolitubulus squamifer。Mongolitubulus分布范围较为广泛,本文结合前人对该化石亲缘关系的探讨及剑河寒武系"清虚洞组"化石的特征,推测M.squamifer可能是高肌虫的装饰刺。 相似文献
942.
Jacob C. Ulirsch Jeffrey M. Verboon Shideh Kazerounian Michael H. Guo Daniel Yuan Leif S. Ludwig Robert E. Handsaker Nour J. Abdulhay Claudia Fiorini Giulio Genovese Elaine T. Lim Aaron Cheng Beryl B. Cummings Katherine R. Chao Alan H. Beggs Casie A. Genetti Colin A. Sieff Peter E. Newburger Hanna T. Gazda 《American journal of human genetics》2019,104(2):356
943.
Hydrogenases are metalloproteins capable of catalyzing the interconversion between molecular hydrogen and protons and electrons. The iron–sulfur clusters within the enzyme enable rapid relay of electrons which are either consumed or generated at the active site. Their unparalleled catalytic efficiency has attracted attention, especially for potential use in H2 production and/or fuel cell technologies. However, there are limitations to using hydrogenases, especially due to their high O2 sensitivity. The subclass, called [FeFe] hydrogenases, are particularly more vulnerable to O2 but proficient in H2 production. In this review, we provide an overview of mechanistic and protein engineering studies focused on understanding and enhancing O2 tolerance of the enzyme. The emphasis is on ongoing studies that attempt to overcome O2 sensitivity of the enzyme while it catalyzes H2 production in an aerobic environment. We also discuss pioneering attempts to utilize the enzyme in biological H2 production and other industrial processes, as well as our own perspective on future applications. 相似文献
944.
Estrogen inhibits osteoclasts formation and bone resorption via microRNA-27a targeting PPARγ and APC
Lei Guo Kaizhe Chen Jun Yuan Ping Huang Xing Xu Changwei Li Niandong Qian Jin Qi Zhiliang Shao Lianfu Deng Chuan He Jiping Xu 《Journal of cellular physiology》2019,234(1):581-594
Inhibition of osteoclasts formation and bone resorption by estrogen is very important in the etiology of postmenopausal osteoporosis. The mechanisms of this process are still not fully understood. Recent studies implicated an important role of microRNAs in estrogen-mediated responses in various cellular processes, including cell differentiation and proliferation. Thus, we hypothesized that these regulatory molecules might be implicated in the process of estrogen-decreased osteoclasts formation and bone resorption. Western blot, quantitative real-time polymerase chain reaction, tartrate-resistant acid phosphatase staining, pit formation assay and luciferase assay were used to investigate the role of microRNAs in estrogen-inhibited osteoclast differentiation and bone resorption. We found that estrogen could directly suppress receptor activator of nuclear factor B ligand/macrophage colony-stimulating factor-induced differentiation of bone marrow-derived macrophages into osteoclasts in the absence of stromal cell. MicroRNA-27a was significantly increased during the process of estrogen-decreased osteoclast differentiation. Overexpressing of microRNA-27a remarkably enhanced the inhibitory effect of estrogen on osteoclast differentiation and bone resorption, whereas which were alleviated by microRNA-27a depletion. Mechanistic studies showed that microRNA-27a inhibited peroxisome proliferator-activated receptor gamma (PPARγ) and adenomatous polyposis coli (APC) expression in osteoclasts through a microRNA-27a binding site within the 3′-untranslational region of PPARγ and APC. PPARγ and APC respectively contributed to microRNA-27a-decreased osteoclast differentiation and bone resorption. Taken together, these results showed that microRNA-27a may play a significant role in the process of estrogen-inhibited osteoclast differentiation and function. 相似文献
945.
Luoyang Li Xingliang Zhang Christian B. Skovsted Hao Yun Bing Pan Guoxiang Li 《Palaeontology》2019,62(4):515-532
Hyoliths were among the earliest biomineralizing metazoans in Palaeozoic marine environments. They have been known for two centuries and widely assigned to lophotrochozoans. However, their origin and relationships with modern lophotrochozoan clades have been a longstanding palaeontological controversy. Here, we provide broad microstructural data from hyolith conchs and opercula from the lower Cambrian Xinji Formation of North China, including two hyolithid genera and four orthothecid genera as well as unidentified opercula. Results show that most hyolith conchs contain a distinct aragonitic lamellar layer that is composed of foliated aragonite, except in the orthothecid New taxon 1 that has a crossed foliated lamellar microstructure. Opercula are mostly composed of foliated aragonite and occasionally foliated calcite. These blade or lath‐like microstructural fabrics coincide well with biomineralization of Cambrian molluscs rather than lophophorates, as exemplified by the Cambrian members of the tommotiid‐brachiopod linage. Accordingly, we propose that hyoliths and molluscs might have inherited their biomineralized skeletons from a non‐mineralized or weakly mineralized common ancestor rather than as a result of convergence. Consequently, from the view of biomineralization, the homologous shell microstructures in Cambrian hyoliths and molluscs strongly strengthen the phylogenetic links between the two groups. 相似文献
946.
Yu‐Qi Xu Hong Wang Rong‐Li Qin Li‐Jing Fang Zhuang Liu Shuo‐Shuo Yuan Ying‐Ping Gai Xian‐Ling Ji 《Physiologia plantarum》2019,167(3):302-316
The quality and quantity of mulberry leaves are often affected by various environmental factors. The plant NPR1 and its homologous genes are important for plant systemic acquired resistance. Here, the full‐length cDNAs encoding the NPR1 and NPR4 genes (designated MuNPR1 and MuNPR4, respectively) were isolated from Morus multicaulis. Sequence analysis of the amino acids and protein modeling of the MuNPR1 and MuNPR4 proteins showed that MuNPR1 shares some conserved characteristics with its homolog MuNPR4. MuNPR1 was shown to have different expression patterns than MuNPR4 in mulberry plants. Interestingly, MuNPR1 or MuNPR4 transgenic Arabidopsis produced an early flowering phenotype, and the expression of the pathogenesis‐related 1a gene was promoted in MuNPR1 transgenic Arabidopsis. The MuNPR1 transgenic plants showed more resistance to Pseudomonas syringae pv. tomato DC3000 (Pst. DC3000) than did the wild‐type Arabidopsis. Moreover, the ectopic expression of MuNPR1 might lead to enhanced scavenging ability and suppress collase accumulation. In contrast, the MuNPR4 transgenic Arabidopsis were hypersensitive to Pst. DC3000 infection. In addition, transgenic Arabidopsis with the ectopic expression of either MuNPR1 or MuNPR4 showed sensitivity to salt and drought stresses. Our data suggest that both the MuNPR1 and MuNPR4 genes play a role in the coordination between signaling pathways, and the information provided here enables the in‐depth functional analysis of the MuNPR1 and MuNPR4 genes and may promote mulberry resistance breeding in the future. 相似文献
947.
Gang Qun Chang Scott X. Lin Guigang Zhao Qiong Mao Bing Zeng De-Hui 《Plant and Soil》2019,436(1-2):109-122
Plant and Soil - Nitrogen (N) addition could affect litter decomposition through its direct effects on soil N availability and indirect effects on initial litter chemistry. The aim of this study... 相似文献
948.
Christine R. Beck Claudia M.B. Carvalho Zeynep C. Akdemir Fritz J. Sedlazeck Xiaofei Song Qingchang Meng Jianhong Hu Harsha Doddapaneni Zechen Chong Edward S. Chen Philip C. Thornton Pengfei Liu Bo Yuan Marjorie Withers Shalini N. Jhangiani Divya Kalra Kimberly Walker Adam C. English James R. Lupski 《Cell》2019,176(6):1310-1324.e10
949.
Yuan Wang Ren‐Jie Tang Xiyan Yang Xiaojiang Zheng Qiaolin Shao Qing‐Lin Tang Aigen Fu Sheng Luan 《Plant, cell & environment》2019,42(2):673-687
Multiple transporters and channels mediate cation transport across the plasma membrane and tonoplast to regulate ionic homeostasis in plant cells. However, much less is known about the molecular function of transporters that facilitate cation transport in other organelles such as Golgi. We report here that Arabidopsis KEA4, KEA5, and KEA6, members of cation/proton antiporters‐2 (CPA2) superfamily were colocalized with the known Golgi marker, SYP32‐mCherry. Although single kea4,5,6 mutants showed similar phenotype as the wild type under various conditions, kea4/5/6 triple mutants showed hypersensitivity to low pH, high K+, and high Na+ and displayed growth defects in darkness, suggesting that these three KEA‐type transporters function redundantly in controlling etiolated seedling growth and ion homeostasis. Detailed analysis indicated that the kea4/5/6 triple mutant exhibited cell wall biosynthesis defect during the rapid etiolated seedling growth and under high K+/Na+ condition. The cell wall‐derived pectin homogalacturonan (GalA)3 partially suppressed the growth defects and ionic toxicity in the kea4/5/6 triple mutants when grown in the dark but not in the light conditions. Together, these data support the hypothesis that the Golgi‐localized KEAs play key roles in the maintenance of ionic and pH homeostasis, thereby facilitating Golgi function in cell wall biosynthesis during rapid etiolated seedling growth and in coping with high K+/Na+ stress. 相似文献
950.
Zhaozhong Feng Xiangyang Yuan Silvano Fares Francesco Loreto Pin Li Yasutomo Hoshika Elena Paoletti 《Plant, cell & environment》2019,42(6):1939-1949
Isoprene and monoterpenes (MTs) are among the most abundant and reactive volatile organic compounds produced by plants (biogenic volatile organic compounds). We conducted a meta‐analysis to quantify the mean effect of environmental factors associated to climate change (warming, drought, elevated CO2, and O3) on the emission of isoprene and MTs. Results indicated that all single factors except warming inhibited isoprene emission. When subsets of data collected in experiments run under similar change of a given environmental factor were compared, isoprene and photosynthesis responded negatively to elevated O3 (?8% and ?10%, respectively) and drought (?15% and ?42%), and in opposite ways to elevated CO2 (?23% and +55%) and warming (+53% and ?23%, respectively). Effects on MTs emission were usually not significant, with the exceptions of a significant stimulation caused by warming (+39%) and by elevated O3 (limited to O3‐insensitive plants, and evergreen species with storage organs). Our results clearly highlight individual effects of environmental factors on isoprene and MT emissions, and an overall uncoupling between these secondary metabolites produced by the same methylerythritol 4‐phosphate pathway. Future results from manipulative experiments and long‐term observations may help untangling the interactive effects of these factors and filling gaps featured in the current meta‐analysis. 相似文献