东北地区生态环境中的Se及其生态效应

对东北地区不同环境要素Ｓｅ的分布与转化规律的研究表明,基岩、土壤、粮食和动物毛Ｓｅ含量分别为０．１２、０．１５０－０．５４０、０．００９６－０．０７６５和０．０４０５－０．１４１４μｇ．ｇ＾－１;城市和农村儿童发Ｓｅ含量为０．４６０和０．１８２μｇ．ｇ＾－１。根据不同要素Ｓｅ含量和生态效应,可将该区分为Ｓｅ适宜区、缺乏区和过渡区３个１级区,每个１级区又分为高Ｓｅ源区的低Ｓｅ源区。     

赣榆县滨海通榆运河综合效益型防护林的优化模式

在评价赣榆县滨海通榆运河河岸堤顶,堤腰,堤底三个立地条件分异性的基础上,进行了１１个树种（品种）的引种试验和间作物配置,建立了１１种立体种植模式,分析了各个立地条件下的林木生长表现和经济效益,从生态、经济和社会的综合效益考虑,设计了河岸利用的最优模式;堤顶为香椿＋小麦＋大豆;堤腰为Ｉ－６９杨＋小麦＋大豆;堤底为Ｉ－１０５杨＋杞柳。预期经济效益可比原有纯杨树型模式提高４０．９％。     

植物定植图微机显示与管理系统

介绍了运用软件工程的方法和数据库技术研究开发的南京中山植物园植物定植图微机显示与管理系统。详细介绍了系统的目标,系统的实现,应用软件的研制及应用效果。     

狂犬病毒“北京株”在地鼠肾传代细胞中增殖的电镜观察

用透射电子显微镜观察术,对狂犬病固定毒“北京株”在地鼠肾传代细胞（ＢＨＫ２１）中增殖的形态学变化进行了研究。在受感染的细胞中有大量子弹状和长杆状的病毒颗粒。大多数病毒在扩张的内质网膜上以出芽的方式发生,凸向内质网腔内并逐渐发育成子弹状和杆关的成熟病毒。含病的内质,多周围常伴有颗粒或丝状均质区域,少数病毒在细胞膜上芽生。细胞间隙中亦可见病毒。还见病毒在细胞核膜内、外层上芽生,核周围间隙中许多病毒,有     

真菌细胞溶壁酶的产酶培养和应用效果

从土样中分离的７１株木霉（Ｔｒｉｃｈｏｄｅｒｍａ ｓｐ．）筛选出一株分解几丁质和葡聚糖能力较强的菌株。该菌株在麸皮、稻草粉、硫酸铵和诱导物为主成份的固体培养基上,经过２５℃、８４ｈ的培养,产生较高的真菌细胞溶壁酶,能溶解酵母、毛霉、黑曲霉及食用菌等丝状菌丝体的细胞壁,形成原生质体,经选用酵母、毛霉等进行原生质体再生,效果较优。     

无色素腺棉籽蛋白粉与棉子油的制备

低棉酚棉籽为高蛋白含油的植物资源,适宜于水溶法制备油及蛋白粉,出油率一般可达９５％。     

Evidence that 5-HT2 antagonism elicits a 5-HT3-mediated increase in dopamine transmission

Amperozide, a novel atypical antipsychotic drug with few extrapyramidal side effects, is a strong serotonin₂ (5-HT₂) antagonist but has low affinity for dopamine receptors in vitro. The effect of amperozide on the dopaminergic synapse was studied with an in vivo microdialysis technique using anesthetized male Sprague-Dawley rats. Following implantation of dialysis probes into the striatum and nucleus accumbens (NuAc), amperozide was intravenously infused as six consecutive incremental doses (0.5, 0.5, 1.0, 2.0, 4.0 and 8.0 mg/kg) at intervals of 15 min. From the beginning of drug infusion, perfusates were collected in fractions every 30 min throughout a total period of 120 min. The samples were then immediately analyzed by high-performance liquid chromatography with electrochemical detection. Amperozide induced a dose-related elevation of dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindolacetic acid (5-HIAA) levels in both areas.p-Chlorophenylalanine (pCPA) pretreatment abolished the production of 5-HIAA in both areas and attenuated the amperozide-induced rise of DOPAC but not of dopamine. After pretreatment with an intravenous 5-HT₃ antagonist, MDL 72222, the amperozide-induced changes in dopamine, DOPAC and 5-HIAA in both areas were lower than in the saline control group. Preliminary data showed that afterpCPA pretreatment, incremental concentrations of the 5-HT₃ agonist 1-(m-chlorophenyl)-biguanide perfused via the probe also produced significant elevation of dopamine and DOPAC levels in these two areas. Taken together, these results suggest that amperozide may directly block 5-HT₂ receptors in the striatum and NuAc, thereby enhancing 5-HT transmission. The enhanced 5-HT transmission may activate postsynpatic 5-HT₃ receptors located on the dopaminergic terminals, leading to changes in dopamine transmission in these two areas.     

Expression of the BnmNAP subfamily of napin genes coincides with the induction of Brassica microspore embryogenesis

Brassica napus cv. Topas microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress treatment. We show that this switch in developmental pathways is accompanied by the induction of high levels of napin seed storage protein gene expression. Changes in the plant growth or microspore culture conditions were not by themselves sufficient to induce napin gene expression. Specific members of the napin multigene family were cloned from a cDNA library prepared from microspores that had been induced to undergo embryogenesis. The majority of napin clones represented three members (BnmNAP2, BnmNAP3 and BnmNAP4) that, along with a previously isolated napin genomic clone (BngNAP1), constitute the highly conserved BnmNAP subfamily of napin genes. Both RNA gel blot analysis, using a subfamily-specific probe, and histochemical analysis of transgenic plants expressing a BngNAP1 promoter--glucuronidase gene fusion demonstrated that the BnmNAP subfamily is expressed in embryogenic microspores as well as during subsequent stages of microsporic embryo development.     

Purification and characterization of recombinant human pro-urokinase produced in silkworm using a baculovirus vector

Summary A recombinant baculovirus (BmNPV-pk2) was constructed by inserting the human pro-urokinase(pro-UK) cDNA into the genome of baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) adjacent to the strong polyhedrin promoter. The recombinant virus replicated in silkworm larvae, which synthesized 30g pro-UK/ml in the haemolymph within 4 days post-infection. Purification to near homogeneity was accomplished by fractional precipitation with ammonium sulphate and immunoaffinity chromatography with an overall yield of 23% and a specific activity of 100,000IU/mg in fibrin plate assay. This purified product was comprised of a single chain protein with approximately Mr. 50kDa as determined by SDS-PAGE gel. The N-Terminal amino acids sequence revealed that the secretion signal of pro-UK was correctly processed.     

植物细胞离析酶的制备和应用

用 Aspergillus sp．A-19菌经固体发酵研制成一种新的植物细胞离析酶(SeparatasezA—P)。其离析单细胞的酶活力平均为70 767u／g,有效作用的pH在3．0—7．0,温度为20—45℃。发酵培养基配方是麸皮：桔皮粉：(NH₄)₂SO₄(w／w)为100:100:O．63,料水比为1 :2．0,培养适宜条件为25℃、60小时。