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11.
Relative importance of osmotic-stress and ion-specific effects on ABA-mediated inhibition of leaf expansion growth in Phaseolus vulgaris 总被引:2,自引:1,他引:1
The osmotic and ion-specific components of salt-induced inhibition of leaf expansion growth were investigated in beans grown from 12 h to several days in either NaCl-containing solution cultures, an isosmotic concentrated macronutrient solution, or a vermiculite–compost mixture with low Na+ but high Cl– availability. Inhibition of leaf expansion and leaf ABA increase was more intense in the NaCl than in the isosmotic macronutrient treatment. Root Na+ was highly correlated to inhibition of leaf expansion and leaf or xylem sap ABA. When Na+ was sequestered in soil, salinized plants showed no reduction in leaf expansion or ABA increase, regardless of the presence of high leaf Cl– concentrations. Stomatal conductance exhibited an exponential relationship with the reciprocal value of xylem sap ABA. Our results indicate that an ion-specific effect caused by Na+ in roots may account for an ABA-mediated reponse of both stomatal closure and leaf expansion inhibition. 相似文献
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B.P. Girish CH. Swetha P. Sreenivasula Reddy 《Biochemical and biophysical research communications》2014
The objective of the present study was to explore the site of synthesis of vitellogenin (Vtg) in fresh water edible crab, Oziothelphusa senex senex. Vtg cDNA fragments were isolated from the hepatopancreas of female crabs using RT-PCR method, and the deduced amino acid sequence of O. senex senex showed more than 60% identity with other brachyuran Vtg sequences. RT-PCR analysis showed that Vtg mRNA can be detected only in hepatopancreas of female Oziothelphusa but not in other tissues including eyestalks, Y-organs, mandibular organs, thoracic ganglion, hypodermis and ovary. Antibodies were raised against vitellin purified from the ovary of O. senex senex. Immunoprecipitation analysis revealed the presence of Vtg in the hepatopancreas of vitellogenic stage I females and in the hemolymph, hepatopancreas and ovary extracts from vitellogenic stage II females but absent in hemolymph and hepatopancreas extract of males. These results suggest that Vtg is synthesized only in hepatopancreas but not in the ovaries of O. senex senex. In addition, Vtg synthesized in hepatopancreas is transported to ovary through hemolymph. 相似文献
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Robbins EM Krupp AJ Perez de Arce K Ghosh AK Fogel AI Boucard A Südhof TC Stein V Biederer T 《Neuron》2010,68(5):894-906
Synaptogenesis is required for wiring neuronal circuits in the developing brain and continues to remodel adult networks. However, the molecules organizing synapse development and maintenance in?vivo remain incompletely understood. We now demonstrate that the immunoglobulin adhesion molecule SynCAM 1 dynamically alters synapse number and plasticity. Overexpression of SynCAM 1 in transgenic mice promotes excitatory synapse number, while loss of SynCAM 1 results in fewer excitatory synapses. By turning off SynCAM 1 overexpression in transgenic brains, we show that it maintains the newly induced synapses. SynCAM 1 also functions at mature synapses to alter their plasticity by regulating long-term depression. Consistent with these effects on neuronal connectivity, SynCAM 1 expression affects spatial learning, with knock-out mice learning better. The reciprocal effects of increased SynCAM 1 expression and loss reveal that this adhesion molecule contributes to the regulation of synapse number and plasticity, and impacts how neuronal networks undergo activity-dependent changes. 相似文献
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CH Balachiranjeevi Naik S. Bhaskar V. Abhilash S. Akanksha B. C. Viraktamath M. S. Madhav A. S. Hariprasad G. S. Laha M. S. Prasad S. M. Balachandran C. N. Neeraja M. Satendra Kumar P. Senguttuvel K. B. Kemparaju V. P. Bhadana T. Ram G. Harika H. K. Mahadeva Swamy S. K. Hajira A. Yugander K. Pranathi M. Anila G. Rekha M. B. V. N. Kousik T. Dilip Kumar R. K. Swapnil Archana Giri R. M. Sundaram 《Molecular breeding : new strategies in plant improvement》2015,35(7):1-12
17.
Bioinformatic characterization of the SynCAM family of immunoglobulin-like domain-containing adhesion molecules 总被引:5,自引:0,他引:5
Biederer T 《Genomics》2006,87(1):139-150
SynCAM 1 (synaptic cell adhesion molecule 1, alternatively named Tslc1 and nectin-like protein 3) belongs to the immunoglobulin superfamily and is an adhesion molecule that operates in a variety of important contexts. Exemplary are its roles in adhesion at synapses in the central nervous system and as tumor suppressor. Here, I describe a family of genes homologous to SynCAM 1 comprising four genes found solely in vertebrates. All SynCAM genes encode proteins with three immunoglobulin-like domains of the V-set, C1-set, and I-set subclasses. Comparison of genomic with cDNA sequences provides their exon-intron structure. Alternative splicing generates isoforms of SynCAM proteins, and diverse SynCAM 1 and 2 isoforms are created in an extracellular region rich in predicted O-glycosylation sites. Protein interaction motifs in the cytosolic sequence are highly conserved among all four SynCAM proteins, indicating their critical functional role. These findings aim to facilitate the understanding of SynCAM genes and provide the framework to examine the physiological functions of this family of vertebrate-specific adhesion molecules. 相似文献
18.
Background
Two-dimensional data colourings are an effective medium by which to represent three-dimensional data in two dimensions. Such "color-grid" representations have found increasing use in the biological sciences (e.g. microarray 'heat maps' and bioactivity data) as they are particularly suited to complex data sets and offer an alternative to the graphical representations included in traditional statistical software packages. The effectiveness of color-grids lies in their graphical design, which introduces a standard for customizable data representation. Currently, software applications capable of generating limited color-grid representations can be found only in advanced statistical packages or custom programs (e.g. micro-array analysis tools), often associated with steep learning curves and requiring expert knowledge. 相似文献19.
Multiple signaling pathways initiate and specify the formation of synapses in the central nervous system. General principles that organize nascent synapses have emerged from the studies in multiple model organisms. These include the synapse-organizing roles of dedicated synaptic adhesion molecules, synaptic signaling following receptor-ligand interactions, and the regulation of synapse formation by secreted molecules. Intracellularly, a range of effectors subsequently regulates signaling steps and cytoskeletal changes. Together, a blueprint of synapse formation is emerging into which these distinct signaling steps will need to be integrated temporally and spatially. 相似文献
20.
Molecular cytogenetic analysis and resequencing of contactin associated protein-like 2 in autism spectrum disorders 总被引:1,自引:0,他引:1
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Bakkaloglu B O'Roak BJ Louvi A Gupta AR Abelson JF Morgan TM Chawarska K Klin A Ercan-Sencicek AG Stillman AA Tanriover G Abrahams BS Duvall JA Robbins EM Geschwind DH Biederer T Gunel M Lifton RP State MW 《American journal of human genetics》2008,82(1):165-173
Autism spectrum disorders (ASD) are a group of related neurodevelopmental syndromes with complex genetic etiology. We identified a de novo chromosome 7q inversion disrupting Autism susceptibility candidate 2 (AUTS2) and Contactin Associated Protein-Like 2 (CNTNAP2) in a child with cognitive and social delay. We focused our initial analysis on CNTNAP2 based on our demonstration of disruption of Contactin 4 (CNTN4) in a patient with ASD; the recent finding of rare homozygous mutations in CNTNAP2 leading to intractable seizures and autism; and in situ and biochemical analyses reported herein that confirm expression in relevant brain regions and demonstrate the presence of CNTNAP2 in the synaptic plasma membrane fraction of rat forebrain lysates. We comprehensively resequenced CNTNAP2 in 635 patients and 942 controls. Among patients, we identified a total of 27 nonsynonymous changes; 13 were rare and unique to patients and 8 of these were predicted to be deleterious by bioinformatic approaches and/or altered residues conserved across all species. One variant at a highly conserved position, I869T, was inherited by four affected children in three unrelated families, but was not found in 4010 control chromosomes (p = 0.014). Overall, this resequencing data demonstrated a modest nonsignificant increase in the burden of rare variants in cases versus controls. Nonetheless, when viewed in light of two independent studies published in this issue of AJHG showing a relationship between ASD and common CNTNAP2 alleles, the cytogenetic and mutation screening data suggest that rare variants may also contribute to the pathophysiology of ASD, but place limits on the magnitude of this contribution. 相似文献