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81.
82.
Computerized microdissection of cellular images 总被引:1,自引:0,他引:1
83.
M Bibbo D H Kim H Galera-Davidson C di Loreto H E Dytch 《Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology》1990,12(2):85-90
Novel software was developed to perform quantitative measurements of architectural and nuclear features in tissue sections. A pilot study was then undertaken to determine the diagnostic relevance of these quantitative features in prostatic tissue and the relationship of these objective features to the subjective clues used by practicing pathologists in the grading of prostatic adenocarcinoma. From a group of 82 cases of adenocarcinoma of the prostate with long-term follow-up, a subset of 15 cases that included 5 each in Mostofi grades I, II and III was carefully selected for analysis. Consecutive sections from each case were stained with hematoxylin and eosin or the Feulgen stain for visual and cytometric evaluations, respectively. The most important differences in the objective architectural features observed between the Mostofi grade I and II cases were the number of nuclei per gland and their distance from the glandular center. Significant differences were also noted in gland size and the variation in gland size. The Mostofi grades were also significantly different in terms of quantitative high-resolution features measuring nuclear size and its variation, total nuclear DNA content and the proportion of very aneuploid nuclei. There was a fairly good agreement between many of the subjective diagnostic clues and their corresponding quantitative architectural and nuclear features. This work (1) significantly extended the capabilities of our PC-based microphotometer system to analyze glandular tissue specimens, (2) provided insight into the objective bases for the expert diagnosis of adenocarcinomas of the prostate and (3) gave preliminary evidence of the ability of quantitative architectural features and high-resolution cytometric features to discriminate between the major diagnostic categories of these lesions. 相似文献
84.
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87.
Computer grading of cervical intraepithelial neoplastic lesions. I. Cytologic indices 总被引:1,自引:0,他引:1
In an attempt to derive a computer-assisted grading for cervical intraepithelial neoplastic (CIN) lesions, apparent discrepancies with the visual diagnostic evaluation could be explained from the data structure of the objective assessment. The 40 cases of dysplasia in this study appeared to fall into two subgroups having different curves of progression. A smaller subgroup, visually graded as milder, less-severe CIN, exhibited dysplastic cells with unexpectedly high abnormality indices. A larger subgroup showed dysplastic cells with abnormality indices in agreement with the tissue assessment. 相似文献
88.
89.
Marluce Bibbo Peter H. Bartels Harvey E. Dytch James H. Puls Frederick T. Pishotta George L. Wied 《Cell biochemistry and biophysics》1983,5(1):61-69
Comparison was made between cytophotometric measurements obtained using two data acquisition systems, one a microphotometer
and the other a rapid video camera system, to ascertain whether the degradation of data with the faster video acquisition
system still results in recorded images of sufficient quality to permit computer discrimination between cells of very similar
appearance. Normal-appearing intermediate cells from cases with normal cytology and those from patients with dysplasia or
malignant disease, as well as the subvisual markers within these cells that have rendered them capable of cytophotometric
discrimination, were used for the study. Comparison of the data recorded by the two systems indicates that the diagnostic
information is preserved in the change-over to a full-field, video-rate scanning system, with differences in the data caused
primarily by differences in the spectral response of the two systems. This was reflected in the substantial differences observed
in the color-related features and the lesser differences seen in the textural features, while the morphometric features (outline
and shape) were virtually unaffected. The differences were primarily expressed on a cell-to-cell basis; in sets of about 300
cells, which would be used in patient-to-patient comparisons, the feature values showed remarkable consistency between the
two systems. 相似文献
90.
Some of the clinical capabilities of an inexpensive, microcomputer-based DNA-cytometry system are described. A variety of applications is illustrated: ploidy assessment, which provides significant information for the management of patients with tumors from almost any organ site; quality control of cytologic and histologic diagnoses; determination of tumor heterogeneity; monitoring of cell profile changes during therapy; and quantitation of immunocytochemical staining. 相似文献