全文获取类型
收费全文 | 1023篇 |
免费 | 79篇 |
专业分类
1102篇 |
出版年
2023年 | 4篇 |
2022年 | 13篇 |
2021年 | 16篇 |
2020年 | 10篇 |
2019年 | 13篇 |
2018年 | 16篇 |
2017年 | 4篇 |
2016年 | 18篇 |
2015年 | 40篇 |
2014年 | 31篇 |
2013年 | 68篇 |
2012年 | 48篇 |
2011年 | 64篇 |
2010年 | 31篇 |
2009年 | 37篇 |
2008年 | 49篇 |
2007年 | 44篇 |
2006年 | 40篇 |
2005年 | 43篇 |
2004年 | 41篇 |
2003年 | 52篇 |
2002年 | 30篇 |
2001年 | 31篇 |
2000年 | 34篇 |
1999年 | 29篇 |
1998年 | 13篇 |
1997年 | 6篇 |
1996年 | 9篇 |
1995年 | 7篇 |
1994年 | 10篇 |
1992年 | 31篇 |
1991年 | 12篇 |
1990年 | 25篇 |
1989年 | 17篇 |
1988年 | 15篇 |
1987年 | 18篇 |
1986年 | 15篇 |
1985年 | 12篇 |
1984年 | 15篇 |
1983年 | 6篇 |
1982年 | 7篇 |
1981年 | 5篇 |
1980年 | 11篇 |
1979年 | 7篇 |
1978年 | 9篇 |
1977年 | 5篇 |
1976年 | 9篇 |
1974年 | 4篇 |
1973年 | 7篇 |
1972年 | 3篇 |
排序方式: 共有1102条查询结果,搜索用时 7 毫秒
21.
22.
A Pal T Ramamurthy R K Bhadra T Takeda T Shimada Y Takeda G B Nair S C Pal S Chakrabarti 《Applied and environmental microbiology》1992,58(8):2485-2489
A collection of 521 environmental isolates of Vibrio cholerae which were previously examined by the suckling mouse assay and found to be negative for the heat-stable enterotoxin NAG-ST were reassessed by a recently developed DNA probe for NAG-ST. A total of 12 (2.3%) of the isolates hybridized with the NAG-ST probe. By using a cholera toxin (CT) DNA probe, the CT gene was detected in six of the strains in the collection, although none of the isolates of V. cholerae non-O1 hybridized with both of the toxin probes. All of the NAG-ST and CT probe-positive strains were hemolysin positive. Thirty-fold-concentrated supernatants of the three representative NAG-ST DNA probe-positive V. cholerae non-O1 strains gave positive fluid accumulation ratios in the suckling mouse assay even after heating (100 degrees C for 5 min) and also inhibited the binding of a NAG-ST monoclonal antibody to the bound NAG-ST in a competitive enzyme-linked immunosorbent assay (ELISA). Likewise, all six CT probe-positive V. cholerae non-O1 strains produced in vitro CT when examined by the CT bead ELISA. HindIII digest patterns of chromosomal DNA from the representative NAG-ST gene-positive strains were visually indistinguishable. Between the groups of NAG-ST probe-positive strains examined, there was a variation in the hybridizable fragments, with one group of strains exhibiting a hybridizable fragment similar to that of the NRT 36 reference strain; a smaller HindIII fragment hybridized with the NAG-ST probe in the other group of strains.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
23.
A new antifungal antibiotic named acmycin was isolated from a soil streptomycete. Detailed comparative taxonomic studies showed
that the organism differed from three related species of streptomycetes. The organism was referred to asStreptomyces sp. AC2. The isolated antibiotic appears to be of polyene nature. 相似文献
24.
Phosphoinositol kinase, the key enzyme responsible for the biosynthesis of higher inositol phosphates has been isolated from the cotyledons of mung beans germinated for 24 hr and has been resolved into two different forms, phosphoinositol kinase A and phosphoinositol kinase B. Both forms were purified to homogeneity and characterized. The Km values for ATP with phosphoinositol kinase A (1.78 × 10?4 M) and phosphoinositol kinase B (3.12 × 10 ?5 M) showed that phosphoinositol kinase B had a greater affinity for ATP. ATP could be partially replaced as phosphate donor by UTP and phosphoenolpyruvate in the case of phosphoinositol kinase A but not in the case of phosphoinositol kinase B. 相似文献
25.
The molecular conformation of d1-8-isotestosterone has been determined crystallographically. Crystals of the title compound belong to the space group P21/c with a = 11.449(4), b = 10.962(4), c = 25.860(5) , β = 100.95(4)0, with two molecules in the asymmetric unit. The structure has been refined to a final R value of 0.052 for 2227 reflections. Unlike testosterone, which is a flat molecule, its 8-isomer has a folded conformation. The conformations of the ring-B in the two crystallographically independent molecules (A and B) correspond to the twist form and differ significantly from one another. 相似文献
26.
27.
28.
T W Meade R Chakrabarti A P Haines W R North Y Stirling 《BMJ (Clinical research ed.)》1979,1(6157):153-156
As part of a study to determine the extent to which the haemostatic system is implicated in the onset of clinically manifest ischaemic heart disease, characteristics influencing fibrinolytic activity (FA) and plasma fibrinogen concentrations were examined in 1601 men aged 18-64 and 707 women aged 18-59 in several occupational groups in North-west London. In men FA noticeably decreased till the age of about 58, when there was a small rise. In women a small increase in FA between 18 and about 40 was followed by a slightly larger fall between 40 and 59. There was a pronounced negative association of FA with obesity. FA was significantly less in smokers than non-smokers, though the effect was not large. FA increased with alcohol consumption. FA in men appeared to be greatest in the lower social classes, and men on night shift had poorer FA than those on day work. FA was greater in women using oral contraceptives than in those not using these preparations. In both sexes FA increased with exercise, but there were no associations between any of the characteristics studied and the increase. Plasma fibrinogen concentrations increase with age and obesity, are higher in smokers than non-smokers, and fall with alcohol consumption. In women the concentrations are higher in those using oral contraceptives. The general epidemiology of FA and plasma fibrinogen concentrations suggests that they may well be implicated in the pathogenesis of ischaemic heart disease. 相似文献
29.
Purification and characterization of myo-inositol hexaphosphate-adenosine diphosphate phosphotransferase from Phaseolus aureus 总被引:2,自引:0,他引:2
S Biswas I B Maity S Chakrabarti B B Biswas 《Archives of biochemistry and biophysics》1978,185(2):557-566
myo-Inositol hexaphosphate adenosine diphosphate phosphotransferase transfers phosphate from myo-inositol hexaphosphate to adenosine diphosphate to synthesize adenosine triphosphate. This enzyme has been isolated and purified from ungerminated mungbean seeds and found to be different from guanosine diphosphate phosphotransferase. A purification of about 200-fold with 15% recovery has been obtained. The optimal pH of the reaction is 7.0 and is dependent on the presence of a divalent cation, i.e., Mg2+ and Mn2+. The Km value for myo-inositol hexaphosphate has been found to be 0.41 × 10?4m and V is 90.0 nmol of Pi transferred per milligram of protein per 20 min. Km for ADP is 0.88 × 10-4m and V is 83.3 nmol of phosphorus transferred to ADP per milligram of protein per 20 min. The ADP phosphotransferase reaction is reversible to the extent of about 50% of the forward reaction. dADP is partly effective as an acceptor but other ribonucleoside mono- and diphosphates cannot substitute for ADP. The products ATP and myo-inositol pentaphosphate have been confirmed by several criteria. It has also been shown that this enzyme transfers phosphate only from a specific phosphoryl group (C-2 position) of myo-inositol hexaphosphate for the synthesis of ATP and 1,3,4,5,6-myo-inositol pentaphosphate or pentakis (dihydrogen phosphate). 相似文献
30.