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91.
Jonathan P. Belman Rachel R. Bian Estifanos N. Habtemichael Don T. Li Michael J. Jurczak Abel Alcázar-Román Leah J. McNally Gerald I. Shulman Jonathan S. Bogan 《The Journal of biological chemistry》2015,290(7):4447-4463
Insulin causes the exocytic translocation of GLUT4 glucose transporters to stimulate glucose uptake in fat and muscle. Previous results support a model in which TUG traps GLUT4 in intracellular, insulin-responsive vesicles termed GLUT4 storage vesicles (GSVs). Insulin triggers TUG cleavage to release the GSVs; GLUT4 then recycles through endosomes during ongoing insulin exposure. The TUG C terminus binds a GSV anchoring site comprising Golgin-160 and possibly other proteins. Here, we report that the TUG C terminus is acetylated. The TUG C-terminal peptide bound the Golgin-160-associated protein, ACBD3 (acyl-CoA-binding domain-containing 3), and acetylation reduced binding of TUG to ACBD3 but not to Golgin-160. Mutation of the acetylated residues impaired insulin-responsive GLUT4 trafficking in 3T3-L1 adipocytes. ACBD3 overexpression enhanced the translocation of GSV cargos, GLUT4 and insulin-regulated aminopeptidase (IRAP), and ACBD3 was required for intracellular retention of these cargos in unstimulated cells. Sirtuin 2 (SIRT2), a NAD+-dependent deacetylase, bound TUG and deacetylated the TUG peptide. SIRT2 overexpression reduced TUG acetylation and redistributed GLUT4 and IRAP to the plasma membrane in 3T3-L1 adipocytes. Mutation of the acetylated residues in TUG abrogated these effects. In mice, SIRT2 deletion increased TUG acetylation and proteolytic processing. During glucose tolerance tests, glucose disposal was enhanced in SIRT2 knock-out mice, compared with wild type controls, without any effect on insulin concentrations. Together, these data support a model in which TUG acetylation modulates its interaction with Golgi matrix proteins and is regulated by SIRT2. Moreover, acetylation of TUG enhances its function to trap GSVs within unstimulated cells and enhances insulin-stimulated glucose uptake. 相似文献
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玉米是重要的粮食作物,水稻黑条矮缩病毒(RBSDV)是玉米粗缩病的病原,由其引起的玉米粗缩病给玉米生产造成重大损失。利用人工mi RNA构建抗病毒植物的技术已经在多种植物中被证明有效,但是在玉米中的尝试未见报道。实验根据玉米zea-mi R159a的前体序列和RBSDV基因组中编码功能蛋白的基因和基因沉默抑制子的序列信息设计引物,构建了用于沉默RBSDV编码基因和基因沉默抑制子的ami RNA(Artificial mi RNA)基因。构建p CAMBIA3301-121-ami RNA植物表达载体,利用农杆菌介导法转化玉米自交系综31(Z31)。对转基因玉米进行分子检测,选择mi RNA表达量高的纯合体株系进行自然发病实验,按0-4的分级标准调查玉米粗缩病的严重度。结果表明,转抗粗缩病毒人工mi RNA载体玉米纯合体株系的抗病表现好于野生型玉米,其中针对基因组6的S6-mi R159转基因玉米抗病情况较好。研究表明利用人工mi RNA技术构建抗病毒病玉米新品种是可行的。 相似文献
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Xiao Yang Dai Yaohong Lu Yingying Liu Wei Wang Zhuoren Bian Yinbing 《World journal of microbiology & biotechnology》2011,27(7):1731-1734
Rapid and correct authentication of commercial strains is still important in today’s mushroom industry. Here for the first
time we reported the using of sequence characterized amplified region (SCAR) marker developed from inter-retrotransposon amplified
polymorphism (IRAP) marker to identify Lentinula edodes strain. Genomic polymorphism among 44 shiitake cultivars in China were surveyed by 24 IRAP primer combinations, from which
primer combination LTR1L/MarY1R could generate a unique and reproducible 1712 bp fragment to distinguish strain No. 4 from
other 43 strains. Based on this strain-specific fragment, a SCAR primer pair was designed and its validity was verified by
correctly amplifying a single strain-specific fragment from DNA samples of 100 L. edodes strains. Our study lays the foundation for developing strain-specific SCAR marker by retrotransposon-based marker technique
in fungi. 相似文献
97.
Bian Lei Molan Abdul-Lateef Maddox Ian Shu Quan 《World journal of microbiology & biotechnology》2011,27(4):991-998
Lactobacillus reuteri DPC16 is a human-isolated strain recently patented in New Zealand. The antimicrobial activity of cell-free supernatants from
different fermentation processes, with or without glycerol supplementation was studied. When grown in just MRS broth, the
cultural supernatant significantly inhibited the growth of selected food-borne pathogens, possibly due to acidic effect as
this activity was pH-dependent. The cell-free supernatants from secondary fermentation of DPC16 resting cells in glycerol-supplemented
media have shown very different antimicrobial activities. A very potent antimicrobial activity gradually developed during
the fermentation process which was observed only when growing in MRS-glycerol broth (such supernatant is denoted MRSg). This
strong antimicrobial activity was pH-independent, dose-dependant and affected both Gram-negative and Gram-positive pathogens.
Reuterin detected in MRSg is believed to be responsible for these activities. The susceptibility of the selected pathogens
(grown to stationary phase) to MRSg was tested and found that exposure to MRSg for 180 min led to a significant reduction
in cell viability in all pathogens. These results suggest that this is a reuterin-producing strain, which has potent antimicrobial
activity against both Gram-negative and Gram-positive pathogens. These findings have indicated a clear potential of this novel
strain in industrial applications. 相似文献
98.
Li Li Xiu-Zhi Fan Wei Liu Yang Xiao Yin-Bing Bian 《World journal of microbiology & biotechnology》2011,27(9):2081-2093
Phenotypic traits (physiological characteristics and somatic incompatibility) and genotypic traits (target region amplification
polymorphism TRAP) were used to study the diversity of 32 main cultivars of Auricularia auricula-judae in China. Twenty seven important and stable physiological indexes were evaluated; somatic incompatibility test (SIT) reaction
was described from three aspects: type, pigment, and intensity; 16 pairs of TRAP primer combinations produced 535 unambiguous
and reproducible DNA fragments, of these 524 (97.9%) were polymorphic. Dendrograms were constructed by Unweighted Pair-group
Method with Arithmetic Averages (UPGMA) method, and the principal coordinate analysis (PCO) of the three methods (physiological
characteristics, SIT intensity and TRAP) exhibited similar clustered patterns, revealing that all the tested strains could
be divided into three distinct groups, each of which was correlated with different geographical regions. Most strains originated
from the same area were with a narrow genetic basis and could possibly be domesticated from the local wild-type strains, some
strains were suspected to be synonymous. The grouping information obtained in the present work provides significant information
for further genetic improvement of A. auricula-judae. 相似文献
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100.
Chunxia Zhang Mingdi Bian Hui Yu Qing Liu Zhenming Yang 《Plant Physiology and Biochemistry》2011,49(11):1306-1312
Calcineurin B-like proteins play important roles in the calcium perception and signal transduction of abiotic stress. In this study, the bioinformatic analysis of molecular characteristics of Sorghum bicolor calcineurin B-like protein (SbCBL) revealed that sequences of SbCBL are highly conserved, and most SbCBLs have three typical EF-hands structures. Among the SbCBL proteins, four of which, SbCBL01, 04, 05, 08, have a conserved N-myristoylation domain. Stress-responsive and phytohormone-responsive cis-elements were found in the promoter regions of SbCBL genes. Real-time quantitative polymerase chain reaction (RTqPCR) analysis showed that SbCBL genes have different tissue-specific expression patterns under normal growth conditions in sweet sorghum (Sorghum bicolor L. Moench). Interestingly, when treated with sodium carbonate, SbCBL genes also show various sodium carbonate stress responsive patterns in sweet sorghum seedlings. These results suggest that SbCBLs may participate in regulating sodium carbonate stress-specific cellular adaptation responses and influencing growth and developmental patterns in sweet sorghum. 相似文献