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991.
0.2 W.m-2的UV-B辐射不仅能诱导整体蚕豆叶片气孔导度和开度的显著降低,而且能明显降低蚕豆叶肉光合活性,但该强度的UV-B辐射却不能明显影响离体表皮条的气孔开度.说明0.2W.m-2的UV-B主要通过间接途径调控了蚕豆叶片气孔运动.借助药理学试验和激光扫描共聚焦显微镜技术,进一步对该间接效应过程中是否有NO和H2O2的参与进行了探讨.结果显示:NO专一性清除剂cPT IO和一氧化氮合酶(NO S)抑制剂L-NAM E均能有效地抑制UV-B辐射诱导的叶片气孔关闭和保卫细胞内源NO水平的升高;H2O2清除剂抗坏血酸(A SC)和过氧化氢酶(CAT)也能有效地逆转UV-B辐射诱导的气孔关闭和保卫细胞内源H2O2含量的升高.另外,外源NO或H2O2处理也能有效地诱导叶片气孔关闭.结果说明0.2W.m-2的UV-B辐射对蚕豆叶片气孔关闭的间接诱导与NO和H2O2有关. 相似文献
992.
【目的】从代谢流量分配的角度,探讨Genome shuffling导致链霉菌ε-聚赖氨酸合成量提升的原因。【方法】从葡萄糖耐受型的亲本菌株Streptomyces sp.AS32和ε-聚赖氨酸耐受型的亲本菌株Streptomyces albulus F15出发,进行三轮Genome shuffling,筛选得到ε-聚赖氨酸产量提高的链霉菌株Streptomyces sp.AF3-44,采用通量分析方法构建链霉菌ε-聚赖氨酸合成代谢网络,并对上述3株菌的代谢通量进行比较。【结果】AF3-44的ε-聚赖氨酸摇瓶产量为3.1 g/L,较AS32和F15分别提高了34%和29%。3株菌株中AS32三羧酸循环(TCA)的代谢通量最高;F15磷酸戊糖途径(PPP)代谢通量最高;AF3-44流向赖氨酸合成前体天冬氨酸以及ε-聚赖氨酸的通量最高,TCA和PPP通量位于两亲本菌株的中间水平,其中TCA中流向异柠檬酸的通量分别为AS32和F15的77%和116%,PPP中流向5-磷酸核酮糖的通量分别为AS32和F15的149%和92%。【结论】Genome shuffling导致了代谢流的重新分布,流向前体赖氨酸和ε-聚赖氨酸通量的增加,以及PPP和TCA通量配比的改变是链霉菌ε-聚赖氨酸合成量增加的重要因素。 相似文献
993.
推测人骨形成蛋白3羧基端的127氨基酸的肽段为其成熟肽(hBMP3m)。将编码此成熟肽的cDNA插入含PL启动子的表达质粒pDH中,构建表达质粒pDHB3m,转化大肠杆菌DH5α。42℃热诱导6h后表达量达到最高水平,约占菌体总蛋白28%;表达产物以包涵体形式存在。包涵体经分离和洗涤后,溶解于尿素,在变性溶解状态下经阳离子交换层析,目的蛋白纯度至少在95%以上。再经稀释复性。然后将纯化、复性的重组人骨形成蛋白3成熟肽(rhBMP3m)植入小鼠肌肉间隙,于不同时间取材观察,在局部可见典型的软骨形成、软骨内成骨以及骨组织形成的过程,证实所制备的rhBMP3m具有明显的异位诱骨活性。 相似文献
994.
Overexpression of Jatropha curcas Defensin (JcDef) Enhances Sheath Blight Disease Resistance in Tobacco 下载免费PDF全文
Qingwei Wang Shuhua Zhu Yao Liu Rui Li Shancai Tan Shenghua Wang Lin Tang Fang Chen 《Journal of Phytopathology》2017,165(1):15-21
Plant defensins are small, basic, cysteine‐rich peptides, belonging to the antimicrobial peptide superfamily, commonly found in the plant kingdom. In this study, we cloned and characterized a plant defensin gene from Jatropha curcas (JcDef). JcDef carried conserved receptor binding sites and a cysteine motif, and it was phylogenetically grouped together with defensin Ec‐AMP‐D2‐like in Elaeis guineensis. JcDef is localized to cytoplasm and highly expressed in young tissues with fast metabolism such as cotyledons and stem apexes. Transgenic expression of JcDef in tobacco showed enhanced resistance against sheath blight disease caused by R. solani, indicating the antibacterial function. 相似文献
995.
Yuze Shang Hansen Wang Valentina Mercaldo Xiangyao Li Tao Chen Min Zhuo 《Journal of neurochemistry》2009,111(3):635-646
Fragile X syndrome (FXS), a common form of inherited mental retardation, is caused by the lack of fragile X mental retardation protein (FMRP). The animal model of FXS, Fmr1 knockout mice, have deficits in the Morris water maze and trace fear memory tests, showing impairment in hippocampus-dependent learning and memory. However, results for synaptic long-term potentiation (LTP), a key cellular model for learning and memory, remain inconclusive in the hippocampus of Fmr1 knockout mice. Here, we demonstrate that FMRP is required for glycine induced LTP (Gly-LTP) in the CA1 of hippocampus. This form of LTP requires activation of post-synaptic NMDA receptors and metabotropic glutamateric receptors, as well as the subsequent activation of extracellular signal-regulated kinase (ERK) 1/2. However, paired-pulse facilitation was not affected by glycine treatment. Genetic deletion of FMRP interrupted the phosphorylation of ERK1/2, suggesting the possible role of FMRP in the regulation of the activity of ERK1/2. Our study provide strong evidences that FMRP participates in Gly-LTP in the hippocampus by regulating the phosphorylation of ERK1/2, and that improper regulation of these signaling pathways may contribute to the learning and memory deficits observed in FXS. 相似文献
996.
Plant regeneration through somatic embryogenesis of Areca catechu L. was established using leaf, root and stem segments as explants. Embryogenic callus was induced and maintained on medium
supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 3,6-dichloro-2-methoxybenzoic acid (dicamba) at concentrations
2, 4, 6 and 8 mg dm−3 in darkness. Somatic embryos were found on primary callus in the presence of 2 and 4 mg dm−3 dicamba and during subculture on 2 – 8 mg dm−3 2,4-D or 2 – 4 mg dm−3 dicamba-containing media. Plantlet conversion from embryos was successfully achieved on growth regulator-free medium. The
plants grew well when transplanted to containers in shaded greenhouse. 相似文献
997.
水稻叶片在自然衰老过程中1,5-二磷酸核酮糖羧化酶/加氧酶的降解(英文) 总被引:1,自引:0,他引:1
1,5二磷酸核酮糖羧化酶/加氧酶(Rubisco)是光合碳同化的关键酶,研究其降解机理对合理调控水稻生长后期光合衰退具有重要意义。前人用人为诱导植物衰老的方法,研究了Rubisco的降解机理,认为该酶降解之前,必需发生亚基间的交联聚合和向类囊体膜转移,这样在结构和空间上有利于水解酶的作用。我们用自然衰老叶片进行研究的结果表明:Rubisco在降解过程中其比活基本保持恒定,意味着未发生酶的失活,也就是说酶结构未发生根本性改变,由此也可初步判断酶未发生亚基间的交联聚合(已证明亚基交联可导致酶失活)。接着用SDSPAGE和蛋白印迹技术证实了上述观点:Rubisco降解之前只有极少量的大亚基聚合体,随后同未聚合大亚基一起很快降解。此外,研究结果进一步表明酶分子在降解之前有少量与叶绿体膜结合,但降解过程中并未见膜结合蛋白增加。根据上述结果我们认为,亚基间交联聚合和向膜转移并非水稻叶片自然衰老时Rubisco降解的必要条件。 相似文献
998.
Wei Zhang Hiroshi Kudo Shiho Fujisaka Toshiro Sugiyama Naihong Chen Terumi Takahara 《Biochemical and biophysical research communications》2010,391(4):1731-1736
Non-alcoholic steatohepatitis (NASH) develops in a subset of patients with non-alcoholic fatty liver disease (NAFLD), but the exact mechanisms involved in the progression of NAFLD to NASH remain poorly understood. We investigated the role of tumor necrosis factor-α (TNF-α) in the apoptosis of hepatocytes that is related to the severity of NASH. We separated primary hepatocytes from the NAFLD liver caused by a high-fat diet. The production of intracellular reactive oxygen species was increased in steatotic hepatocytes, which were also sensitive to TNF-α. This factor induced significant apoptosis through the signal-regulating kinase 1 (ASK1) and c-Jun N-terminal kinase (JNK) pathway. We describe here a novel culture model of steatotic hepatocytes separated from the NAFLD liver, and demonstrate that TNF-α induces their apoptosis in vitro. 相似文献
999.
Shengsong Xu Rui Guo Pei-Zhi Li Ke Li Yu Yan Jianlong Chen Guang Wang Beate Brand-Saberi Xuesong Yang Xin Cheng 《Journal of cellular physiology》2019,234(9):15167-15181
Dexamethasone (Dex), a synthetic glucocorticoid (GC) with long-lasting treatment effects, has been proved to exert a modulatory effect on osteoblast proliferation and differentiation during embryonic osteogenesis. However, it is still controversial if Dex exposure influences endochondral ossification and the underlying mechanism. In this study, chick embryos in vivo and preosteoblast cell cultures in vitro were utilized to investigate the effects of Dex on osteoblast formation and differentiation during the skeletal development. We first demonstrated that Dex exposure could shorten the long bones of 17-day chick embryos in vivo, and also downregulated the expressions of osteogenesis-related genes. Next, we established that Dex exposure inhibited the proliferation and viability of preosteoblasts-MC3TC-E1 cells, and the addition of insulin-like growth factor 1 (IGF-1) could dramatically rescue these negative effects. On the basis of remarkable changes in the rescue experiments, we next verified the important role of angiogenesis in osteogenesis by culturing isolated embryonic phalanges in Dulbecco's modified Eagle's medium culture or on the chick chorioallantoic membrane (CAM). Then, we transplanted MC3T3-E1 cell masses onto the CAM. The data showed that Dex exposure reduced the vessel density within the developed cell mass, concomitantly with the downregulation of IGF-1 pathway. We verified that the inhibition of blood vessel formation caused by Dex could be rescued by IGF-1 treatment using the CAM angiogenesis model. Eventually, we demonstrated that the shortened length of the phalanges in the presence of Dex could be reversed by IGF-1 addition. In summary, these findings suggested that the inhibition of Igf-1 signal caused by Dex exposure exerts a detrimental impact on the formation of osteoblasts and angiogenesis, which consequently shortens long bones during osteogenesis. 相似文献
1000.