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Kiranmai Chadipiralla Ji Min Yochim Bindu Bahuleyan Chun-Yuh Charles Huang Franklin Garcia-Godoy Peter E. Murray Eric J. Stelnicki 《Cell and tissue research》2010,340(2):323-333
Multipotent stem cells derived from periodontal ligaments (PDLSC) and pulp of human exfoliated deciduous teeth (SHED) represent
promising cell sources for bone regeneration. Recent studies have demonstrated that retinoic acid (RA) and dexamethasone (Dex)
induce osteogenesis of postnatal stem cells. The objective of this study was to examine the effects of RA and Dex on the proliferation
and osteogenic differentiation of SHED and PDLSC and to compare the osteogenic characteristics of SHED and PDLSC under RA
treatment. SHED and PDLSC were treated with serum-free medium either alone or supplemented with RA or Dex for 21 days. The
proliferation of SHED and PDLSC was significantly inhibited by both RA and Dex. RA significantly upregulated gene expression
and the activity of alkaline phosphatase in SHED and PDLSC. Positive Alizarin red and von Kossa staining of calcium deposition
was seen on the RA-treated SHED and PDLSC after 21 days of culture. The influences of RA on the osteogenic differentiation
of SHED and PDLSC were significantly stronger than with Dex. Supplemention with insulin enhanced RA-induced osteogenic differentiation
of SHED. Thus, RA is an effective inducer of osteogenic differentiation of SHED and PDLSC, whereas RA treatment in combination
with insulin supplementation might be a better option for inducing osteogenic differentiation. Significantly higher cell proliferation
of PDLSC results in greater calcium deposition after 3-week culture, suggesting that PDLSC is a better osteogenic stem cell
source. This study provides valuable information for efficiently producing osteogenically differentiated SHED or PDLSC for
in vivo bone regeneration. 相似文献
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Pattanashetty Ravindra Sathiamma Sulekha Talakkad SathyaPrabha Nityananda Pradhan Trichur Raju Kutty Bindu M. 《Sleep and biological rhythms》2010,8(1):34-41
Sleep and Biological Rhythms - Intense meditation practices influence brain functions in different ways and at different levels. Earlier studies have shown that meditation practices help to... 相似文献
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Folate-targeted immunotherapy effectively treats established adjuvant and collagen-induced arthritis
Paulos CM Varghese B Widmer WR Breur GJ Vlashi E Low PS 《Arthritis research & therapy》2006,8(3):R77-10
Activated macrophages express a cell surface receptor for the vitamin folic acid. Because this receptor is inaccessible or
not measurably expressed on other normal cells, folic acid has been recently exploited to selectively deliver attached radio-emitters
to sites of activated macrophage accumulation, allowing scintigraphic imaging of inflamed joints and organs of arthritic rats.
We demonstrate here that folate-linked haptens can also be targeted to activated macrophages, decorating their cell surfaces
with highly immunogenic molecules. Under conditions in which the rodent has already been immunized against keyhole limpet
hemocyanine-(fluorescein isothiocyanate) FITC, activated macrophages are eliminated. Administration of folate-FITC conjugates
to rodents with experimental arthritis attenuates (a) systemic and peri-articular inflammation, (b) bone and cartilage degradation,
and (c) arthritis-related body weight loss. Treatment with folate-hapten conjugates is comparable to methotrexate, etanercept,
anakinra, and celecoxib at alleviating the symptoms of arthritis. We conclude that reduction of activated macrophages by folate-targeted
immunotherapy can ameliorate the symptoms of arthritis in two rodent models of the disease. 相似文献
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Thiruganesh Ramasamy Shankar Munusamy Hima Bindu Ruttala Jong Oh Kim 《Biotechnology journal》2021,16(2):1900408
Nucleic acid-based therapies are promising therapeutics for the treatment of several systemic disorders, and they offer an exciting opportunity to address emerging biological challenges. The scope of nucleic acid-based therapeutics in the treatment of multiple disease states including cancers has been widened by recent progress in Ribonucleic acids (RNA) biology. However, cascades of systemic and intracellular barriers, including rapid degradation, renal clearance, and poor cellular uptake, hinder the clinical effectiveness of nucleic acid-based therapies. These barriers can be circumvented by utilizing advanced smart nanocarriers that efficiently deliver and release the encapsulated nucleic acids into the target tissues. This review describes the current status of clinical trials on nucleic acid-based therapeutics and highlights representative examples that provide an overview on the current and emerging trends in nucleic acid-based therapies. A better understanding of the design of advanced nanocarriers is essential to promote the translation of therapeutic nucleic acids into a clinical reality. 相似文献
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Prabakaran Ravichandran Adaikkappan Periyakaruppan Bindu Sadanandan Vani Ramesh Joseph C. Hall Olufisayo Jejelowo Govindarajan T. Ramesh 《Journal of biochemical and molecular toxicology》2009,23(5):333-344
Carbon nanotubes (CNTs), the most promising material with unique characteristics, find its application in different fields ranging from composite materials to medicine and from electronics to energy storage. However, little is known about the mechanism behind the interaction of these particles with cells and their toxicity. So, here we investigated the adverse effects of multiwalled CNTs (MWCNTs) in rat lung epithelial (LE) cells. The results showed that the incubation of LE cells with 0.5–10 μg/mL of MWCNTs caused a dose‐ and time‐dependent increase in the formation of free radicals, the accumulation of peroxidative products, the loss of cell viability, and antioxidant depletion. The significant amount of incorporation of dUTPs in the nucleus after 24 h confirms the induction of apoptosis. It was also observed that there is an increase in the activity of both caspases‐3 and caspase‐8 in cells, with increases in time and the concentration of MWCNTs. No significant incorporation of dUTPs was observed in cells, incubated with z‐VAD‐fmk , which confirmed the role of caspases in DNA fragmentation. The present study reveals that MWCNTs induced oxidative stress and stimulated apoptosis signaling pathway through caspase activation in rat LE cell lines. © 2009 Wiley Periodicals, Inc. J Biochem Mol Toxicol 23:333–344, 2009; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20296 相似文献
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Treatment of coenzyme Q with ozone yielded a degradation product having unmodified ring that retained its spectral characteristics and a truncated side-chain that made it water-soluble. This derivative, but not the intact lipid-quinone, showed relaxation of phenylephrine-contracted rat arterial rings. This effect offers an explanation for the known hypotensive action of exogenous coenzyme Q regardless of its side-chain length. 相似文献
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